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Method and a device for determining the content of a pelteobagrus fulvidraco protease inhibitor based on graphene

A protease inhibitor and assay method technology, applied in the field of yellow catfish component detection, can solve the problems of inability to detect protease inhibitors and their content, inability to meet daily monitoring, inability to ensure food safety, etc., to ensure safety, convenient collection, Easy to use effect

Active Publication Date: 2022-02-08
ZHEJIANG GONGSHANG UNIVERSITY +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, these existing detection methods are still not sensitive enough to detect low levels of protease inhibitors and their content in complex samples, and cannot meet the needs of daily monitoring, thus failing to ensure food safety

Method used

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  • Method and a device for determining the content of a pelteobagrus fulvidraco protease inhibitor based on graphene
  • Method and a device for determining the content of a pelteobagrus fulvidraco protease inhibitor based on graphene
  • Method and a device for determining the content of a pelteobagrus fulvidraco protease inhibitor based on graphene

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experiment example

[0107] Experimental example based on the present invention is as follows:

[0108] Experimental method: take yellow catfish and adopt the present invention to detect.

[0109] Experimental results and analysis:

[0110] 1. Characterization of G / KCC-1

[0111] The morphology and structure of graphite oxide, KCC-1 and G / KCC-1 were studied. KCC-1 was characterized by scanning electron microscopy (SEM), and its images are shown in Figure 4 shown. The outer diameter of the mesoporous KCC-1 nanoparticles is about 500 nm. A wrinkled structure with central radial nano-wrinkles was observed, which significantly enlarged the surface area and increased the ability to absorb target protease inhibitors.

[0112] 2. Effect of G / KCC-1PT-SPE conditions

[0113] In order to obtain an optimal extraction efficiency of protease inhibitors by pipette-tip microextraction, the most influential parameters were investigated, including pH, number of aspiration / dispense cycles for extraction and ...

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Abstract

The invention discloses a method and a device for determining the content of a pelteobagrus fulvidraco protease inhibitor based on graphene. The determination method comprises the following steps: preparation of a sample extract, preparation of a G / KCC-1 composite material, G / KCC-1PT-SPE, UPLC-MS / MS analysis and data statistical analysis. The measuring device comprises a liquid chromatograph-mass spectrometer and a chromatographic column fixing bracket. According to the method for determining the content of the yellow catfish protease inhibitor based on the graphene, the protease inhibitor in the pelteobagrus fulvidraco can be separated and quantitatively determined in a high-selectivity, sensitive and accurate mode, and the G / KCC-1 has the advantages of being small in adsorbent dosage, small in organic solvent dosage, easy and convenient to operate, large in adsorption capacity and good in reproducibility and is is an effective adsorbent for separating and enriching the protease inhibitor in a complex sample, the sample amount and consumption amount required by detection by adopting the method are obviously lower than those of the existing method, food quality monitoring on the protease inhibitor in fish or fish products is facilitated, and the food safety is ensured.

Description

technical field [0001] The invention relates to the field of detection of yellow catfish components, in particular to a method and device for measuring the content of yellow catfish protease inhibitors based on graphene. Background technique [0002] Yellow catfish (Pelteobagrus fulvidraco), belonging to the family Silyformes and the blue catfish family, is a commercially important aquaculture fish that is favored by Asian consumers. In China, yellow catfish farming has become an emerging industry, with an annual output of 480,000 tons in 2018. Due to its excellent meat sensory quality and rich nutrition, yellow catfish farming is showing a continuous growth trend. According to reports, yellow catfish is rich in crude protein, mineral elements, polyunsaturated fat, vitamin E, and has a high proportion of essential amino acids. However, the increase in domestic and foreign market demand has prompted yellow catfish to be cultured under intensive conditions (about 3000kg / 667m2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634Y02A50/30
Inventor 沈清李运燕颜凯陈康朱小芳赵巧灵王萍亚
Owner ZHEJIANG GONGSHANG UNIVERSITY
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