Bactericide composition and preparation method thereof

The technology of a fungicide composition and mixture, which is applied in the field of pesticides, can solve the problems of narrow disease prevention spectrum and poor effect, and achieve the effect of significant control effect, wide disease prevention spectrum and good synergistic effect

Active Publication Date: 2022-01-04
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] On the other hand, the existing fungicides have a narrow spectrum of disease prevention and poor efficacy.

Method used

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  • Bactericide composition and preparation method thereof
  • Bactericide composition and preparation method thereof
  • Bactericide composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Pesticide Composition Bacteriostatic Effect Experiment

[0041] 1 test material

[0042] 1.1 The formula of the pesticide composition is:

[0043] Test group: 0.5 parts of Bacillus Velez HAB-2 extract, 50 parts of ε-polylysine, 30 parts of glycine, 1.5 parts of fucoidan oligosaccharide;

[0044] Contrast group 1: 50 parts of ε-polylysine, 30 parts of glycine, 1.5 parts of fucoidan oligosaccharide;

[0045] Contrast group 2: 0.5 parts of Bacillus Velez HAB-2 extract, 30 parts of glycine, 1.5 parts of fucoidan oligosaccharide;

[0046] Contrast group 3: 0.5 parts of Bacillus Velez HAB-2 extract, 50 parts of ε-polylysine, 1.5 parts of fucoidan oligosaccharide;

[0047] Contrast group 4: 0.5 parts of Bacillus Velez HAB-2 extract, 50 parts of ε-polylysine, 30 parts of glycine;

[0048] Blank control group: water

[0049] Among them, the specific preparation method of the Bacillus velei HAB-2 extract can be found in CN 201510061973.0.

[0050] 1.2 Pathogen: An...

Embodiment 2

[0060] The present embodiment provides a kind of bactericide composition, and its formula is as follows:

[0061] 0.25 parts of Bacillus Velez HAB-2 extract, 40 parts of ε-polylysine, 26 parts of glycine, 5 parts of fucoidan oligosaccharide, 5.25 parts of citric acid, 8 parts of glycerol, 6.5 parts of Tween 20, agricultural Milk 500 9 parts;

[0062] The preparation method is as follows: first mix the extract of Bacillus veleisi HAB-2, ε-polylysine, glycine, and alginate oligosaccharide, then add citric acid, heat (60°C) and stir evenly, then add glycerol in turn , Tween 20, and Nongru 500, and stir evenly to obtain a suspended finished product.

Embodiment 3

[0064] The present embodiment provides a kind of bactericide composition, and its formula is as follows:

[0065] 0.45 parts of Bacillus Velez HAB-2 extract, 45.5 parts of ε-polylysine, 27 parts of glycine, 2.25 parts of fucoidan oligosaccharide, 4.5 parts of citric acid, 6.6 parts of glycerol, 5.7 parts of Tween 20, agricultural Milk 500 8 parts;

[0066] The preparation method is as follows: first mix the extract of Bacillus veleisi HAB-2, ε-polylysine, glycine, and alginate oligosaccharide, then add citric acid, heat (60°C) and stir evenly, then add glycerol in turn , Tween 20, and Nongru 500, and stir evenly to obtain a suspended finished product.

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Abstract

The invention discloses a bactericide composition. The composition is prepared from the following ingredients in parts by weight: 0.1 to 1 part of bacillus velezensis HAB-2 extract, 35 to 55 parts of epsilon-polylysine, 25 to 35 parts of glycine and 4 to 10 parts of alginate oligosaccharide. The preservation number of the bacillus velezensis HAB-2 is CCTCC NO:M 2015070. The invention also discloses a preparation method of the bactericide composition. The bactericide composition disclosed by the invention is wide in disease prevention spectrum and has a remarkable prevention and treatment effect on fungal diseases of various plants.

Description

technical field [0001] The invention relates to the field of pesticides, in particular to a fungicide composition and a preparation method thereof. Background technique [0002] ε-polylysine (ε-PL) is a polymer of L-lysine, and its chemical structure is shown below. ε-polylysine is a light yellow powder with a slightly bitter taste. It is stable to heat and has strong hygroscopicity. It is insoluble in organic solvents such as ether, ethanol, and ethyl acetate, and easily soluble in water. [0003] [0004] ε-polylysine (ε-PL) can directly destroy the selectivity of cells and organelles for the survival of substances and the energy metabolism that depends on the integrity of the membrane structure, causing the rupture of the lysosome membrane in the cell, and then inducing the autolysis of microorganisms. effect, leading to cell death. Existing studies have shown that ε-polylysine has a good inhibitory effect on Escherichia coli, Staphylococcus aureus, Lactobacillus bul...

Claims

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Application Information

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IPC IPC(8): A01N63/22A01N37/46A01N43/16A01N25/22A01P3/00A01P7/00A01P21/00
CPCA01N63/22A01N37/46A01N43/16A01N25/22Y02A50/30
Inventor 刘文波秦春秀缪卫国
Owner HAINAN UNIVERSITY
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