Composition for treating Helicobacter pylori and its preparation method and application
A Helicobacter pylori and composition technology, which is applied in the field of Helicobacter pylori treatment composition and its preparation, can solve the problems that the Helicobacter pylori inhibitory effect is not obvious, and the bacteriostatic effect needs to be improved, so as to alleviate the problem of drug resistance, Less drug resistance, excellent effect
Active Publication Date: 2022-06-03
右江民族医学院
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Problems solved by technology
Zuojin Wan is derived from "Danxi Xinfa". It is composed of Coptis chinensis and Evodia rutaecarpa. The dosage ratio is 6:1. Infection, etc. However, according to the drug sensitivity test, the minimum inhibitory concentration (MIC) of Zuojinwan water extract is 50-100μg / mL, and its inhibitory effect on Helicobacter pylori is not obvious, and the antibacterial effect needs to be improved
Method used
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Embodiment 2
[0024] The inhibitory effect of the present invention on Helicobacter pylori is further described in detail by the following examples.
[0026] 1.1 Sample
[0027] The compositions were prepared with Examples 1 and 2, respectively.
[0032] 1.4 main instruments: three-gas incubator, centrifuge, microplate reader, electronic balance and the like.
[0033] 1.5 consumables: EP tube, Tip head, centrifuge tube etc.
[0043]
[0045]
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[0062] (2) Inoculated into 96-well plate: 100 μL per well, and the same sample was repeated 3 times.
[0063] (3) culturing in a 37°C incubator for 24 hours.
[0065] (5) culturing in a 37°C incubator for 24 hours.
[0066] (6) Add 10 μL of CCK8, tap and mix, and culture for 4 hours.
[0080] (5) The result is judged to be the MIC with the lowest drug concentration that completely inhibits bacterial growth in the small hole. When positive control wells
[0083]
[0086] (1) Use Helicobacter pylori G27 strain to detect the drug r...
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A composition for treating Helicobacter pylori and its preparation method and application, preparation of water extract: Coptidis rhizome and Evodia rutaecarpa in a weight ratio of 6:1, add water for soaking and decocting, after each decoction is completed, filter to obtain filtrate, and add water Decoct again, combine the filtrate three times, concentrate, vacuum dry, store in a refrigerator at ‑20°C after drying, and store for later use; dissolve drugs: weigh arctigenin and tanshinone Ⅰ and dissolve them in absolute ethanol, so that the final concentration is 4mg / mL, weigh and dissolve the prepared water extract in sterile water so that the final concentration is 4mg / mL; combination drug: mix the water extract, arctigenin, and tanshinone I according to the mass ratio of 100:8:8 Together, the composition is obtained. The invention successfully prepares a traditional Chinese medicine for treating Helicobacter pylori and a composition of Chinese medicine components. The composition has obvious inhibitory effect on Helicobacter pylori both in vivo and in vitro, has specific action, low toxicity, and is not easy to produce drug resistance.
Description
A composition for treating Helicobacter pylori and its preparation method and application technical field The invention belongs to the field of medicine, be specifically related to a kind of composition for the treatment of Helicobacter pylori and preparation method and application thereof use. Background technique Helicobacter pylori (Helicobacterpylori, Hp) is an important disease such as gastritis, peptic ulcer and gastric cancer. etiology. H. pylori now infects more than half of the world's population. At present, the Hp eradication programs at home and abroad mainly include standard three Antibiotics, non-bismuth quadruple (PPI + 3 kinds of antibacterial drugs), bismuth quadruple (PPI + bismuth + 2 kinds of antibacterial drugs), etc. Antibacterial drugs are mainly Including metronidazole, clarithromycin, levofloxacin, etc. But with the widespread use of antibiotics for treatment, H. pylori resistance is growing The higher the eradication rate, the lower the...
Claims
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IPC IPC(8): A61K36/754A61P31/04A61K31/343A61K31/365
CPCA61K36/718A61K36/754A61K31/365A61K31/343A61P31/04A61K2300/00Y02A50/30
Inventor 黄衍强李如佳周文婷李琛妍黄雯董蕙华黄凯黎何继凯吴童
Owner 右江民族医学院