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Application of 2-amino-3-phenylbutyric acid or 2, 6-diamino-3-methylhexanoic acid as plant immune resistance inducer

An immune inducer, phenylbutyric acid technology, applied in the application field of plant immune inducer, can solve the problems that do not involve the research, report and patent of natural products and plant immune inducer activity, and achieve simple biological extraction , the effect of simple structure

Active Publication Date: 2022-03-18
NANJING TIANNONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are few studies on 2,6-diamino-3-methylhexanoic acid, and there are no related studies, reports and patents involving natural products and plant immune induction activities

Method used

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  • Application of 2-amino-3-phenylbutyric acid or 2, 6-diamino-3-methylhexanoic acid as plant immune resistance inducer
  • Application of 2-amino-3-phenylbutyric acid or 2, 6-diamino-3-methylhexanoic acid as plant immune resistance inducer
  • Application of 2-amino-3-phenylbutyric acid or 2, 6-diamino-3-methylhexanoic acid as plant immune resistance inducer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 (biological synthesis, extraction method and structural identification of the compound of the present invention)

[0052] (1) Cultivation of Alternaria

[0053] Glucose sodium nitrate medium: glucose, 40.0g; NaNO 3 , 1.0g; NH 4 Cl, 0.25g; KH 2 PO 4 , 1.0g; KCl, 0.25g; NaCl, 0.25g; MgSO 4 ·7H 2 O, 0.5g; FeSO 4 ·7H 2 O, 0.01g; ZnSO 4 ·7H 2 O, 0.01g; yeast extract, 1g, add water to 1L, adjust pH to 5.5.

[0054] The Alternaria culture method is as follows: PDA medium activates the preserved strains, and after 7 days, select colonies with consistent growth, take a 5mm diameter bacterial cake, and inoculate it into 500mL medium, and the inoculation amount is one bacterial cake per 100mL. Place the medium inoculated with the bacterial blocks into a constant temperature shaker, and the culture conditions are: 140 rpm, 25°C, and dark culture for 7 days.

[0055] (2) Extraction of compounds

[0056] The mycelia were separated from the fermentation broth ...

Embodiment 2

[0069] Example 2 (2-amino-3-phenylbutyric acid and 2,6-diamino-3-methylhexanoic acid induce tobacco resistance to tomato spotted wilt virus infection)

[0070] Tomato spotted wilt virus was obtained from Yunnan Province, China. The initial source of the virus was stored in a -80°C refrigerator. The friction inoculation method was used to inoculate it on the leaves of Nicotiana benthamiana to activate the virus. The virus plasmid was extracted and transformed into Escherichia coli competent cells. , smeared on a resistant plate and cultured, picked a single colony for PCR screening, selected positive colonies for sequencing and subsequent plasmid extraction, added the plasmids with normal sequencing to Agrobacterium competent cells, and used the electric shock method for Agrobacterium For transformation, the transformed Agrobacterium liquid was spread on the corresponding resistance screening plate, and cultured at 28°C (±1) for 48h. Pick a single colony of Agrobacterium on the...

Embodiment 3

[0088] Example 3 (2-amino-3-phenylbutyric acid and 2,6-diamino-3-methylhexanoic acid induce wheat resistance to powdery mildew infection)

[0089] 2-Amino-3-phenylbutyric acid was dissolved in distilled water and then gradually diluted with distilled water to form 10nM, 100nM, 1000nM and 10000nM solutions, and a blank control was also set up. Wheat (NAU0686) seeds were germinated, planted in sterilized soil cultivation pots, and placed in a greenhouse at 23(±1)°C for 12h under light for cultivation. When the seedling grows to 1 leaf and 1 heart stage: the wheat seedlings are sprayed with stems and leaves with the 2-amino-3-phenylbutyric acid solution of the above-mentioned concentration, and the treatment is repeated once in 24 hours at intervals, and two treatments are carried out altogether. After 24 hours, fresh powdery mildew spores were evenly sprinkled on the wheat leaves, with 3 pots per treatment, 20 plants per pot. After 10 days, investigate the wheat disease level o...

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Abstract

The invention discloses an application of 2-amino-3-phenylbutyric acid or 2, 6-diamino-3-methylhexanoic acid serving as a plant immune resistance inducer. 2-amino-3-phenylbutyric acid and / or 2, 6-diamino-3-methylhexanoic acid are / is used as natural active substances to be developed into the plant immune resistance inducer which can be used for improving the resistance of plants to biological stress and abiotic stress, and infection of fungi, viruses and bacteria on the plants and reduction of the pathogenic level are effectively prevented; meanwhile, the tolerance of plants to high temperature, low temperature, drought and salt stress can be remarkably improved. The 2-amino-3-phenylbutyric acid and the 2, 6-diamino-3-methylhexanoic acid have the characteristics of safety, environmental protection and high efficiency.

Description

technical field [0001] The invention belongs to the field of agricultural biological pesticides and relates to the application of 2-amino-3-phenylbutyric acid and / or 2,6-diamino-3-methylhexanoic acid as a plant immune inducer. Background technique [0002] In recent years, extreme weather has occurred frequently around the world, and the abiotic stresses faced by agricultural plants have become increasingly serious. Every year, major abiotic stresses such as high temperature, low temperature, drought and salt cause huge losses to agricultural production. Drought is one of the most important adversity stress factors affecting the survival, growth and distribution of plants. At present, the global arid and semi-arid areas account for more than 40% of the total cultivated land area. High temperature and low temperature seriously affect the growth and development of plants, thereby affecting the yield and quality of plants. In recent years, due to the deterioration of the glob...

Claims

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Application Information

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IPC IPC(8): A01N37/44A01N25/30A01P21/00A01P1/00A01P3/00
CPCA01N37/44A01N25/30A01P21/00A01P1/00A01P3/00Y02A40/10
Inventor 陈世国王赫房婉萍王良省郭爱平
Owner NANJING TIANNONG BIOTECHNOLOGY CO LTD
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