Application of salvianolic acid A in preparation of medicine for preventing thrombotic cerebral ischemia
A technology of cerebral ischemia and salvianolic acid, which is applied in the field of medicine, can solve the problems of no salvianolic acid A, etc., and achieve the effect of protecting cerebral vascular endothelial function, improving nerve function, hemorrhagic transformation, or alleviating the degree of bleeding
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Embodiment 1
[0049] Example 1. Effect of prophylactic administration of salvianolic acid A (10 mg / kg) on the neuroprotective function of rats with acute stroke.
[0050] Experimental materials: male SD rats, weighing 220-260 g, purchased from Weitong Lihua (Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.). Salvianolic acid A powder was provided by the National Center for Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences. Aspirin was purchased from Sigma, USA. Sodium carboxymethyl cellulose was purchased from Sinopharm Chemical Reagent Co., Ltd.
[0051] Experimental grouping: 40 SD rats in total, 10 in each group, were given 0.5% sodium carboxymethylcellulose and aspirin 100mg·kg by intragastric administration to the blank group respectively. -1 And salvianolic acid A 10mg·kg -1 , once in the morning and once in the evening, for 5 consecutive days, and on the 6th day, the cerebral ischemia model was prepared by electrocoagulation autothrom...
Embodiment 2
[0054] Example 2. Effect of prophylactic administration of salvianolic acid A (10 mg / kg) on cerebral hemorrhage in rats with acute stroke.
[0055] Animal modeling and administration methods are the same as in Example 1. After 24 hours of cerebral ischemia, the brain was perfused, coronally sliced, photographed, and the homogenate of the perfused brain tissue was taken, and the absorbance was detected at 400 nm according to the instructions of the hemoglobin detection kit (DIHB-250, Bosch Biotechnology Co., Ltd., USA). Calculate the hemoglobin content in the brain tissue. Hematoxylin-eosin stained tissues were stored in 4% paraformaldehyde solution for hematoxylin-eosin staining.
[0056] The result is as figure 2 As shown, hemorrhagic transformation occurred in the brain tissue after ischemia, and the cerebral hemorrhage was severe in the aspirin prophylactic administration group, while the salvianolic acid A prophylactic administration group reduced the occurrence of ce...
Embodiment 3
[0057] Example 3. Effect of prophylactic administration of salvianolic acid A (10 mg / kg) on vascular endothelial function in rats with acute stroke.
[0058]Animal modeling and administration methods are the same as in Example 1. After 24 hours of cerebral ischemia, blood was collected from the abdominal aorta. After standing at room temperature for 2 hours, it was centrifuged at 1,000×g for 10 minutes. ELISA was used to detect von Willebrand factor in serum (Von Willebrand factor kit was purchased from Elabscience Co., Ltd. ) and von Willebrand factor lyase (von Willebrand factor lyase kit was purchased from Novus Company) indicators.
[0059] The protein in the brain tissue of the ischemic area was taken, added Ripa lysate to fully grind and lyse, centrifuged at 12000rpm, 4°C for 15min, and quantified the protein by BCA method. The samples were processed into samples of equal concentration and equal quality. Denatured at 100°C for 10 minutes and placed in a -20°C refrige...
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