Compound microecological preparation for breeding stichopus japonicus and inhibiting pathogenic vibrio

A composite microecological, pathogenic Vibrio technology, applied in the direction of microorganisms, microorganism-based methods, bacteria, etc.

Active Publication Date: 2022-03-25
LIAONING OCEAN & FISHERIES SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, so far there is no relevant report on the compound microecological preparations for cultured sea cucumbers that inhibit pathogeni

Method used

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  • Compound microecological preparation for breeding stichopus japonicus and inhibiting pathogenic vibrio
  • Compound microecological preparation for breeding stichopus japonicus and inhibiting pathogenic vibrio
  • Compound microecological preparation for breeding stichopus japonicus and inhibiting pathogenic vibrio

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of compound microecological preparation for cultivating sea cucumbers of the present invention to inhibit pathogenic Vibrio is composed of Rhodopseudomonas palustris ( Rhodopseudomonas palustris ), Clostridium butyricum ( Clostridium butyricum ), eucalyptus leaf extract and aloe vera leaf extract are mixed as feed additives, and Rhodopseudomonas palustris 10 per gram of feed is added 5 cfu, Clostridium butyricum 10 7 cfu, oak leaf extract 1 mg, aloe vera leaf extract 4 mg;

[0028] The eucalyptus leaf extract and aloe vera leaf extract are prepared according to the following method: the purchased eucalyptus leaves and aloe vera leaves are cleaned with distilled water, and dried in the shade at 25°C; , adding an ethanol solution with a volume fraction of 65%, and a solid-liquid ratio of 0.05 g / mL; leaching at room temperature for 1 h under the action of ultrasonic waves with a working frequency of 40 KHZ and a power of 80 W; put the leaching solution into an u...

Embodiment 2

[0038] The preparation process of Rhodopseudomonas palustris, Clostridium butyricum, oak leaf extract and aloe vera leaf extract is basically the same as in Example 1. The difference is that the cultivation temperature of Rhodopseudomonas palustris is 35 ℃, and the light intensity is 3000 lux. The volume fraction of ethanol extraction solution of oak leaf extract and aloe vera leaf extract was 85%, the solid-liquid ratio was 0.1 g / mL, and the extraction time was 2.5 h. Add Rhodopseudomonas palustris 10 per gram of feed 7 cfu, Clostridium butyricum 10 5 cfu, oak leaf extract 2 mg, aloe vera leaf extract 8 mg;

Embodiment 3

[0040] The preparation process of Rhodopseudomonas palustris, Clostridium butyricum, oak leaf extract and aloe vera leaf extract is basically the same as in Example 1. The difference is that the culture temperature of Rhodopseudomonas palustris is 33 ℃, and the light intensity is 2500 lux. The volume fraction of ethanol extraction solution of oak leaf extract and aloe vera leaf extract was 75%, the solid-liquid ratio was 0.07 g / mL, and the extraction time was 2 hours. Add Rhodopseudomonas palustris 10 per gram of feed 6 cfu, Clostridium butyricum 10 6 cfu, oak leaf extract 1.5 mg, aloe vera leaf extract 6 mg;

[0041] The colony morphology of Rhodopseudomonas palustris and Clostridium butyricum in embodiment 3 is as follows figure 1 As shown, Rhodopseudomonas palustris (A) is a maroon round colony with neat and smooth edges and a colony diameter of 0.3 mm to 0.60 mm; Clostridium butyricum (B) is a milky white round colony with a colony surface Smooth, raised, neat edges, ...

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Abstract

The invention discloses a compound microecological preparation for inhibiting pathogenic vibrio for breeding stichopus japonicus, which is prepared by mixing rhodopseudomonas palustris, clostridium butyricum, oak leaf extract and aloe leaf extract into a feed additive, and the addition amount of the rhodopseudomonas palustris, the clostridium butyricum, the oak leaf extract and the aloe leaf extract in each gram of feed is 105-107 cfu, 105-107 cfu, 1-2 mg and 4-8 mg; the preparation method of the oak leaf extract and the aloe leaf extract comprises the following steps: respectively cleaning oak leaves and aloe leaves with distilled water, and ventilating and drying in the shade at 25 DEG C; the preparation method comprises the following steps: respectively homogenizing and crushing oak leaves and aloe leaves by using a homogenizer, and adding an ethanol solution with the volume fraction of 65-85% according to the material-liquid ratio of 0.05-0.1 g/mL; extracting for 1-2.5 hours at normal temperature under the action of ultrasonic waves with the working frequency of 40 KHZ and the power of 80 W; and filling the extract into an ultrafiltration membrane device, filtering to remove impurities under the pressure condition of 0.06 Mpa, and carrying out vacuum concentration and freeze drying to obtain freeze-dried powder.

Description

technical field [0001] The invention belongs to the fields of microbial engineering technology and echinoderm disease prevention and control, and in particular relates to a compound microecological preparation for cultivating sea cucumbers for inhibiting pathogenic Vibrio. Background technique [0002] sea ​​cucumber ( Apostichopus japonicus ) belongs to Echinodermata and Sea Cucumber, and is rich in protein, minerals, vitamins and other natural active substances, and has extremely high nourishing and medicinal value. At present, Apostichopus aquaculture has developed rapidly. "Rotten skin syndrome" is a common disease in the process of sea cucumber cultivation, which has the characteristics of strong fulminance, fast infection speed, and high mortality. Studies have found that pathogenic Vibrio such as Vibrio resplendent, Vibrio harveii, and Vibrio parahaemolyticus are the main pathogenic bacteria that cause "rot skin syndrome" in sea cucumbers. Due to problems of food sa...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/30A23K50/80C12N1/20C12R1/01C12R1/145
CPCA23K10/18A23K10/30A23K50/80C12N1/20
Inventor 王旭达蒋经伟李石磊周遵春叶博关晓燕董颖赵泽龙米锐王摆高杉王晓燕
Owner LIAONING OCEAN & FISHERIES SCI RES INST
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