Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus velezensis and application thereof

A bacillus and antagonism technology, applied in the field of microbiology, can solve the problems of losing the control ability of botrytis and anthracnose, environmental pollution, toxicity, etc., and achieve the effects of good development and application prospects, easy industrial production, and simple preparation methods

Pending Publication Date: 2021-12-28
JIANGSU UNIV OF SCI & TECH
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prevention and control, imported drugs such as saccharin are often used to spray, and the control effect is good, but in the case of continuous use, the control effect is reduced, and it is easy to make Botrytis cinerea and Botrytis cinerea resistant to pesticides, and even produce multiple resistances. Zhejiang has gradually lost its ability to control Botrytis cinerea and anthracnose
Moreover, when these chemical pesticides are used, they often cause environmental pollution and ecological damage.
At the same time, these chemical pesticides have certain toxicity. If people eat these fruits and vegetables with chemical pesticide residues for a long time, it may seriously damage human health.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus velezensis and application thereof
  • Bacillus velezensis and application thereof
  • Bacillus velezensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) From the strawberry gray mold fruit collected in Jurong Baitu Town, Zhenjiang, first cut the non-infected part of the strawberry fruit suffering from gray mold, disinfect its surface with 0.1% mercuric chloride solution for 3min, and then Grinding under sterile conditions; screening in LB medium by serial dilution;

[0021] (2) After a single colony appears on the plate, distinguish different colonies according to the difference in colony shape and color, and use an inoculation loop to pick up different colonies respectively and culture them by streaking on the PDA solid medium plate;

[0022] (3) Preliminary screening of antagonistic bacteria: take gray mold blocks of basically the same size and place them in the center of the PDA plate, use an inoculation loop to take the bacteria cultured by streaking and draw a shorter line around the mold block, and do a total of Streak the four kinds of bacteria, place them in a constant temperature incubator at 30°C for 5 day...

Embodiment 2

[0028] The Veles bacillus (Bacillus velezensis) ZJK2 that embodiment 1 is isolated is carried out constant temperature culture on LB medium, and colony color is pale yellow, and shape is circular and diameter is smaller, and surface is smooth and moist, has bulge, More transparent, with neat edges, see figure 1 .

[0029] Physiological and biochemical experiments showed that the contact enzyme test, VP test, and citrate utilization test were all positive, and the MR test, H 2 S production tests were all negative, see Table 1 for details.

[0030] Table 1 Physiological and biochemical test results of biocontrol bacteria ZJK2

[0031]

[0032] Note: "+" means positive; "-" means negative

[0033] The DNA of Bacillus velezensis ZJK2 was extracted, and the 16S rRNA gene sequencing was carried out after the fragments were determined by PCR reaction. The sequences were compared on the NCBI website Blast and a phylogenetic tree was constructed ( figure 2 ), combined with morp...

Embodiment 3

[0035] First, pre-treat the leaves on the collected strawberry plants, soak the leaves in water to remove some impurities on the surface of the leaves, and gently wipe the surface of the leaves with 75% alcohol cotton balls to disinfect the surface of the leaves. Then inoculate the strawberry leaves with Botrytis cinerea to infect them with Botrytis cinerea. The specific method is to use a sterile needle to draw 5 1cm-long wounds on both sides of the main vein of the leaves, and then soak the leaves in a pre-prepared 1× 10 6 pcs / mL Botrytis cinerea spore suspension for 5 seconds, then take it out and let it dry naturally for 2 hours. Finally, the strawberry leaves after drying were soaked in 10-fold fermented liquid for 15 seconds and taken out respectively, and three leaves were repeated during the test. Do the same treatment as above with sterile water as a blank control group, and the blank control group also repeats three leaves. Insert all treated strawberry leaves into...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses bacillus velezensis and application thereof. The strain is named as bacillus velezensis ZJK2, is preserved in the China General Microbiological Culture Collection Center on June 8, 2020, and has the preservation number of CGMCC No. 20037. The strain, a thallus prepared by liquid fermentation and a sterile fermentation broth have a good antagonistic effect on gray mold or anthracnose; and the biological agent can achieve a remarkable prevention and treatment effect on gray mold and anthracnose of mulberries, grapes, strawberries and the like, has the advantages of being green, safe, environmentally friendly and the like compared with a traditional chemical agent, and has wide application prospects in the biological prevention and treatment aspect of the gray mold and the anthracnose.

Description

technical field [0001] The invention belongs to the field of microbiology, and in particular relates to a bacillus Velez and its application. Background technique [0002] At present, the control of botrytis and anthracnose is mainly based on chemical means. Botrytis and anthracnose are considered to be easy to produce drug resistance. In the prevention and control, imported drugs such as saccharin are often used to spray, and the control effect is good, but in the case of continuous use, the control effect is reduced, and it is easy to make Botrytis cinerea and Botrytis cinerea resistant to pesticides, and even produce multiple resistances. Due to the lack of medicinal properties, Zhejiang gradually lost its ability to control gray mold and anthracnose. And when these chemical pesticides are used, they often cause environmental pollution and ecological damage. At the same time, these chemical pesticides have certain toxicity. If people eat these fruits and vegetables with...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/22
Inventor 杨统一翟德丽黄锡春胡毅翀刘典三张正杨谌剑许明敏杨庆根邱荣俊徐信超赵玉媛李静
Owner JIANGSU UNIV OF SCI & TECH
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com