Self-oxygen-supply targeted bionic nuclear membrane structure nano-composite as well as preparation method and application thereof

A nanocomposite, self-oxygenating technology, applied in nanomedicine, nanotechnology, nanotechnology, etc., can solve problems such as limiting the curative effect of tumor treatment, achieve enhanced curative effect of tumor treatment, good reproducibility, and economical and easy-to-obtain raw materials Effect

Pending Publication Date: 2022-03-15
宁波大学附属人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tumor hypoxia can lead to various treatment resistances, which greatly limits the efficacy of tumor therapy. Therefore, finding a suitable strategy to improve tumor hypoxia is crucial to improve the efficacy of tumor therapy

Method used

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  • Self-oxygen-supply targeted bionic nuclear membrane structure nano-composite as well as preparation method and application thereof
  • Self-oxygen-supply targeted bionic nuclear membrane structure nano-composite as well as preparation method and application thereof
  • Self-oxygen-supply targeted bionic nuclear membrane structure nano-composite as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Synthesize self-oxygen-targeted biomimetic nuclear membrane structure nanocomposites according to the following steps:

[0077] S1. Preparation of ICG-loaded biomimetic liposomes: take lecithin, DSPE-PEG2000, cholesterol, and ICG-ODA in a molar ratio of 100:20:4:10, dissolve them in chloroform solution and mix them thoroughly, and then rotary evaporate at 40°C to form a film. Vacuumize the film for 3 hours to completely volatilize the organic solvent; add PBS to the round bottom flask after film formation, hydrate under ultrasonic conditions at 60°C, and obtain ICG-loaded liposomes after centrifugation with deionized water; the obtained ICG-loaded liposomes The plastid and the extracted 4T1 tumor cell membrane protein were co-extruded with a 400nm polycarbonate membrane in an ice bath using a liposome extruder to obtain ICG-loaded biomimetic liposomes, washed by centrifugation, and stored at 4°C.

[0078] S2. Preparation of monodisperse SiO by sol-gel method 2 Nanosphe...

Embodiment 2

[0081] S1. Preparation of ICG-loaded biomimetic liposomes: take lecithin, DSPE-PEG2000, cholesterol, and ICG-ODA in a molar ratio of 100:20:4:10, dissolve them in chloroform solution and mix them thoroughly, and then rotary evaporate at 40°C to form a film. Vacuumize the film for 3 hours to completely volatilize the organic solvent; add 5% sucrose to the round-bottomed flask after film formation, hydrate under ultrasonic conditions at 55 ° C, and obtain ICG-loaded liposomes after centrifugal cleaning with deionized water; ICG liposomes and extracted 4T1 tumor cell membrane proteins were co-extruded with a 400nm polycarbonate membrane in an ice bath using a liposome extruder to obtain ICG-loaded biomimetic liposomes, cleaned by centrifugation, and freeze-dried for storage.

[0082] S2. Preparation of monodisperse SiO by sol-gel method 2 Nanospheres, washed with ethanol / water solution, centrifuged at room temperature, added KMnO under ultrasonic conditions 4 , continue to sonic...

Embodiment 3

[0085] Follow the steps below to synthesize self-oxygen-targeted biomimetic nuclear membrane nanocomposites

[0086] S1. Preparation of ICG-loaded biomimetic liposomes: take lecithin, DSPE-PEG2000, cholesterol, and ICG-ODA in a molar ratio of 100:20:4:10, dissolve them in chloroform solution and mix them thoroughly, and then rotary evaporate at 40°C to form a film. Vacuumize the film for 3 hours to completely volatilize the organic solvent; add Tris solution to the round-bottomed flask after film formation, hydrate under ultrasonic conditions at 55°C, and obtain ICG-loaded liposomes after centrifugation with deionized water; the obtained ICG-loaded liposomes Liposomes and extracted 4T1 tumor cell membrane proteins were co-extruded with a 400nm polycarbonate membrane using a liposome extruder to obtain ICG-loaded biomimetic liposomes, washed by centrifugation, and stored at 4°C.

[0087] S2. Preparation of monodisperse SiO by sol-gel method 2 Nanospheres, washed with ethanol / w...

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Abstract

The invention provides a self-oxygen-supply targeting type bionic nuclear membrane structure nano-composite as well as a preparation method and application thereof. The nano-composite comprises a bionic lipid membrane and a hollow MnO2 nuclear membrane structure, the bionic lipid membrane is a phospholipid bimolecular membrane modified by tumor cell membrane protein on the surface; the hollow MnO2 is used for loading a tumor therapeutic drug, and the drug is at least one of a chemotherapeutic drug and an immunotherapeutic drug; one of the purposes of the invention is to provide the self-oxygen-supply targeting type bionic nuclear membrane structure nano-composite which can be used for magnetic resonance imaging, fluorescence imaging, tumor chemotherapy and optical therapy; the second purpose of the invention is to provide a preparation method of the self-oxygen-supply targeting type bionic nuclear membrane structure nano-composite.

Description

technical field [0001] The invention relates to the technical field of drug carriers, in particular to a self-oxygen-supply targeting biomimetic nuclear membrane structure nanocomposite and its preparation method and application. Background technique [0002] The traditional nanomedicine targeting strategy is divided into two steps: first, the surface of nanoparticles is modified with polyethylene glycol to avoid clearance by the reticuloendothelial system; second, ligand modification is performed to obtain targeting capabilities. However, recent studies have shown that nano-drugs modified by polyethylene glycol will be quickly cleared by the liver after continuous administration, and the ligand modification technology is cumbersome, and there are problems such as insufficient modification breadth. Therefore, the actual targeting effect in vivo More often than not. [0003] The tumor hypoxic microenvironment is closely related to the occurrence and development of tumors, an...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/64A61K41/00A61K45/06A61K49/00A61K49/14A61K49/18A61P35/00B82Y5/00B82Y20/00B82Y30/00B82Y40/00
CPCA61K47/6923A61K47/6911A61K47/64A61K41/0052A61K41/0057A61K45/06A61K49/1812A61K49/14A61K49/0056A61K49/0084A61P35/00B82Y5/00B82Y20/00B82Y30/00B82Y40/00A61K2300/00
Inventor 伍满香李强陈天翔吴爱国王联芙李建斌史燕巧房倩兰
Owner 宁波大学附属人民医院
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