Preparation method of composite carrier, composite carrier and raceme resolution method of composite carrier
A composite carrier and racemate technology, which is applied in the directions of biochemical equipment and methods, enzymes, etc., which are fixed on or in inorganic carriers, can solve problems such as instability of lipase, and achieve good stability and repeatability. Recovery and reuse, the effect of improving catalytic activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0030] Dissolve 200mg of polyethyleneimine in 8mL of DMF, in a 50mL reaction tube, control the temperature at 40°C, stir for 30min, then add 2mL of glutaraldehyde, react at 40°C for 4h, filter and wash with DMF three times, then use Washed three times with deionized water, freeze-dried to obtain cross-linked PEI;
[0031] Dissolve 1.19g of zinc nitrate hexahydrate and 0.322g of 2-aminoterephthalic acid in 30mL of DMF, add 833μL of triethylamine, stir at room temperature for 2h, filter out the precipitate and wash it three times with DMF, soak in dichloromethane 24h, vacuum drying at 70°C for 12h to give NH 2 - MOF-5 powder;
[0032] 100mg of NH 2 -MOF-5 was dispersed in 5 mL of dichloromethane, 300 μL of 1,3-diisopropylcarbodiimide salt was added, stirred at 5°C for 2 hours, the supernatant was removed by centrifugation, and the precipitate was washed with dichloromethane and acetone, respectively. and ice water three times each to obtain the activated NH 2 -MOF-5; in 16mL...
Embodiment 2
[0035] Obtain cross-linked PEI with the preparation method of Example 1;
[0036] Obtain NH with the preparation method of embodiment 1 2 - MOF-5 powder;
[0037] 500mg of NH 2 -MOF-5 was dispersed in 5 mL of dichloromethane, added 25 μL of 1,3-diisopropylcarbodiimide salt, stirred at 1°C for 20 min, centrifuged to remove the supernatant, and the precipitate was washed with dichloromethane and acetone respectively and ice water three times each to obtain the activated NH 2 -MOF-5; in 16mL DMF, add the above activated NH 2 - MOF-5, add 200 mg of cross-linked PEI, stir at 1 °C for 1 h, filter out the precipitate, wash with DMF, and freeze-dry to obtain a tan solid, which is the composite carrier;
[0038] Take 100 mg of the composite carrier obtained above, 100 mg of Pseudomonas cepacia lipase, stir well in 16 mL of n-hexane at 25°C for 12 h, filter out the precipitate and wash it three times with n-hexane, freeze-dry to obtain the immobilized enzyme.
Embodiment 3
[0040] Obtain cross-linked PEI with the preparation method of Example 1;
[0041] Obtain NH with the preparation method of embodiment 1 2 - MOF-5 powder;
[0042] 5mg of NH 2 -MOF-5 was dispersed in 5 mL of dichloromethane, 0.05 μL of 1,3-diisopropylcarbodiimide salt was added, stirred at 30°C for 5 h, the supernatant was removed by centrifugation, and the precipitate was washed with dichloromethane, Wash with acetone and ice water three times each to obtain activated NH 2 -MOF-5; in 16mL DMF, add the above activated NH 2- MOF-5, and add 16mg of cross-linked PEI, stir at 30°C for 24h, filter out the precipitate, wash with DMF, and freeze-dry to obtain a tan solid which is the composite carrier;
[0043] Take 100 mg of the composite carrier obtained above, 100 mg of Pseudomonas cepacia lipase, stir well in 16 mL of n-hexane at 25°C for 12 h, filter out the precipitate and wash it three times with n-hexane, freeze-dry to obtain the immobilized enzyme.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More