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Oligosaccharide composition extracted from traditional Chinese medicines as well as preparation method and application of oligosaccharide composition

A technology of oligosaccharide and composition, applied in the field of oligosaccharide composition and its preparation, to achieve good effect, inhibit inflammation, and promote the effect of gastric cancer cell apoptosis

Pending Publication Date: 2022-04-12
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no research on the compatibility of Codonopsis pilosula, red ginseng, rehmannia glutinosa and hawthorn oligosaccharides
However, there is no research on the effect of Codonopsis pilosula, red ginseng, rehmannia glutinosa, hawthorn oligosaccharides and their compatible compositions on the treatment of gastric precancerous lesions.

Method used

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  • Oligosaccharide composition extracted from traditional Chinese medicines as well as preparation method and application of oligosaccharide composition
  • Oligosaccharide composition extracted from traditional Chinese medicines as well as preparation method and application of oligosaccharide composition
  • Oligosaccharide composition extracted from traditional Chinese medicines as well as preparation method and application of oligosaccharide composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The impact of each compatible composition oligosaccharide on the general signs of rats:

[0044] 1. Experimental method:

[0045] 120 Wistar male rats were randomly divided into 11 groups, 20 in the model group, the normal group, the positive control group (Vizyme 0.27g / kg), the low-dose treatment group of Codonopsis red ginseng and Hawthorn oligosaccharides (150 Codonopsis oligosaccharides) Sugar, 50 parts of red ginseng oligosaccharides, 75 parts of rehmannia oligosaccharides, 25 parts of hawthorn oligosaccharides combination; 1.3g / kg), Codonopsis red ginseng Rehmannia hawthorn oligosaccharides high-dose treatment group (150 parts of Codonopsis oligosaccharides , 50 parts of red ginseng oligosaccharides, 75 parts of rehmannia oligosaccharides, 25 parts of hawthorn oligosaccharides combination; 5.2g / kg), Codonopsis red ginseng and rehmannia oligosaccharides low-dose treatment group (150 parts of Codonopsis oligosaccharides, 50 parts Red ginseng oligosaccharide, 75 par...

Embodiment 2

[0052] Effects of the oligosaccharides of each compatible composition on the pathological changes of rat gastric mucosa:

[0053] 1. Experimental method:

[0054] On the basis of confirming the successful modeling of gastric precancerous lesions in the model group rats, food and water were fasted the day before the experiment. On the day of the experiment, 7% chloral hydrate (5 mL / kg) was injected intraperitoneally for anesthesia. After blood collection from the abdominal aorta, gastric tissue was dissected. The morphological changes of gastric mucosa color, gastric wall thickness, extensibility, erosion, bleeding points and hyperplasia nodules were observed with naked eyes. Stomach tissue was fixed in 4% paraformaldehyde solution for 24 hours, then dehydrated with high-concentration ethanol and xylene and embedded in paraffin, cut into 4 μm thick sections, stained with HE, and observed the gastric mucosal glands of rats in each group with a light microscope Atrophy, intesti...

Embodiment 3

[0059] Effects of oligosaccharides of each compatible composition on blood routine in rats with gastric precancerous lesions:

[0060] 1. Experimental method:

[0061] The BC-3000 automatic blood cell analyzer was used to detect the counts of white blood cells (WBC), red blood cells (RBC), platelets (PLT), lymphocytes (W-SCC) and hemoglobin (HGB) in rat blood, and calculate the platelet / lymph Cell Ratio (PLR) Ratio.

[0062] 2. Experimental results:

[0063] As shown in Table 1, compared with the normal group, the red blood cell count (RBC) and hemoglobin content (HGB) in the blood of the rats in the model group were significantly reduced (P0.05). Compared with the model group, the high dose of Dangshen, red ginseng and hawthorn oligosaccharides (5.2g / kg), the high dose of dangshen, red ginseng and hawthorn oligosaccharides (2.28g / kg), and the high dose of red ginseng and rehmannia oligosaccharides (0.64g / kg) and Codonopsis oligosaccharide high dose (0.6g / kg) can significa...

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Abstract

The invention provides an oligosaccharide composition extracted from traditional Chinese medicines. The oligosaccharide composition is prepared from the following raw materials in parts by weight: 0 to 200 parts of radix codonopsis oligosaccharide, 1 to 100 parts of radix ginseng rubra oligosaccharide, 50 to 100 parts of radix rehmanniae preparata oligosaccharide and 0 to 50 parts of fructus crataegi oligosaccharide. The invention also provides a preparation method of the compound and application of the compound in preparation of preparation products for treating gastric precancerous lesions. The oligosaccharide composition has the characteristics and advantages that a preparation product prepared from the oligosaccharide composition has a very good effect, is safe and does not have obvious toxic or side effects. Experiments prove that on the animal level, the oligosaccharide composition has the effects of inhibiting inflammation, resisting oxidation and promoting cell apoptosis; at the cellular level, the oligosaccharide composition can inhibit hypoxia-induced normal gastric mucosa GES-1 cell apoptosis and gastric cancer AGS cell proliferation.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extracts, in particular to an oligosaccharide composition extracted from traditional Chinese medicines and its preparation method and application. Background technique [0002] Oligosaccharides are ubiquitous components in traditional Chinese medicine, not only as energy storage components, but also as important information substances in organisms, with biological functions such as information recognition, information transmission, and cell defense. Studies have shown that oligosaccharides have various pharmacological activities such as enhancing immunity, anti-tumor, anti-oxidation, anti-inflammation, regulating intestinal flora, and lowering blood sugar. Because of its important biological activity and huge medicinal potential, it has gradually attracted widespread attention at home and abroad. [0003] Codonopsis ginseng, red ginseng, rehmannia glutinosa and hawthorn all c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/804A61P1/00A61P35/00A61K31/702A61K31/715A61K36/258A61K36/344A61K36/734
Inventor 胡芳弟闫巧韩佳冰王燕萍李成楠李文
Owner LANZHOU UNIVERSITY
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