Unlock instant, AI-driven research and patent intelligence for your innovation.

In-situ mineralized hydrogel as well as preparation method and application thereof

An in-situ mineralization and hydrogel technology, applied in the fields of medical science, prosthesis, tissue regeneration, etc., can solve problems such as little consideration of natural tissue structure and characteristics, poor integration of surrounding bone tissue, low biological activity, etc., to achieve Excellent biocompatibility, enhance bone healing rate, and promote the effect of repair

Pending Publication Date: 2022-01-18
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such scaffolds are designed with little regard for the structure and characteristics of native tissues, have low bioactivity, and at the same time, scaffolds usually exhibit poor integration with surrounding bone tissue, seriously affecting bone healing.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In-situ mineralized hydrogel as well as preparation method and application thereof
  • In-situ mineralized hydrogel as well as preparation method and application thereof
  • In-situ mineralized hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] a. Add 32.5 mg of hydroxyethylidene diphosphate into 1 mL of sodium hydroxide solution with pH=9.5 at room temperature, fully dissolve, and prepare the solution;

[0092] b. Add 250mg of sodium alginate (Mn=100kDa) into the final solution obtained in step a and stir for 15 minutes to fully dissolve it to prepare a precursor hydrogel;

[0093] c. Prepare 275 mg / mL calcium chloride solution, add 26 μL of calcium chloride solution to the system obtained in step b, stir for 20 minutes to make it evenly mixed;

[0094] d. The system obtained in step c was placed in an oven at 50°C for 24 hours to successfully prepare a hydrogel.

[0095] e. After the final curing, the hydrogel was soaked in the simulated body fluid PBS in vitro at room temperature, and soaked for 10 days to fully perform the ion exchange inside and outside, so as to prepare the biomimetic calcium phosphate hydrogel.

Embodiment 2

[0097] a. Add 16 mg of ibandronate sodium into 2 mL of sodium carbonate-sodium bicarbonate buffer solution (pH=9.4), fully dissolve, and prepare the solution;

[0098] b. Add 360 mg of sodium alginate (Mn=300 kDa) into the final solution obtained in step a and stir for 15 min to fully dissolve it to prepare a precursor hydrogel.

Embodiment 3

[0100] a. Configure 400 mg / mL magnesium chloride solution, add 20 μL of magnesium chloride solution to the system obtained in Example 2, stir fully to make it evenly mixed;

[0101] b. The hydrogel can be successfully prepared by placing the system obtained in step a at 50 degrees Celsius for 0.5 days.

[0102] c. After the final solidification, the hydrogel was soaked in the simulated body fluid PBS in vitro at room temperature, soaked for 10 days to fully perform the ion exchange inside and outside, and prepare the biomimetic magnesium phosphate hydrogel.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Adhesionaaaaaaaaaa
Login to View More

Abstract

The invention provides an in-situ mineralized hydrogel. The in-situ mineralized hydrogel is prepared by blending a degradable high-molecular compound containing carboxylate radical and phosphate radical-containing micromolecules in a solvent under an alkaline condition to prepare a precursor hydrogel, and then cross-linking the precursor hydrogel with metal ions. According to the in-situ mineralized hydrogel as well as the preparation method and application thereof of the invention, the degradable high-molecular compound is used as a framework material, the phosphate radical-containing micromolecules are crystallized and serve as crystal nucleuses to cause deposition and mineralization of metal ions, and electronegativity of the degradable high-molecular compound can accelerate deposition of the metal ions; and finally, co-coordination of carboxylate radicals, phosphate radicals and the metal ions and the crystals of the system form mineralized crystal cross-linked hydrogel. Experimental results show that the mechanical property of the hydrogel prepared by the method of the invention is converted from high toughness to high strength along with the prolonging of time; the hydrogel has relatively high adhesion; an SEM structure recovers small aperture after degradation to form the inorganic mineralized hydrogel; and the hydrogel has low hemolysis rate and cytotoxicity, has excellent biocompatibility, and can effectively promote the proliferation of bone marrow mesenchymal stem cells.

Description

technical field [0001] The invention relates to the technical field of medical polymer materials, in particular to an in-situ mineralized hydrogel and its preparation method and application. Background technique [0002] Bones have a limited ability to regenerate, and defects larger than a certain size cannot heal on their own. In recent years, the medical costs of treating bone-related trauma, infection, and tumors have been increasing. According to relevant statistics, the European market for bone graft substitutes was US$177 million in 2010, and the global orthopedic biomaterials market was valued at US$1.9 billion in the same year, and reached more than US$3 billion in 2017. However, among the top 20 medical device manufacturers in the world, well-known international companies such as Johnson & Johnson, Medtronic, and Braun have used their capital and technological advantages to continuously squeeze the domestic biomedical material market, threatening the safety of my c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08J3/075C08J3/24C08L5/04C08L1/28C08L5/08C08L77/04A61L27/18A61L27/20A61L27/52A61L27/58
CPCC08J3/075C08J3/24A61L27/58A61L27/52A61L27/20A61L27/18C08J2305/04C08J2301/28C08J2305/08C08J2377/04A61L2430/02C08L5/04C08L1/28C08L5/08C08L77/04
Inventor 肖春生刘佳颖陈学思
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI