Fluorescein carbon dot dyeing reagent for fungus detection, dyeing method and application

Abstract

The invention discloses a fluorescein carbon dot dyeing reagent for fungus detection. The fluorescein carbon dot dyeing reagent is prepared from the following components in percentage by mass: 0.01 to 0.1 percent of a fluorescein carbon dot material and 99.90 to 99.99 percent of water, wherein the fluorescein carbon dot material is prepared from the following raw materials in percentage by mass: 1%-2% of a fluorescent whitening agent, 10%-15% of citric acid, 8%-12% of ethylenediamine and 73%-78% of water. The reagent contains the fluorescein carbon dot material, and the fluorescein carbon dot material is stable in fluorescence, high in fluorescence intensity and good in fungus imaging effect. The invention further discloses a dyeing method adopting the fluorescein carbon dot dyeing reagent. The method is simple, easy to implement and short in consumed time. The invention also discloses application of the fluorescein carbon dot staining reagent in fungus detection by adopting a fluorescent staining method.

Description

technical field [0001] The invention belongs to the technical field of fluorescence detection, and in particular relates to a fluorescein carbon dot staining reagent for fungal detection, a staining method and an application. Background technique [0002] In recent years, with the wide application of drugs such as broad-spectrum antibiotics, anti-tumor chemotherapy, and immunosuppressants, the incidence of fungal infections caused by opportunistic fungi has shown an obvious upward trend. However, if fungal infections are not effectively controlled, they may develop For life-threatening systemic diseases and complications. Therefore, accurate and rapid detection of pathogenic fungi is of great significance to the diagnosis and treatment of diseases. [0003] At present, commonly used clinical diagnostic methods include microscopic examination, microbial culture, biochemical methods, and molecular biological diagnostic methods. Among them, the microbial culture method takes ...

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Problems solved by technology

[0009] (1) The fluorescent whitening agent is unstable, easy to photobleach, easy to quench, short bright time, and loses the ability of stimulated emission of fluorescence in an acid environment or a strong alkali environment

[0010] (2) The biocompatibility of fluorescent whitening agents is poor and has certain biological toxicity

[0011] (3) The reagent formula is complicated, the preparation is cumbersome, and the performance is unstable

[0012] (4) The components contain dimethyl sulfoxide (DMSO), which has poor biocompatibility. For example, the patent application with the publication number CN 106467923B is an application of an enhanced fungal staining solution and its preparation method

[0017] (1) There are too many components in the staining solution, which is not easy to store;

[0018] (2) Heating and incubation are required in the sample preparation process, and the operation is cumbersome. For example, the patent application with the publication number CN111019999A is a microbial fluorescent staining solution and its application

Research on carbon dots is limited to staining to identify cellular microorganisms, and there is no related research on staining to identify fungal microorganisms

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