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Protein mulberry leavening agent as well as preparation method and application thereof

A technology for protein mulberry and starter, which is applied to the field of protein mulberry starter and its preparation, can solve the problems of difficulty in unifying cultivation temperature and cultivation time, difficult to achieve precise control, and difficult to control the proportion of mixed bacteria, so as to shorten the fermentation time, Avoid nutrient competition and antagonism, the effect of simple fermentation method

Pending Publication Date: 2022-04-12
SHANGHAI NONGLE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although a variety of probiotic microbial compound starters have obvious advantages over lactic acid bacteria starters, they are prone to pollution and difficult to control the proportion of mixed bacteria due to the mixed culture of multiple bacteria.
For example, the culture temperature of bacteria is generally 28-37°C, the culture temperature of yeast is 25-30°C, and the culture temperature of mold is 25-28°C. It is difficult to unify the culture temperature and culture time of different bacteria.
It is difficult to accurately control the microbial metabolic process, such as the compound bacteria mentioned in CN109156643 A, a method for preparing protein mulberry microbial fermentation feed, including Bifidobacterium, lactic acid bacteria, Saccharomyces cerevisiae, cellulase and Aspergillus oryzae

Method used

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  • Protein mulberry leavening agent as well as preparation method and application thereof
  • Protein mulberry leavening agent as well as preparation method and application thereof
  • Protein mulberry leavening agent as well as preparation method and application thereof

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preparation example Construction

[0022] The invention provides a kind of preparation method of albumen mulberry starter, described preparation method comprises the following steps:

[0023] 1) mixing a mixed solution comprising a carbon source, a nitrogen source, and an inorganic salt with mulberry leaf powder to obtain mixed mulberry leaf powder;

[0024] 2) Inoculate the Bacillus subtilis hlj518 with the preservation number CGMCC No. 13026 into the mixed mulberry leaf powder for fermentation, and obtain the protein mulberry starter after the fermentation is completed.

[0025] In one embodiment, the pH of the mixed solution is 7-9.

[0026] In one embodiment, the carbon source is selected from any one or more of glucose, sucrose or lactose. The carbon source concentration in the mixed solution is 0-10%. The concentration of the carbon source is, for example, 0-2%, 2-4%, 4-6%, 6-8% or 8-10%. Preferably, the concentration of the carbon source is 5-7%. When the concentration of the carbon source is 0, a ce...

Embodiment 1

[0050] A method for improving protein content in mulberry leaves, comprising the following steps:

[0051] 1. Based on the quality of water, add 7% sucrose, 0.5% ammonium sulfate, 0.5% sodium citrate, 0.1% dipotassium hydrogen phosphate, 0.1% potassium dihydrogen phosphate, and 0.05% magnesium sulfate to the water to obtain a mixture solution. Wash fresh mulberry leaves, dry and pulverize them through an 80-mesh sieve to obtain mulberry leaf powder; take mulberry leaf powder as the substrate, according to the mass-volume ratio of mulberry leaf powder:mixed solution=1:0.8 (w / v, the unit is g and ml) were mixed to obtain mixed mulberry leaf powder, sterilized at 121°C for 20 minutes, and cooled for later use;

[0052] 2. Inoculate the activated Bacillus subtilis hlj518 strain into NA liquid medium (formula: 0.5%-1% beef extract, 1-2% yeast powder, 0.5-1% sodium chloride), shake at 28°C at 200rpm Cultivate the bottle for 18 hours to obtain the seed liquid, and inoculate the see...

Embodiment 2

[0056] A method for improving protein content in mulberry leaves, comprising the following steps:

[0057] 1. Based on the quality of water, add 8% sucrose, 2% ammonium sulfate, 1% sodium citrate, 0.05% dipotassium hydrogen phosphate, 0.05% potassium dihydrogen phosphate, and 0.1% magnesium sulfate to water to obtain a mixed solution. Wash fresh mulberry leaves, dry and crush them through an 80-mesh sieve to obtain mulberry leaf powder; use mulberry leaf powder as the substrate, mix according to the mass-volume ratio (w / v) of mulberry leaf powder: mixed solution = 1:1, and mix well Finally, the mixed mulberry leaf powder was obtained, sterilized at 121° C. for 20 minutes, and cooled for later use;

[0058] 2. Inoculate the activated Bacillus subtilis hlj518 strain into NA liquid medium, and culture it in a shaker flask at 28°C and 200rpm for 18 hours to obtain a seed liquid, inoculate the seed liquid into the mixed mulberry leaf powder in step 1, and the inoculation amount is ...

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Abstract

The invention relates to the field of functional feed, in particular to a protein mulberry leavening agent and a preparation method and application thereof.The preparation method of the protein mulberry leavening agent comprises the following steps that 1, a mixed solution containing a carbon source, a nitrogen source and inorganic salt is mixed with mulberry leaf powder, and mixed mulberry leaf powder is obtained; and (2) inoculating bacillus subtilis hlj518 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.13026 into the mixed mulberry leaf powder, and fermenting, so as to obtain the protein mulberry leavening agent after the fermentation is finished. The protein mulberry leavening agent can be used for preparing feed. A single strain is adopted for fermentation, the fermentation mode is simple and convenient, nutrient competition and antagonism possibly existing between compound fermentation strains are avoided, the effect of feed generated through fermentation is more stable, the protein content of the prepared protein mulberry leavening agent can reach 25.75%, and the protein content of mulberry leaves is increased by 71.7%.

Description

technical field [0001] The invention relates to the field of functional feed, in particular to a protein mulberry starter and its preparation method and application. Background technique [0002] Mulberry leaves are the leaves of Moraceae plants. Mulberry trees are widely planted in my country, with strong vitality and rich resources. Mulberry trees are distributed all over the country. At present, the mulberry gardens used for production in my country are mainly concentrated in Guangxi, Sichuan, Jiangsu, Zhejiang, Chongqing, Yunnan and other southern regions. Due to the characteristics of mulberry trees that are resistant to barrenness and drought, there are still a large number of ecological mulberry trees distributed in northern my country. According to incomplete statistics, there are more than 530,000 hectares of ecological mulberry trees in the country. The scale of ecological mulberry planting is expected to further expand with the implementation of heavy metal pollute...

Claims

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Application Information

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IPC IPC(8): A23K10/30A23K10/12A23K20/163A23K20/10A23K50/15A23K20/26A23K50/10A23K50/30A23K50/75
CPCY02P60/87
Inventor 樊杰郝祥蕊吴小珍丁千岭杨亮亮李慧张红艳冯镇泰
Owner SHANGHAI NONGLE BIOLOGICAL PROD
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