Biodegradable injection filler microsphere as well as preparation method and application thereof
A filler and microsphere technology, applied in the field of medical cosmetic materials, can solve problems such as unfavorable human health and its industrial application, incomplete removal of residual solvent in freeze-drying process, uneven particle density of PLLA microspheres, etc. Side effects such as nodules, reduction of microbial and bacterial endotoxin levels, and improved quality uniformity
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Embodiment 1
[0081] Example 1 Preparation of PLLA microspheres
[0082] (1) Water phase preparation: add 62.5g of methylcellulose into 25L of purified water, stir at 200rpm, and dissolve;
[0083] (2) Organic phase preparation: add 250g of poly-L-lactic acid into 2.5L of dichloromethane organic solvent, and stir until completely dissolved;
[0084] (3) Emulsification and granulation: mix the organic phase and the aqueous phase with a volume ratio of 1:20 evenly, homogenize at 10000 rpm for 3 minutes, and stir overnight;
[0085] (4) Refining treatment: collect the microspheres by filtration, add 5L of absolute ethanol solution to wash, filter, rinse the filter cake with absolute ethanol, sieve through 200 mesh and 400 mesh sieves, dry at 50°C for 24 hours, and obtain the particle size Microspheres of 20-60 μm.
Embodiment 2
[0086] Example 2 Preparation of PLLA microspheres
[0087] (1) Water phase preparation: add 62.5g of methylcellulose into 25L of purified water, stir at 500rpm, and dissolve;
[0088] (2) Organic phase preparation: add 250g of poly-L-lactic acid into 2.5L of dichloromethane organic solvent, and stir until completely dissolved;
[0089] (3) Emulsification and granulation: mix the organic phase and the aqueous phase with a volume ratio of 1:10 evenly, homogenize at 12000 rpm for 6 minutes, and stir overnight;
[0090] (4) Refining treatment: collect the microspheres by filtration, add 5L of absolute ethanol solution to wash, filter, rinse the filter cake with absolute ethanol, sieve through 200 mesh and 400 mesh sieves, dry at 50°C for 24 hours, and obtain the particle size Microspheres of 20-60 μm.
Embodiment 3
[0091] Example 3 Preparation of PLLA Microspheres
[0092] (1) Water phase preparation: add 62.5g of methylcellulose into 25L of purified water, stir at 300rpm, and dissolve;
[0093] (2) Organic phase preparation: add 250g of poly-L-lactic acid into 2.5L of dichloromethane organic solvent, and stir until completely dissolved;
[0094] (3) Emulsification and granulation: mix the organic phase and the aqueous phase with a volume ratio of 1:6 evenly, homogenize at 15000 rpm for 3 minutes, and stir overnight;
[0095] (4) Refining treatment: collect the microspheres by filtration, add 5L of absolute ethanol solution to wash, filter, rinse the filter cake with absolute ethanol, sieve through 200 mesh and 400 mesh sieves, dry at 50°C for 24 hours, and obtain the particle size Microspheres of 20-60 μm.
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