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Protein related to nitrogen absorption and transformation of rice as well as coding gene and application of protein
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A protein and nitrogen absorption technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of high yield and resource efficiency, and there is no effective solution to improve crop yield potential, improve nitrogen fertilizer utilization efficiency, and increase yield Effect
Active Publication Date: 2022-04-05
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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[0005] At present, researchers have carried out related exploration and research on crop breeding, cultivation management measures and functional gene mining, and have made some important progress, but for the above-mentioned crop production There are no effective solutions to the two major problems of high yield and resource efficiency, high yield and early maturity
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[0142] This example provides a protein derived from Nipponbare rice, which has the functions of improving rice photosynthesis efficiency, promoting nitrogen absorption, promoting early heading and increasing yield. The name of the protein is OsDREB1C, and its sequence is sequence 1 in the sequence listing , in Nipponbare, the coding gene sequence of OsDREB1C is sequence 2, and the genome sequence is 3001-4131 of sequence 3. In sequence 3, positions 1-3000 are the promoter of OsDREB1C gene in Nipponbare genomeDNA.
[0143] Comparing the sequences of rice OsDREB1C with homologous proteins in other plants, it was found that its identities with millet, corn, sorghum, goat grass, wheat, and Brachypodium distachyon were 73.52%, 64.06%, 66.52%, and 66.05%, respectively. %, 66.05%, 65.88% ( figure 1 ).
[0144] 1. Con...
Embodiment 2
[0204] Example 2, Functional detection of OsDREB1C in wheat and Arabidopsis
[0205] 1. Construction of recombinant vector
[0206] Construction of wheat overexpression vector: the full-length CDS of OsDREB1C gene was amplified from rice Nipponbare cDNA by PCR, and the obtained PCR product was combined with pWMB110 vector (Huiyun Liu, KeWang, Zimiao Jia, Qiang Gong, Zhishan Lin, Lipu Du, Xinwu Pei, Xingguo Ye, Efficient induction of haploid plants in wheat by editing of TaMTL using an optimized Agrobacterium-mediated CRISPRsystem, Journal of Experimental Botany, Volume 71, Issue 4, 7 February 7–13 13 Page 2020, https: / / doi.org / 10.1093 / jxb / erz529) were connected to the vector backbone obtained by double digestion with BamHI and SacI, and the obtained recombinant vector with correct sequence was designated as pWMB110-OsDREB1C. pWMB110-OsDREB1C is a recombinant vector obtained by replacing the DNA fragment between the BamHI and SacI recognition sites of the pWMB110 vector with ...
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Abstract
The invention discloses a protein related to nitrogen absorption and transformation of rice as well as a coding gene and application of the protein. The protein related to rice nitrogen absorption and transformation disclosed by the invention is OsDREB1C, the sequence of the protein is a sequence 1 in a sequence table, and the coding gene sequence of the protein is a sequence 2 in the sequence table. Experiments prove that the OsDREB1C and the related biological materials thereof can improve the photosynthetic efficiency of plants, can promote nitrogen absorption and transport, improve the nitrogen content in the plants and grains, can promote heading in advance and can also improve the yield, and the OsDREB1C and the related biological materials thereof have important biological significance and industrial value and are worthy of popularization and application. Good application prospects are realized.
Description
technical field [0001] The invention relates to a protein related to rice nitrogen absorption and transformation, its coding gene and application in the field of biotechnology. Background technique [0002] In agricultural production, applying a large amount of nitrogen fertilizer has always been one of the important measures for high crop yield. The continuous large amount of nitrogen fertilizer input not only increases the cost of planting, but also leads to increasingly serious environmental pollution problems. How to reduce nitrogen fertilizer input in agricultural production and continuously increase crop yield has become a major problem to be solved urgently for the sustainable development of agriculture in my country. Exploring the regulation mechanism and technical approach for the substantial increase of crop yield and efficient utilization of resources is an urgent task for the sustainable development of agriculture in my country. [0003] Heading date is one of ...
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