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Conotoxin and collagen fusion protein as well as preparation method and application thereof

A technology of fusion protein and collagen, applied in the field of genetic engineering, can solve the problems of high cytotoxicity, weak wrinkle-removing and moisturizing ability of conotoxin, low biological safety of raw materials, etc., so as to reduce cytotoxicity and increase transdermal absorption capacity. , the effect of improving biosafety

Pending Publication Date: 2022-03-01
西安德诺海思医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the currently commercially available conotoxins have high cytotoxicity, resulting in low biological safety of their raw materials, and the wrinkle removal and moisturizing abilities of conotoxins are generally weak

Method used

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  • Conotoxin and collagen fusion protein as well as preparation method and application thereof
  • Conotoxin and collagen fusion protein as well as preparation method and application thereof
  • Conotoxin and collagen fusion protein as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0053] 1. Fusion protein molecular design

[0054] 1. Type 1 collagen (type Ⅲ), family conotoxin protein sequence and gene sequence can be found on the NCBI website.

[0055] The amino acid sequence of conotoxin is shown in SEQ.ID.NO.1;

[0056] The amino acid sequence of human-like collagen is shown in SEQ.ID.NO.2;

[0057] The amino acid sequence of the transdermal peptide is shown in SEQ.ID.NO.3;

[0058] The amino acid sequence of the connecting peptide is shown in SEQ.ID.NO.4;

[0059] See SEQ.ID.NO.5 for the amino acid sequence of the fusion protein.

[0060] The SWISS-MODEL modeling 3D structure of the fusion protein molecule of this embodiment is as follows figure 1 shown.

[0061] 1.2 According to the codon preference of Pichia pastoris, artificially design the nucleotide sequence of the fusion protein, add enzyme cutting sites directly at both ends of the sequence, and insert it into the vector directly after chemical synthesis. The final determined nucleotide ...

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Abstract

The invention discloses conotoxin and collagen fusion protein as well as a preparation method and application thereof, and relates to the technical field of biological genes. The amino acid sequence of the fusion protein is as shown in SEQ. ID. No.5. The fusion protein disclosed by the invention is proved to be capable of effectively reducing the cytotoxicity of the conotoxin and improving the biological safety of raw materials on the premise of not influencing the effect of the conotoxin by collagen fusion; meanwhile, transdermal peptide is added during molecular design, the wrinkle removing and moisturizing capacities are verified by efficacy, and it is proved that the transdermal absorption capacity of fusion protein can be effectively improved by adding the transdermal peptide, and transdermal absorption of conotoxin is promoted.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a conotoxin-collagen fusion protein and a preparation method and application thereof. Background technique [0002] Nicotinic acetylcholine receptors (nicotinic acetylcholinereceptors.nAChRs) are the first class of receptors discovered. It is a ligand-gated ion channel protein that mediates rapid signal transmission between synapses and is widely distributed in the central and peripheral nervous systems and muscles. There are two types of nAChR: muscle type and nerve type. It is a transmembrane pentamer composed of 5 subunits. Muscle type nAChR is commonly found in the plasma membrane of vertebrate skeletal muscle cells and some fish discharge organ cells. The receptor binds to acetylcholine, causing Na + The opening of the channel, Na + Influx into target cells depolarizes the plasma membrane and causes contraction of the cells. It is divided into two categories:...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62C12N15/81C12N1/19A61K8/65A61Q19/00A61Q19/08C12R1/84
CPCC07K14/78C12N15/815A61K8/65A61Q19/08A61Q19/00C07K2319/55
Inventor 魏文培郝东周浩赵硕文侯增淼
Owner 西安德诺海思医疗科技有限公司
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