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Klebsiella capable of automatically generating biosurfactant and degrading benzene series and application of Klebsiella

A Klebsiella, biological surface technology, applied in the field of environmental restoration and microorganisms, to achieve good emulsification effect

Active Publication Date: 2022-04-12
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there have been no relevant reports or reports that Klebsiella can degrade gaseous p-xylene and at the same time produce biosurfactants to improve the degradation efficiency of gaseous hydrophobic p-xylene

Method used

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  • Klebsiella capable of automatically generating biosurfactant and degrading benzene series and application of Klebsiella
  • Klebsiella capable of automatically generating biosurfactant and degrading benzene series and application of Klebsiella
  • Klebsiella capable of automatically generating biosurfactant and degrading benzene series and application of Klebsiella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The isolation and screening of embodiment 1 Klebsiella HN02

[0033] Inorganic salt culture solution used: every 1000mL inorganic salt culture solution contains 1.2g K 2 HPO 4 ·3H 2 O, 1.2g KH 2 PO 4 , 0.2g MgSO 4 ·7H 2 O, 0.4g NH 4 Cl, 0.01g FeSO 4 ·7H 2 O and 1mL trace element aqueous solution. Among them, every 1000mL trace element aqueous solution contains 0.2g CaCl 2 , 0.2g MnSO4 4H 2 O, 0.1g CuSO 4 ·5H 2 O, 0.2g ZnSO 4 ·7H 2 O, 0.09g CoCl 2 ·6H 2 O, 0.12g Na 2 MoO 4 2H 2 O and 0.006g H 3 BO 3 .

[0034] The Klebsiella HN02 in this example was isolated and screened from the activated sludge in the aerobic pool of a papermaking wastewater treatment plant in Hainan Province. Take the sludge discharged from the aerobic tank of the papermaking sewage treatment plant, put it in a 500mL bottle, add 2 times the volume of papermaking black liquor to the sludge, adjust the pH to 8.3, seal the bottle, and culture it on a shaking table at a temperature ...

Embodiment 2

[0055] This example is the application of Klebsiella HN02 in environmental restoration, which can degrade p-xylene in the environment. The environment includes the atmosphere, water body or soil.

[0056] The p-xylene degradation ability of Klebsiella (Klebsiella) HN02 obtained by screening of the present invention is tested:

[0057] According to the needs of the degradation experiment, configure the inorganic salt medium: add 100 mL of inorganic salt solution to a 500 mL headspace bottle (every 1000 mL of inorganic salt culture solution contains: 1.2 g K 2 HPO 4 ·3H 2 O, 1.2g KH 2 PO 4 , 0.2g MgSO 4 ·7H 2 O, 0.4g NH 4 Cl, 0.01g FeSO 4 ·7H 2 O and 1mL trace element aqueous solution. Among them, every 1000mL trace element aqueous solution contains: 0.2g CaCl 2 , 0.2g MnSO 4 4H 2 O, 0.1g CuSO 4 ·5H 2 O, 0.2g ZnSO 4 ·7H 2 O, 0.09g CoCl 2 ·6H 2 O, 0.12g Na 2 MoO 4 2H 2 O and 0.006g H 3 BO 3 ). Autoclave at 121°C for 15 minutes. First, the screened Klebsi...

Embodiment 3

[0063] This example is a method for demonstrating the ability of Klebsiella HN02 to produce biosurfactants.

[0064] The biosurfactant ability produced by Klebsiella (Klebsiella) HN02 screened by the present invention is tested:

[0065] Klebsiella was cultured under the condition that the substrate concentration was 21 mg / L, and the culture conditions were referred to in Example 2. After p-xylene was completely degraded, the supernatant of the bacteria liquid was taken to measure the emulsifying ability. The specific method is to add 3mL peanut oil to the test tube, then add the same amount of cell-free supernatant obtained after centrifuging the bacterial liquid into the same test tube, shake the test tube vigorously for 2 minutes, then let it stand for 24 hours, and observe the height of the emulsified layer after 24 hours . Emulsification index = (height of emulsified layer / total height of liquid in test tube) × 100%.

[0066] After measuring, the emulsification index o...

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Abstract

The invention discloses Klebsiella sp. Capable of automatically generating a biosurfactant and degrading benzene series and application of the Klebsiella sp. The name of the strain is Klebsiella sp.HN02, the strain is preserved in the strain preservation center (GDMCC) of the Guangdong Institute of Microbiology, the preservation number is GDMCC No: 62011, and the preservation date is November 4, 2021. The strain is a Klebsiella bacterium, is gram-negative, is in a short rod shape, and is partially approximately spherical. And after being cultured on an NA plate for 2 days, the bacterial colony is round, convex, smooth in surface and tidy in edge. The Klebsiella HN02 disclosed by the invention can be used for environmental remediation, the strain can take p-xylene as a carbon source to be derived from a glycolipid-producing biosurfactant, the gas-liquid two-phase mass transfer rate in a system is improved, the environmental pollutant p-xylene is degraded, the degradation efficiency is very high, 100% degradation of 21 mg / L p-xylene is realized within 72 hours, and 100% degradation of 26 mg / L p-xylene is realized within 120 hours.

Description

technical field [0001] The invention relates to the technical field of microbes and the technical field of environmental restoration, in particular to a Klebsiella strain capable of producing biosurfactants and degradable benzene series and its application. Background technique [0002] Paraxylene (PX) is a typical volatile organic compound (VOC). It is an isomer of xylene. Mainly used in the production of terephthalic acid (TPA) and as the main precursor for the production of polyethylene terephthalate, widely used in the production of plastics, films and synthetic fibers. Taking China as an example, even if a large amount of p-xylene is produced every year, it still needs to import tens of millions of tons of p-xylene to meet the needs of industrial production. Paraxylene evaporates easily, so it often enters the air, and rarely exists in high concentrations in surface water or topsoil, which also contributes to air pollution problems to a certain extent. P-xylene is po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34B09C1/10B01D53/72B01D53/84C12R1/22C02F101/32C02F103/28
CPCY02A50/20
Inventor 孙蕾王岩万顺刚袁丹王旭耿伟政苏沁华
Owner HAINAN UNIVERSITY