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Application of fluorescent whitening agent in fungal infection resistance

A fluorescent whitening agent, fungal technology, applied in the application of skin ringworm, the application field of fluorescent whitening agent in inhibiting fungal activity, can solve the problems of difficult to meet drug demand, delay in research and development of new antifungal drugs, etc.

Active Publication Date: 2022-02-08
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, the research and development of new antifungal drugs has been sluggish, and it is difficult to meet the growing demand for drugs. Due to the particularity of dermatophytosis, it is of great significance to discover and develop a new type of topical antifungal drug
[0003] Fluorescent Brightener 85 (Fluorescent Brightener 85, abbreviated as: FB 85, ​​trade name: Fluorescent Brightener VBL) is the leading product of stilbene fluorescent brightener, scientific name 4,4'-bis-(4-light ethyl Amino-anilino-1,3,5-triazin-2-yl)amino-stilbene 2,2'-disulfonic acid sodium salt is widely used in pH chemical sensing materials, chemical sensors, light-emitting diodes, In terms of biological dyeing, etc., it has been in the market for more than 50 years. It is the largest and most widely used fluorescent whitening agent in China. The consumption accounts for more than 85% of the fluorescent whitening agents used in China's detergent industry. As a drug, there is no relevant report on antifungal aspects

Method used

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  • Application of fluorescent whitening agent in fungal infection resistance
  • Application of fluorescent whitening agent in fungal infection resistance
  • Application of fluorescent whitening agent in fungal infection resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Fluorescent whitening agent 85 inhibits fungal activity

[0029] Prepare fluorescent whitening agent 85 with a final concentration of 0.3125 mg / mL-10 mg / mL, and sterilize and filter for later use. The spores of Trichophyton rubrum were washed by adding sterile physiological saline to the plates cultured on Sabouraud dextrose agar medium (SDA) for 7 days to prepare spore suspension. Take an appropriate amount of spore suspension and evenly coat the SDA plate. After the surface of the medium is completely dry, divide it into three areas: the experimental part and the control part, and punch holes with an 8mm hole puncher. White agent 85 was added to the wells, and observed after incubating in a 30°C incubator for 5-7 days. The result is as figure 1 As shown in E and F, fluorescent whitening agent 85 showed strong sensitivity and antibacterial activity to the tested strains Trichophyton rubrum and Microsporum gypsumoides.

[0030] In order to study the potential of fluo...

Embodiment 2

[0032] Inhibitory Effect of Fluorescent Whitening Agent 85 on Drug-resistant Bacteria

[0033] Measure according to the method in Example 1 and obtain that fluorescent whitening agent 85 has bacteriostasis to drug-resistant Candida albicans (Chongqing University of Technology College of Pharmacy and Bioengineering Central Laboratory Preservation), such as figure 2 In A (a ~ b) shown. As the drug concentration increases, its inhibitory effect increases, and the MIC of fluorescent whitening agent 85 against drug-resistant Candida albicans is determined. 80 is 113.28μg / mL, such as figure 2 Middle B.

[0034] Determination of MFC of fluorescent whitening agent 85 against drug-resistant Candida albicans. The drug-resistant Candida albicans strains treated with 78.1 μg / mL fluorescent whitening agent 85, 156.3 μg / mL, and 312.5 μg / mL fluorescent whitening agent 85 solutions were spread on the YPD plate, and the results were as follows figure 2 Shown in C. When the concentratio...

Embodiment 3

[0036] Antifungal mechanism of fluorescent whitening agent 85

[0037] The effect of fluorescent whitening agent 85 on the glucan composition of the cell wall of Trichophyton rubrum was determined by infrared spectroscopy, and the changes of the cell wall of Trichophyton rubrum treated with fluorescent whitening agent 85 were observed by scanning electron microscope. After the fluorescent whitening agent 85 was used to treat Trichophyton rubrum, the bacteria were collected, washed with water, and freeze-dried. The dried cells were suspended in 50 mL of 1 mol / L NaOH solution and reacted at 100 °C for 1 h. Centrifuge at 6000r / min for 15min, discard the supernatant, suspend the precipitate with 50mL of 0.75mol / L NaOH solution, treat at 100°C for 15min, adjust the pH to 4.5 with HCI after cooling, wash twice with water, dehydrate with absolute ethanol and add 20mL After dehydration with ether and air drying, the extracted dextran was pressed into tablets with KBr for red light sp...

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Abstract

The application of the stilbene fluorescent whitening agent provided by the invention in inhibition of fungal activity has excellent antibacterial activity on dermatophytes, trichophyton rubrum, Microsporum gypseum and other fungi, and also has an antibacterial effect on drug-resistant candida albicans. In addition, the invention provides a fluorescent whitening agent 85 / linalool gel preparation which is stable, wide in temperature application range and free of skin irritation and toxicity. The preparation is used for treating a guinea pig model infected by trichophyton rubrum, occurrence and development of skin tinea can be controlled, the antibacterial treatment effect of the gel preparation is superior to that of a positive drug terbinafine in a treatment cycle, and the gel preparation is a potential stable efficient antifungal drug.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of a fluorescent whitening agent in inhibiting fungal activity, especially the application in inhibiting skin ringworm caused by Trichophyton rubrum infection. Background technique [0002] Superficial fungal infection is skin ringworm caused by fungal infections that are close to the cuticle. Common occurrences include tinea corporis, tinea pedis, jock itch, onychomycosis, tinea capitis, and tinea facial, affecting about 20%-25% of the population . According to reports, pathogenic fungi that cause fungal infections in humans are mainly divided into three categories: Trichophyton (Trichophyton genus), Epidermophyton (Epidermophyton genus) and Microsporum (Microsporum genus), among which Trichophyton rubrum ) is the main pathogenic bacteria causing dermatophytosis, accounting for more than 60% of human dermatophytosis. At present, fungal skin diseases cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/53A61K9/06A61K47/32A61K47/10A61P31/10A61P17/00
CPCA61K45/00A61K31/53A61K9/06A61K47/32A61K47/10A61P31/10A61P17/00Y02A50/30
Inventor 王万能成福
Owner CHONGQING UNIV OF TECH
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