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Bacillus coagulans and application thereof in treatment of alcoholic liver disease

A technology for Bacillus coagulans and alcoholic liver disease, applied in the field of microorganisms, can solve problems such as Bacillus coagulans alcoholic liver disease, and achieve the effects of reducing lipid accumulation, repairing liver tissue, and repairing intestinal barrier

Active Publication Date: 2022-03-01
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no relevant report on the treatment of alcoholic liver disease with Bacillus coagulans

Method used

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  • Bacillus coagulans and application thereof in treatment of alcoholic liver disease
  • Bacillus coagulans and application thereof in treatment of alcoholic liver disease
  • Bacillus coagulans and application thereof in treatment of alcoholic liver disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Isolation, Identification and Preservation of Example 1 Bacterial Strains

[0055] 1. Isolation of strains

[0056] Samples were collected from a cattle farm in Xinkai Village, Mianzhu City, Deyang City, Sichuan Province. The samples taken were fresh cow feces.

[0057] The samples were streaked repeatedly on the solid YPD medium plate, and the single colony that produced the yellow circle was picked and inoculated on the solid YPD medium plate to continue the culture, and then repeatedly streaked and purified on the solid YPD medium plate to obtain multiple pure culture strains .

[0058] Gram-positive strains were screened from purely cultured strains and inoculated into liquid YPD medium for culture, then added 20% glycerol, and stored in a -80°C refrigerator.

[0059] 2. Identification of strains

[0060] Morphological identification, physiological and biochemical identification and molecular identification were performed on the isolated strains, and the strain F...

Embodiment 2

[0068] Example 2 Therapeutic Effect of Bacillus coagulans FCYS01 on Alcoholic Liver Disease Mice

[0069] 1. Preparation of bacterial suspension

[0070] The preparation method of the bacterial suspension: suspend the tested bacteria with PBS buffer, so that the bacterial concentration is 1.0×10 9 CFU / 200 μL.

[0071] The test bacteria is Bacillus coagulans FCYS01, and the obtained bacterial suspension is named as FCYS01 bacterial suspension.

[0072] Two, group processing

[0073] 45 male C57 mice were randomly divided into 3 groups, 15 in each group, and the grouping was handled as follows:

[0074] Blank group: from the beginning to the end of the test, the Lieber-DeCarli basic control liquid feed was used to feed; from the 8th day of the test to the 15th day, sterile PBS buffer solution was given (administration method: gavage, dosage: 200ul / per only / every day); on the 16th day, give 45% (w / v) dextrin solution (administration mode: gavage, administration amount: the vo...

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PUM

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Abstract

The invention discloses bacillus coagulans and application thereof in treatment of alcoholic liver diseases, and belongs to the field of microorganisms. The invention provides application of bacillus coagulans FCYS01 in preparation of drugs for preventing and / or treating alcoholic liver diseases, the bacillus coagulans FCYS01 is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20211273, the preservation address is Wuhan University, Wuhan, China, the preservation date is October 14, 2021, and the preservation number is CCTCC NO: M 20211273. Experiments prove that the bacillus coagulans FCYS01 reduces lipid accumulation by reducing expression of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase in serum of an alcoholic liver mouse and expression of proinflammatory factors and triglyceride in liver tissues, improves expression of anti-inflammatory factors and intestinal connexin in the liver tissues of the alcoholic liver mouse, repairs intestinal barriers, and improves the liver function of the alcoholic liver mouse. Therefore, the alcoholic liver disease induced by alcohol is prevented and treated.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacillus coagulans and its application in the treatment of alcoholic liver disease. Background technique [0002] Chronic alcohol abuse is a leading cause of liver-related disease and consequent death. The World Health Organization estimates in the World Report on Alcohol and Health that there were approximately 3 million deaths from alcoholic disorders in 2016, accounting for 5.1% of global disease. Continuous alcohol consumption can lead to a variety of chronic alcoholic liver diseases (ALD), including alcoholic steatohepatitis (ASH), alcoholic fatty liver (AFL), alcoholic fibrosis (AF), alcoholic hepatitis (AH), alcoholic liver cirrhosis (AC) and hepatocellular carcinoma (HCC). ALD can progress gradually to ASH and eventually to HCC, leading to death. At present, the treatment of ALD mainly focuses on phosphodiesterase inhibitors, glucocorticoids, polyene phosphatidylcholine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A61P1/16C12R1/07
CPCA61K35/742A61P1/16
Inventor 彭楠刘真真刘通范玉蓉张贞婷
Owner HUAZHONG AGRI UNIV