Oxygen-containing benzoic acid in purslane as well as extraction and separation method and application thereof
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A separation method, the technology of purslane, applied in the separation/purification of carboxylic acid esters, food ingredients containing natural extracts, applications, etc., can solve the problem of low structural novelty, and achieve environmentally friendly process methods, simple operation methods and fast effect
Active Publication Date: 2022-01-25
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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[0004]
At present, most of the chemical components isolated from purslane are known, and the structural
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Embodiment 2
[0052] Example 2. Neuroprotective effects of the novel compounds of the present invention.
[0053] 1 main material.
[0054] 1.1 Drugs and reagents: The new oxygen-containing benzoic acid compound used in the experiment was prepared by the above method, with a purity of 95%, weighed accurately, and diluted with DMSO to the required solutions for the following dosage groups. DMEM high glucose medium, fetal bovine serum (Hyclone, USA); penicillin, streptomycin (Hangzhou Sijiqing Bioengineering Materials Co., Ltd.), phosphate buffer saline (PBS, Wuhan Boster Bioengineering Co., Ltd.), ROS detection reagent Box (Shanghai Biyuntian Biotechnology Company).
[0055] 1.2 Cell lines: human neuroblastoma cell lines (SH-SY5Y, IMR-32, Chinese Academy of Sciences Shanghai Cell).
[0056] 1.3 Grouping: divided into control group, H 2 o 2 Damage model group and experimental group, each group.
[0073] Example 3. Anti-acetylcholinesterase effect of the new compounds of the present invention.
[0074] 1 main material.
[0075]1.1 Drugs and reagents: The oxybenzoic acid compound used in the experiment was prepared by the above method with a purity of 97%. Sodium dihydrogen phosphate, disodium hydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.), physostigmine (Shanghai Hanxiang Biotechnology Co., Ltd. company), 5,5′-dithiobis(2-nitrobenzoic acid) (Dithiobisnitrobenzoic acid, DTNB, Shanghai Jinshui Biotechnology Co., Ltd.), acetylcholinesterase (AChE) and iodide thioacetylcholine (Acetylthiocholine iodide, ATCI, Dalian Meilun Biotechnology Co., Ltd).
[0076] 1.2 Grouping: divided into negative control group, positive control group and experimental group.
[0077] 2 Experimental methods.
[0078] 2.1 Sample preparation: Precisely weigh the sample and 1 mg of physostigmine, respectively, and use methanol as the solvent to prepare five gradient concentrations (5 μM...
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Abstract
The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to a novel oxygen-containing benzoic acid compound extracted, separated and identified from purslane and an extraction and separation method thereof. The molecular formula of the novel compound is C14H16O7, and the new compound is named as 4-((4-ethoxy-1,4-dioxobutan-2-yl)oxy)benzoic acid according to the structure of the new compound. The invention also provides an extraction and separation method of the novel compound. The extraction and separation method sequentially adopts ethanol reflux extraction, silica gel column chromatography, polyamide column chromatography, ODS medium-pressure column purification, Bond Elut SI silica gel column chromatography and HPLC for separation, purification and preparation. The structure of the compound is identified as a novel oxygen-containing benzoic acid compound by adopting UHPLC-ESI-QTOF/MS, 1H-NMR, 13C-NMR and two-dimensional nuclear magnetic spectrum analysis methods. The novel oxygen-containing benzoic acid compound has potential neuro-protection activity, acetylcholin esterase resistance activity and the like, and the novel oxygen-containing benzoic acid compound and the salt or the derivative thereof can be used as a synthesis primer of other compounds and a raw material for new drug development and pharmacological activity research. The compound is used for preparing medicines or health-care products for protecting nerves and resisting acetylcholin esterase.
Description
technical field [0001] The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to an oxygen-containing benzoic acid compound extracted, separated and identified from purslane medicinal material and an extraction and separation method thereof. Background technique [0002] purslane( Portulaca oleracea L.), also known as Changshou Cai and Wuxingcao, is an annual herb of the family Portulaca oleracea. Purslane is drought and waterlogging resistant, light and shade tolerant, widely distributed and rich in resources, and has attracted much attention as a wild plant with dual uses for medicine and food. The 2020 edition of "The Pharmacopoeia of the People's Republic of China" records that the dried aerial part of purslane is used as medicine. It has the effects of clearing away heat and detoxification, cooling blood and stopping bleeding, and stopping dysentery. Insect bites, blood in the stool, hemorrhoids and metrorrhagi...
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