Preparation method of polypyrrole modified bovine serum albumin, protein conductive hydrogel as well as preparation method and application of protein conductive hydrogel
A bovine serum albumin and conductive hydrogel technology, applied in the field of protein conductive hydrogel and its preparation, can solve the problems of only reaching tensile stress, poor mechanical properties, poor mechanical strength, etc., so as to improve mechanical strength and strengthen mutual effect, the effect of improving conductivity
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Embodiment 1
[0033] A method for preparing a protein conductive hydrogel, the steps comprising:
[0034] (1) Preparation of polypyrrole-modified bovine serum albumin
[0035] Weigh 10g of bovine serum albumin solution in 100mL (0.25mol / L, pH=9) of CO 3 2- / HCO 3 - In the buffer solution, fully magnetically stir under ice bath conditions to form a uniform solution; then, add 2 mL of methacrylic anhydride dropwise to the flask, continue to stir and react for 1 h under ice bath conditions, and distill the resulting solution with molecular water Dialyzed for 48 hours, then obtained alkenylated bovine serum albumin by freeze-drying;
[0036] Weigh 2 g of alkenylated bovine serum albumin and dissolve it in 100 mL (0.2 mol / L, pH=7.4) PBS buffer solution. After fully stirring to dissolve completely, add 1.2 mL of monomeric pyrrole drop by drop, and stir at room temperature for 2 h. , slowly add 0.4g initiator ammonium persulfate to the mixed solution, continue to stir for 24h, after the react...
Embodiment 2-6
[0048] Examples 2-6 provide the preparation method of protein conductive hydrogel, which is basically the same as Example 1. The difference from Example 1 is that the amount of polypyrrole-modified bovine serum albumin is different. Please refer to Table 1 for details.
[0049] The test result of the consumption of the different polypyrrole modified bovine serum albumin of table 1
[0050] test group polypyrrole modified bovine serum albumin Fracture stress elongation at break Example 2 0 2.52MPa 640％ Example 3 0.03g 3.77MPa 632％ Example 4 0.06g 4.81MPa 604％ Example 1 0.09g 5.4MPa 570％ Example 5 0.12g 5.29 MPa 504％ Example 6 0.15g 4.64 MPa 380％
[0051] As can be seen from the data in Table 1, as the polypyrrole-modified bovine serum albumin content increases from 0.09g, the breaking stress increases gradually, and the elongation at break gradually decreases; continue to increase the polypyrrole-modified ...
Embodiment 7-9
[0053] Examples 7-9 provide the preparation method of protein conductive hydrogel, which is basically the same as Example 1, except that the concentration of ferric ions is different from Example 1, please refer to Table 2 for details.
[0054] The test result of the concentration of different ferric ions in table 2
[0055] test group The concentration of ferric ions Fracture stress elongation at break Example 7 0.06mol / L 3.45MPa 650％ Example 8 0.08mol / L 4.57MPa 603％ Example 1 0.1mol / L 5.4MPa 570％ Example 9 0.12gmol / L 4.90MPa 500％
[0056] As can be seen from the data in Table 2, as the concentration of soaked ferric ions increases from 0.06mol / L to 0.1mol / L, the fracture stress increases and the elongation at break decreases; continue to increase the concentration of ferric ions to 0.12mol / L, The stress at break decreases and the elongation at break also decreases.
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