Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of functionalized nano-selenium in preparation of anti-wound infection drugs

A nano-selenium, functionalized technology, used in antibacterial drugs, drug combinations, pharmaceutical formulations, etc., can solve problems such as unfavorable wound healing, achieve good immune activation ability, accelerate wound repair, and activate immune response.

Pending Publication Date: 2022-04-08
JINAN UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, prolonged release of higher levels of pro-inflammatory cytokines by immune cells is detrimental to wound healing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of functionalized nano-selenium in preparation of anti-wound infection drugs
  • Application of functionalized nano-selenium in preparation of anti-wound infection drugs
  • Application of functionalized nano-selenium in preparation of anti-wound infection drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Synthesis of functionalized nano-selenium

[0043] (1) Get 100mg of CS (chitosan, McLean), LNT (lentinan, Shaanxi Senfu Biotechnology Co., Ltd.), PVP (polyvinylpyrrolidone, McLean) and dissolve them in 10mL of deionized water respectively, and make A stock solution with a concentration of 10 mg / mL; take 173 mg of Na 2 SeO 3 (sodium selenite, Alfa Aisha) and 176mg of Vc (ascorbic acid, McLean) were dissolved in 10mL of deionized water respectively, and made into a stock solution with a concentration of 100mM;

[0044] (2) Take 2mL CS solution, add 0.5mL Na 2 SeO 3 Solution, after adding deionized water to 8mL, stirred on a magnetic stirrer for 5min to make the polymer and Na 2 SeO 3Mix well to form a mixed solution, then add 2 mL of Vc solution dropwise to the mixed solution until the addition of Vc is complete, and stir overnight at room temperature. Place the reacted solution in a dialysis bag of 100,000 kDa and dialyze for 24 hours to remove unreacted polymer to...

Embodiment 2

[0047] Bacteriostatic experiment of functionalized nano-selenium

[0048] Methicillin-resistant Staphylococcus aureus (ATCC43300) was selected in the experiment, inoculated in LB medium, and cultured overnight on a constant temperature shaker at 37°C. The bacteria were then diluted with LB medium to a concentration of 10 7 CFU mL -1 . Add diluted bacteria (5 μL, 10 7 CFU mL -1 ). Then PBS buffer (0.01mmol / L, pH=7.4, as blank control), chitosan nano-selenium (CS-SeNPs), polyvinylpyrrolidone nano-selenium (PVP-SeNPs), lentinan nano-selenium (LNT-SeNPs ) were added to ep tubes to dilute to 500 μL and incubated overnight at 37° C. on a shaking shaker. Dilute a certain number of times, and illustrate the antibacterial effect through the plate coating experiment.

[0049] The result is as figure 1 As shown in Table 1, for MRSA, LNT-SeNPs exhibited a strong antibacterial effect, the minimum inhibitory concentration (MIC) was 8ug / ml, and the minimum bactericidal concentration ...

Embodiment 3

[0053] Experiment of immune activation effect of functionalized nano-selenium

[0054] (1) Effect of functionalized nano-selenium on the maturation of dendritic cells

[0055] DC cells have an important biological characteristic, that is, the maturation of DC cells. DC cells are first stimulated by pathogens or other antigens, then ingest and process the antigens, and gradually differentiate into mature cells. Next, DC cells transfer from peripheral tissues to lymph nodes, spleen, etc., and present antigens to T cells, thereby initiating an immune response and having a bactericidal effect.

[0056] In this experiment, female C57BL / 6 mice were used. After they were sacrificed, the bone marrow of the mouse femur and tibia was aseptically collected, and the red blood cells were lysed to obtain a single cell suspension; after that, they were placed in a culture medium containing GM-CSF cytokines Bone marrow-derived dendritic cells (BMDC) can be obtained by continuing to culture ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses an application of functionalized nano-selenium in preparation of an anti-wound infection drug. The functionalized nano-selenium is one or more of chitosan nano-selenium, polyvinylpyrrolidone nano-selenium or lentinan nano-selenium and other polymer modified nano-selenium. Different charge modifier nano-selenium is synthesized, so that a direct killing effect on pathogenic bacteria can be realized, and pathogenic bacteria infection can be effectively eliminated by immune cells by regulating and controlling an organism immune system. Functional nano-selenium has a positive and effective regulation effect on immune cells of a wound part, is good in immune activation capability, promotes maturation and differentiation of DC cells and NK cells, promotes phagocytosis of macrophages on bacteria, activates immune response and rapidly sterilizes, so that wound repair is accelerated.

Description

technical field [0001] The invention relates to the application of functionalized nano-selenium in the preparation of anti-wound infection drugs. Background technique [0002] A large number of studies have shown that the main pathogenic bacteria of wound infection include Gram-negative bacteria, such as Escherichia coli, Pseudomonas aeruginosa, etc. All developed resistance to broad-spectrum antibiotics. Multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA) infection has become a global treatment problem, and the index commonly used to evaluate the antibacterial activity of antibacterial drugs in clinic is the minimum inhibitory concentration. [0003] At present, the prevention and treatment measures for wound infection of drug-resistant bacteria mainly include the combination of antibacterial drugs and the research and development of new antibacterial drugs (such as nano-silver and other antibacterial agents). However, these strategies have their limita...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K31/722A61K31/77A61K31/79A61K31/785A61K33/04A61P31/04A61P37/02A61P17/02
Inventor 陈填烽许利耕卜庆月
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com