Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Photo-crosslinking injectable nanofiber-hydrogel compound as well as preparation method and application thereof

A nanofiber and composite technology, which is applied in the field of photocrosslinking injectable nanofiber-hydrogel composites and their preparation, can solve the problems of non-injection, lack of skin repair mechanical properties, etc., and achieve good biocompatibility , the effect of enhancing mechanical properties

Active Publication Date: 2022-04-12
SICHUAN UNIV
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the existing hydrogels used for skin repair, it is difficult to balance the mechanical properties and cell infiltration, and the existing electrospun fiber membranes are beneficial to the growth and infiltration of cells, but they cannot be injected and do not have the mechanical properties required for skin repair. , the present invention provides a photocrosslinkable injectable nanofiber-hydrogel composite and its preparation method, as well as the application of the composite in the field of skin repair, so as to improve the material without reducing the porosity and specific surface area of ​​the material The mechanical and tissue adhesion properties of the material improve the skin repair performance of the material

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Photo-crosslinking injectable nanofiber-hydrogel compound as well as preparation method and application thereof
  • Photo-crosslinking injectable nanofiber-hydrogel compound as well as preparation method and application thereof
  • Photo-crosslinking injectable nanofiber-hydrogel compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] In this embodiment, the preparation of hyaluronic acid-benzyl ester (HA-Bn) nanofibers, the steps are as follows:

[0050] (1) Dissolve sodium hyaluronate with a molecular weight of 1000kDa in deionized water to form a sodium hyaluronate solution with a concentration of 10 mg / mL, add Dowex resin to the solution and react for 4 hours until the pH value is stable, and remove the Dowex resin by centrifugation. Then, tetrabutylammonium hydroxide was added to the obtained liquid phase until the pH value was stable, and then the obtained reaction solution was freeze-dried to obtain tetrabutylammonium hyaluronic acid, which was designated as HA-TBA.

[0051] In this step, the addition amount of Dowex resin is 3 times of the mass of sodium hyaluronate, and the addition amount of tetrabutylammonium hydroxide is 2.2 times of the mass of sodium hyaluronate.

[0052] (2) Dissolve HA-TBA in anhydrous dimethyl sulfoxide (DMSO) to form a HA-TBA solution with a concentration of 10mg / mL...

Embodiment 2

[0058] In this example, methacrylylated gelatin (GelMA), methacrylated hyaluronic acid (HAMA), and methacrylated dopamine (Dopa-MA) were prepared respectively, and the steps were as follows:

[0059] (1) Weigh 100g of gelatin and dissolve it in 1.5L of sodium carbonate-sodium bicarbonate buffer solution, add 40mL of methacrylic anhydride dropwise to the resulting solution, react at 50°C for 4h, dialyze the resulting reaction solution and freeze-dry to obtain GelMA .

[0060] (2) Weigh 25g of sodium hyaluronate with a molecular weight of 1000kDa and dissolve it in 1L of deionized water, place the resulting solution on an ice bath, adjust the pH value of the solution to greater than 8.5 with 1mol / L NaOH solution, and slowly add 56.25mL of methacrylic anhydride, during the dropwise addition process, use 1mol / L NaOH solution to maintain the pH value of the reaction system greater than 8.5, remove the ice bath after 4 hours of reaction, continue the reaction for 12 hours, dialyze t...

Embodiment 3

[0070] In this example, a photocrosslinkable injectable nanofiber-hydrogel composite was prepared.

[0071] (1) Dissolve GelMA, HAMA, Dopa-MA and photoinitiator LAP in deionized water to obtain a raw material mixture under dark conditions, and then fully disperse the HA-Bn nanofibers in the raw materials under the action of ultrasound under dark conditions In the mixed solution, the gel precursor solution III was obtained, which was irradiated with 405nm excitation light for 1 min to trigger the cross-linking reaction of GelMA, HAMA and Dopa-MA to obtain the nanofiber-hydrogel composite, which was designated as composite III.

[0072] The mass ratio of GelMA, HAMA, Dopa-MA, LAP to HA-Bn nanofibers was 10:1:0.5:0.5:1, and the concentration of GelMA in gel precursor solution III was 0.01 g / mL.

[0073] (2) Dissolve GelMA, HAMA, Dopa-MA and photoinitiator LAP in deionized water to obtain a raw material mixture under dark conditions, and then fully disperse the HA-Bn nanofibers in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
lengthaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a photo-crosslinking injectable nanofiber-hydrogel compound and a preparation method thereof. The compound is prepared from composite hydrogel and hydrophobic modified hyaluronic acid nanofibers which are uniformly dispersed in the composite hydrogel, the composite hydrogel is formed by polymerizing gelatin which is modified by carbon-carbon double bonds and can be photopolymerized, hyaluronic acid which is modified by carbon-carbon double bonds and can be photopolymerized, and dopamine which is modified by carbon-carbon double bonds and can be photopolymerized in the presence of a photoinitiator under the illumination condition. On the basis that the porosity and the specific surface area of the material are not reduced, the mechanical property and the tissue adhesion performance of the material are improved, and the skin repair performance of the material is improved. The invention also provides an application of the photo-crosslinking injectable nanofiber-hydrogel compound in the field of preparation of skin repair materials.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and relates to a photocrosslinkable injectable nanofiber-hydrogel composite and a preparation method and application thereof. Background technique [0002] Common skin repair strategies mainly include functional dressing therapy, growth factor / stem cell therapy, and artificial skin. Functional dressings can provide a moist environment conducive to wound repair, and may also have biological properties such as antibacterial, hemostasis, and anti-inflammation. However, the clinical evidence for accelerating wound healing is limited, and the current functional dressings can only be used as a local healing environment. Auxiliary means. The wound healing process is regulated by a variety of growth factors, simulating the microenvironment of tissue repair under physiological conditions, and locally releasing exogenous growth factors to promote wound regeneration is an effective means of re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08F289/00C08F251/00C08F220/58C08F2/48D01F9/00A61L15/26A61L15/28A61L15/32A61L15/42A61L15/44A61L27/48A61L27/50A61L27/52A61L27/54A61L27/60
Inventor 孙勇樊渝江韩笑雯
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com