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Composition for inhibiting helicobacter pylori and application thereof

An anti-Helicobacter pylori technology and a composition are applied in the field of compositions for inhibiting Helicobacter pylori, can solve problems such as large side effects, high recurrence rate, antibiotic resistance, etc., and achieve the effects of being conducive to large-scale industrial production and high inhibition rate

Pending Publication Date: 2022-02-22
江苏安泰生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, diseases caused by H. pylori infection are mainly treated with the triple method, that is, proton pump inhibitors plus two antibiotics for antibacterial treatment, but this method has defects such as antibiotic resistance, large side effects, and high recurrence rate

Method used

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  • Composition for inhibiting helicobacter pylori and application thereof
  • Composition for inhibiting helicobacter pylori and application thereof
  • Composition for inhibiting helicobacter pylori and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048]The preparation of embodiment 1 egg yolk protein

[0049] Select 20-week-old hens, mix and fully emulsify the monoepitope antigen peptide shown in SEQ ID NO: 2 with Freund's complete adjuvant in equal volumes, and treat the breast muscle and thigh root of the hen at a dose of 1ml / hen Multi-point injection; every two weeks, use the monoepitopic antigen peptide or dendritic epitope peptide and Freund's incomplete adjuvant to mix equal volumes and fully emulsify the immunogen to boost the immunization once, the immunization dose is 1ml / body, A total of 3 booster immunizations were performed; eggs were collected 2 weeks after the last booster immunization, and unimmunized eggs were collected as negative controls.

[0050] Remove the sterilized eggs, collect the yolk and puncture it, measure 10ml egg yolk liquid, add 10 times of double distilled water to dilute, mix well, adjust the pH value to 4-5, overnight at -20°C, thaw at room temperature, 12000rpm at 4°C Centrifuge for...

Embodiment 2

[0051] The preparation of embodiment 2 barley tender seedling extract

[0052] After cleaning and draining the fresh barley seedlings, use a vacuum freeze dryer for freeze-drying, the pre-freezing temperature is -45°C, the cold trap temperature is -37°C, the sublimation temperature is 25°C, and the vacuum degree is 170Pa; Mesh sieve; take 5.0g sample and place it in a 250ml conical flask, add 95% (v / v) ethanol solution to extract, the ratio of solid to liquid is 25:1, seal the conical flask with a sealing film and place it in a shaker In the bed, extract at 37°C and 100rpm for 60min; after the extraction is completed, centrifuge the extract at 10,000rpm at 4°C for 10min, and take the supernatant to obtain the product.

Embodiment 3

[0053] The preparation of embodiment 3 lactoferrin

[0054] 1) Raw milk pretreatment: filter the raw milk with sterile gauze to remove most impurities, purify it with a centrifugal milk cleaner, cool it in a pool, and store it at -18°C for use;

[0055] 2) Slow down: place the frozen colostrum at room temperature to slow down when thawing;

[0056] 3) degreasing: degreasing with a cream separator;

[0057] 4) Precipitation of casein: the skim milk is placed at 38°C and pH 4.6 for precipitation;

[0058] 5) Whey separation: the filter membrane is made of cellulose acetate and polysulfone ultrafiltration membrane, first treated with a membrane with a molecular weight cut-off of 60,000, when the permeate reaches 0.5L, add the same volume of normal saline to the concentrated solution, and then process Get 0.25L molecular weight greater than 8×10 5 The filtrate, the resulting concentrated solution is treated with a membrane with a molecular weight cut-off of 100,000 to obtain 0....

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Abstract

The invention provides an anti-helicobacter pylori composition prepared from a high-specificity egg yolk antibody and animal and plant extracts, wherein the high-specificity egg yolk antibody is obtained from SEA-MAP immune poultry animals prepared from a helicobacter pylori single-epitope antigen peptide. The inhibition rate on helicobacter pylori is high, and large-scale industrial production is facilitated; and a new means is provided for prevention and treatment of helicobacter pylori infection related diseases.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a composition for inhibiting Helicobacter pylori and its application. Background technique [0002] Helicobacter pylori (H. pylori) is a Gram-negative bacillus with a high infection rate, which can cause gastritis, gastric ulcer and even gastric cancer. Only a small number of infected persons will develop clinical symptoms, while most asymptomatic carriers are often ignored, which indirectly leads to the widespread transmission of Helicobacter pylori among the population. So far, the diseases caused by H. pylori infection are mainly treated with the triple method, that is, proton pump inhibitors plus two antibiotics for antibacterial treatment, but this method has defects such as antibiotic resistance, large side effects, and high recurrence rate. Therefore, it is necessary to develop new methods for the prevention and treatment of H. pylori infection. [0003] Yolk ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/40A61P31/04C07K16/12C07K16/02A61K38/40A61K36/8998
CPCA61K39/40A61K38/40A61K36/8998A61P31/04C07K16/1203C07K16/02A61K2300/00
Inventor 闫小君罗进
Owner 江苏安泰生物技术有限公司
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