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Culture system and method for promoting growth of photosynthetic organisms

A technology of photosynthetic organisms and culture systems, applied in the cross field of chemistry and biology, can solve the problems of limited growth promotion of photosynthetic organisms, and achieve the effect of improving the utilization rate

Pending Publication Date: 2022-01-14
THE HONG KONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This type of photobioreactor has attracted much attention for producing food, biofuel and reducing carbon dioxide, however, its promotion of the growth of photosynthetic organisms is limited

Method used

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  • Culture system and method for promoting growth of photosynthetic organisms
  • Culture system and method for promoting growth of photosynthetic organisms
  • Culture system and method for promoting growth of photosynthetic organisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Embodiment 1: the synthesis of TPBA:

[0093]

[0094] Under the protection of nitrogen, add tetrakis (triphenylphosphine) palladium [Pd (PPh 3 ) 4 ] and potassium carbonate. After heating to reflux for 20 hours, the mixture was purified to afford TPAP as a white solid. The compound TPAP and N,N-diisopropyl-ethylamine were dissolved in dichloromethane, and then a solution of boron tribromide in dichloromethane was added dropwise on ice. Then, the mixture was stirred overnight at room temperature. The mixture was purified to afford compound TPAPBr as an orange solid. To a solution of the compound TPAPBr in toluene was added diphenylzinc under a nitrogen atmosphere. The mixture was then reacted with stirring at 70°C for 12 hours. Purification of the mixture gave pure TPBA as a yellow solid. 1 H NMR (400MHz, CDCl 3 ), δH=8.44(d, J=5.3Hz, 1H), 7.98-7.95(t, J=7.5Hz, 1H), 7.88(d, J=7.9Hz, 1H), 7.70(d, J=8.3Hz , 1H), 7.49 (s, 1H), 7.30-7.17 (m, 19H), 7.09-7.06 (t, ...

Embodiment 2

[0095] Embodiment 2: the synthesis of APO

[0096]

[0097] Benzohydrazide and 4-(dimethylamino)benzaldehyde were dissolved in methanol, and the resulting mixture was refluxed overnight. Suspend the above-mentioned hydrazone intermediate in 1,2-dichloroethane, and add allyltrimethylsilane and boron trifluoride etherate in sequence. The reaction flask was immersed in a boiling oil bath at 120.1 °C and stirred overnight. The crude product was purified to afford APO as a yellow solid. 1 HNMR(400MHz,THF-d8),d(ppm):8.45–8.43(m,2H),8.16(d,2H,J=7.2Hz),7.86(s,1H),7.59–7.55(m,1H) ,7.51–7.49(m,2H),6.89–6.87(m,2H),3.16(s,6H). 13 C NMR (100MHz, THF-d8), d (ppm): 170.65, 155.63, 151.25, 138.14, 133.58, 129.66, 129.41, 129.27, 128.47, 117.98, 112.88, 112.53, 40.54, 40.22. C 16 h 16 BF 2 N 3 O[M] + The theoretical value of HRMS (MALDI-TOF) is: 316.1354, and the measured value is: 316.1354.

Embodiment 3

[0098] Example 3: Spectral characterization of cyanobacteria and aggregation-induced luminescent molecules in seawater

[0099] In this example, cyanobacteria (CB) and aggregation-induced luminescent molecules APO and TPBA were characterized by spectroscopy, wherein the concentrations of APO and TPBA were 10 -5 M, the concentration of cyanobacteria is 10 6 individual / ml.

[0100] First, the absorption spectrum of cyanobacteria in the visible light range (400nm to 700nm) in seawater (containing 0.1% DMSO) was measured, and the results are as follows: figure 2 As shown in A, the absorption peaks of cyanobacteria are in the blue region of 450nm and the red region of 630nm, respectively, indicating that they have strong absorption in the blue, green and red regions, and weak absorption of light from 550nm to 610nm. In this case, for sunlight in tropical oceans with strong ultraviolet rays (300nm to 400nm), light in the ultraviolet band (300nm to 400nm) cannot be efficiently uti...

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Abstract

The invention relates to a method for promoting growth of photosynthetic organisms, in particular to a method for promoting growth of photosynthetic organisms such as blue-green algae by utilizing aggregation-induced emission molecules. The aggregation-induced emission molecule can form a nano aggregate in a culture medium of photosynthetic organisms, so that short-wavelength light which cannot be absorbed and utilized by the photosynthetic organisms in a light source is absorbed, visible light which can be absorbed and utilized by the photosynthetic organisms is efficiently emitted, and the growth of the photosynthetic organisms is promoted. The invention provides application prospects for carbon dioxide fixation, biological resource development and the like of photosynthetic organisms.

Description

technical field [0001] The present invention relates to the interdisciplinary technical field of chemistry and biology, and in particular relates to a method for promoting the photosynthesis and growth of photosynthetic organisms by using nano-aggregates of aggregation-induced luminescent molecules, providing a solution for the carbon dioxide fixation of photosynthetic organisms and the development of biological resources. application prospects. Background technique [0002] With the growth of the population, the demand for resources is getting higher and higher, and the challenges of global warming, food and sustainable energy shortages are threatening the global population. Therefore, sustainable development is an important goal at present. Photosynthetic organisms convert carbon dioxide and light energy into biomolecules that can be used as food and to produce biofuels. Therefore, large-scale cultivation of photosynthetic organisms provides ideas for providing sustainabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12A01G33/00B01D53/84B01D53/62C12R1/89
CPCC12N1/12A01G33/00B01D53/84B01D53/62Y02A40/80Y02A50/20Y02P20/59Y02P60/20
Inventor 唐本忠刘海翔
Owner THE HONG KONG UNIV OF SCI & TECH
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