Lactobacillus plantarum YL399 capable of producing high-activity tannase and application of lactobacillus plantarum YL399 in preparation of codonopsis pilosula fermented feed

A technology of Lactobacillus plantarum and fermented feed, applied to Lactobacillus plantarum YL399 and its application in the preparation of Codonopsis pilosula fermented feed, can solve the problem of low tannase activity and achieve the effect of expanding the source

Pending Publication Date: 2022-01-28
上海植酵盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the activity of some Lactobacillus plantarum to produce tannase is not high, so the screening of Lactobacillus plantarum with

Method used

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  • Lactobacillus plantarum YL399 capable of producing high-activity tannase and application of lactobacillus plantarum YL399 in preparation of codonopsis pilosula fermented feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Isolation, identification and preservation of embodiment 1 Lactobacillus plantarum strain

[0033] 1. Isolation of Lactobacillus plantarum strains

[0034] Sample material: natural fermented corn silage.

[0035] After repeated streaking culture on the BCP medium plate, pick the single colony that produced the yellow circle and inoculate it on the solid MRS medium plate to continue the culture. Repeated streaking culture and purification on the solid MRS medium plate obtained multiple strains of pure culture strains.

[0036] Inoculate the purely cultured strains into liquid MRS medium for culture, then add 20% glycerol, and store in a -80°C refrigerator.

[0037] Several strains with positive Gram staining, negative catalase test, negative indole reaction, negative hydrogen sulfide production test, negative gelatin liquefaction test, negative hydrolyzed starch test and negative nitrate reduction test were screened out from purely cultured strains. strain. One of the...

Embodiment 2

[0046] The mensuration of the tannase activity produced by embodiment 2 plant lactobacillus (Lactobacillus plantarum) YL399

[0047] 1. Preparation of strain culture and cell-free medium

[0048] The Lactobacillus plantarum (Lactobacillus plantarum) YL399 frozen strain was continuously passaged and activated in liquid MRS medium for 3 times, then inoculated in liquid MRS medium according to the inoculum size of 2%, and cultured statically at 37°C for 16h, 3000r / min , centrifuged at 4°C for 8 minutes, discarded the supernatant, and obtained bacterial precipitates; the obtained bacterial precipitates were ultrasonically disrupted to lyse the cell wall: add an equal volume of sterile normal saline, add lysozyme to a concentration of 1 mg / ml, and bathe in constant temperature water at 37°C for 30 minutes, ice Sonicate the bacterial cells in the bath (800w, 2s, 2s, 40°C, 15min), centrifuge at 4000r / min, 4°C for 10min to collect the supernatant, which is the cell-free extract contai...

Embodiment 3

[0052] Example 3 Application of plant Lactobacillus (Lactobacillus plantarum) YL399 producing high-activity tannase of the present invention in the preparation of Codonopsis pilosula fermented feed

[0053] 1. Preparation of Lactobacillus plantarum YL399 freeze-dried starter

[0054] The frozen Lactobacillus plantarum (Lactobacillus plantarum) YL399 strain was continuously passaged and activated in liquid MRS medium for 3 times, then inoculated into liquid MRS medium at an inoculum size of 2%, cultured at 37°C for 16 h, and centrifuged (3000 ~6000rpm / min, 10min, 4°C) to obtain bacterial cell precipitation, add lyoprotectant (40g / L glucose, 5g / L sodium ascorbate, 80g / L fructooligosaccharide and 30g / L trehalose), freeze-dry, Pack the Lactobacillus plantarum YL399 freeze-dried starter and store it at 4°C. The number of live bacteria of the obtained Lactobacillus plantarum YL399 freeze-dried starter is >100 billion / g.

[0055] 2. Preparation of Codonopsis pilosula fermented feed...

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Abstract

The invention discloses lactobacillus plantarum YL399 capable of producing high-activity tannase and application of the lactobacillus plantarum YL399 in preparation of fermented feed, and relates to the field of fermented feed processing, the lactobacillus plantarum YL399 for producing the high-activity tannase is preserved in the China Center for Type Culture Collection on September 27, 2021, and the preservation number is CCTCC NO: M 20211228. The lactobacillus plantarum YL399 capable of producing high-activity tannase is obtained through separation, identification and screening, the lactobacillus plantarum YL399 is applied to a codonopsis pilosula fermented feed, and experiments prove that the content of tannin in the fermented codonopsis pilosula feed is reduced by 83.92%. According to the invention, the source of tannase is expanded, and a new way is provided for the preparation of microbial fermentation feed.

Description

technical field [0001] The invention relates to the technical field of fermented feed processing, in particular to a strain of Lactobacillus plantarum YL399 producing high-activity tannase and its application in preparing Codonopsis pilosula fermented feed. Background technique [0002] Tannin is a plant secondary metabolite, which is a polyphenolic compound and widely exists in various plant feed materials. Tannins are generally considered nutritionally undesirable, and the presence of higher mass concentrations of tannins in feed has anti-nutritional effects, which will affect the digestion of protein, cellulose, starch and fat by animals, and reduce the nutrition of food or feed value. It is very necessary to take appropriate measures to reduce or remove tannins in feed. [0003] In feed processing, the anti-nutrition of tannins can be reduced or even eliminated by physical degradation, chemical degradation and microbial degradation. Microbial degradation is a long-ter...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23K10/30A23K10/12A23K20/163A23K20/121A23K20/20C12R1/25
CPCC12N1/04C12N1/20A23K10/30A23K10/12A23K20/163A23K20/121A23K20/20A23V2400/169
Inventor 李海权顾振荣
Owner 上海植酵盛生物科技有限公司
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