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Preparation method of polypeptide hydrogel capable of programmatically releasing NO and promoting angiogenesis

An angiogenesis-promoting and procedural technology, applied in prosthesis, medical science, etc., can solve the problems of increasing cost and reducing application effect, and achieve the effect of high water solubility, good biocompatibility, and easy availability of raw materials

Active Publication Date: 2022-01-18
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the time control is not good, it will greatly reduce its application effect
On the other hand, many different applications require different strengths of hydrogels. Simply increasing the concentration of peptides to increase the strength of hydrogels will increase the cost and bring some negative effects.

Method used

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  • Preparation method of polypeptide hydrogel capable of programmatically releasing NO and promoting angiogenesis
  • Preparation method of polypeptide hydrogel capable of programmatically releasing NO and promoting angiogenesis
  • Preparation method of polypeptide hydrogel capable of programmatically releasing NO and promoting angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method for programmed release of NO-promoting angiogenesis polypeptide hydrogel, comprising the steps of:

[0033] (1) Dissolve sodium carbonate in deionized water, adjust PH=7-10, add gelatin at a ratio of 50g / L, stir and dissolve at 60°C to obtain a gelatin solution.

[0034] (2) Dissolve succinic anhydride in a mixed solution of dimethyl sulfoxide and triethylamine at a ratio of 30g / L; the volume ratio of dimethyl sulfoxide to triethylamine is 25:1.

[0035] (3) Mix the solution obtained in step (1) and step (2) to obtain a mixed solution. The mass ratio of gelatin to succinic anhydride in the mixed solution is 1: 1. After stirring at room temperature for 12 hours, dilute 3 times, and use deionized water in the Dialyze at room temperature for 3 days, change the water every 15 hours, the molecular weight cut-off of the dialysis bag is 8KDa-14KDa, after the dialysis, freeze-dry to obtain the processed gelatin.

[0036] (4) Dissolve the gelatin obtained i...

Embodiment 2

[0040] A preparation method for programmed release of NO to promote angiogenesis polypeptide hydrogel, comprising the following steps:

[0041] (1) Dissolve sodium carbonate in deionized water, adjust PH=7-10, add gelatin at a ratio of 200g / L, stir and dissolve at 45°C to obtain a gelatin solution.

[0042] (2) Dissolve succinic anhydride in a mixed solution of dimethyl sulfoxide and triethylamine at a ratio of 80g / L; the volume ratio of dimethyl sulfoxide to triethylamine is 25:1.

[0043] (3) Mix the solution obtained in step (1) and step (2) to obtain a mixed solution. The mass ratio of gelatin to succinic anhydride in the mixed solution is 1:0.5. After stirring at room temperature for 48 hours, dilute to 2 times, and use deionized water in the Dialyze at room temperature for 5 days, change the water every 18 hours, the molecular weight cut-off of the dialysis bag is 8KDa-14KDa, after the dialysis, freeze-dry to obtain the processed gelatin.

[0044] (4) Dissolve the gelatin...

Embodiment 3

[0048] A preparation method for programmed release of NO-promoting angiogenesis polypeptide hydrogel, comprising the steps of:

[0049] (1) Dissolve sodium carbonate in deionized water, adjust the pH to 7-10, add gelatin at a ratio of 100g / L, stir and dissolve at a temperature of 35°C to obtain a gelatin solution.

[0050] (2) Dissolve succinic anhydride in a mixed solution of dimethyl sulfoxide and triethylamine at a ratio of 60g / L; the volume ratio of dimethyl sulfoxide to triethylamine is 25:1.

[0051] (3) Mix the solution obtained in step (1) and step (2) to obtain a mixed solution. The mass ratio of gelatin to succinic anhydride in the mixed solution is 1:0.1. After stirring at room temperature for 30 hours, dilute to 1 time, and use deionized water in the Dialyze at room temperature for 7 days, change the water every 6 hours, the molecular weight cut-off of the dialysis bag is 8KDa-14KDa, after the dialysis, freeze-dry to obtain the processed gelatin.

[0052] (4) Diss...

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Abstract

The invention discloses a preparation method of polypeptide hydrogel capable of programmatically releasing NO and promoting angiogenesis, and belongs to the field of biological material preparation. The hydrogel disclosed by the invention has the effects of promoting cell adhesion, increasing vascular permeability, denaturation of extracellular matrix, migration and proliferation of vascular endothelial cells, vascularization and the like. The hydrogel is prepared by the following steps of by taking a natural-source high polymer material gelatin as a substrate material, treating the gelatin through succinic anhydride, activating the gelatin by adopting EDC and NHS on the basis, and grafting a vascular endothelial growth factor (VEGF) to a molecular skeleton of the gelatin through covalent linkage. NH3 is partially oxidized to continuously release NO by biosynthetic coupling of the NO cycle with other cycles (e.g., urea cycle); and the hydrogel prepared by the invention has good biocompatibility, can be degraded in vivo, has the effect of promoting vascularization, can actively induce cell adhesion, and has good application prospects in regenerative medicine and clinical treatment.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a method for preparing a polypeptide hydrogel programmed to release NO and promote angiogenesis. [0002] technical background [0003] Hydrogel is a polymer material with a three-dimensional network structure, and the polymer is connected by physical or chemical cross-linking. [0004] It has good biocompatibility, water absorption capacity, degradability and low cytotoxicity. It can swell in aqueous solution and retain a large amount of water without dissolving, so it can maintain water and body fluids, thereby promoting neovascularization and Skin regeneration. [0005] Nitric oxide (NO) is a potent pancreatic stimulator produced by endothelial cells (ECS) and can use ammonia (NH 3 ) production; NO is a vasodilator and promotes EC growth and migration, thereby promoting the sprouting of new blood vessels from existing ones; thus, various NO-releasing biomaterials have re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/52A61L27/54
CPCA61L27/222A61L27/227A61L27/52A61L27/54A61L2300/252A61L2300/414C08L89/00
Inventor 张磊李勇李宁李鹏飞李天晴季维智
Owner KUNMING UNIV OF SCI & TECH
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