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New application of polypeptide, antibacterial gel material and local drug delivery system

A gel material and delivery system technology, applied in the field of biomedicine, can solve problems such as hypersensitivity, repeated infection, and bacterial drug resistance, and achieve the effects of less raw materials, faster wound infection, and good biological safety

Pending Publication Date: 2022-04-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, few antibiotics are available for topical use, mainly due to hypersensitivity reactions, superinfections, bacterial resistance, etc.

Method used

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  • New application of polypeptide, antibacterial gel material and local drug delivery system
  • New application of polypeptide, antibacterial gel material and local drug delivery system
  • New application of polypeptide, antibacterial gel material and local drug delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Preparation and Characterization of Example Oxidized Dextran (ODEX)

[0097] Take 0.8552g of sodium periodate and dissolve in 8ml of ultrapure water, and dissolve 1g of dextran in 10ml of ultrapure water. Slowly add sodium periodate dropwise into the dextran solution with stirring. Then the mixture was stirred at room temperature in the dark for 4 h. After the 4h reaction, 5ml of ethylene glycol was added and stirred for 5 minutes to neutralize excess sodium periodate. Afterwards, the reaction solution was put into a dialysis bag (molecular weight cut-off 14kDa) and dialyzed in ultrapure water for 48h. After dialysis, the oxidized dextran was obtained by freeze-drying. The structural characterization of oxidized dextran was obtained by Fourier transform infrared spectroscopy (FTIR). The degree of oxidation was obtained by colorimetric titration with hydroxylamine hydrochloride.

[0098] According to the FTIR results, it can be seen at 1731cm -1 Compared with befor...

Embodiment 2

[0105] Embodiment 2, preparation and characterization of hydrogel

[0106] 1. Preparation of DP7-ODEX hydrogel

[0107] Weigh an appropriate amount of antimicrobial peptide DP7 (VQWRIRVAVIRK) powder and dissolve it in PBS to form a solution of 6% (wt%, 100 μl of water (multiplied by a density of about 100 μg) containing 6 μg DP7), then take an appropriate amount of oxidized dextran and dissolve it in PBS to form 10 % (wt%) solution. Mix equal volumes of DP7 solution and oxidized dextran solution at room temperature and let stand for 5 minutes to obtain DP7-ODEX hydrogel (final concentration DP7 3%, ODEX 5%) after cross-linking and curing.

[0108] 2. Preparation of hydrogel (CAZ-DP7-ODEX) loaded with ceftazidime (CAZ)

[0109] First use PBS to dissolve an appropriate amount of CAZ, then add DP7 to the CAZ solution to prepare a solution containing ceftazime, 6% (wt%) DP7, and then equivolume the DP7 solution containing ceftazime with the ODEX solution (10%, wt%) Mix and stan...

Embodiment 3

[0117] Embodiment three, the dissolution rate measurement of DP7-ODEX hydrogel

[0118] The erosion rate of the hydrogel was measured in two media of pH 5.0 and pH 7.4, because DP7 and ODEX were cross-linked by Schiff base, so DP7-ODEX hydrogel may have pH-sensitive properties. may accelerate degradation.

[0119] 200μl DP7-ODEX gel (DP7 3%, ODEX 5%, wt%) was prepared at the bottom of 1.5ml EP tubes, and then 1ml of buffered saline solution with pH 5.0 or pH 7.4 was added and shaken at 37°C Incubated in bed (220rpm / min), removed the release medium at the specified time point, and measured the weight together with EP, so as to calculate the curve of the mass of the remaining hydrogel as a function of time ( Figure 7 ). The weight of the hydrogel in the two media did not change significantly in the first 12 hours, and the hydrogel in the pH 5.0 medium began to degrade after 12 hours, and only about 30% of the weight remained at 72 hours.

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Abstract

The invention belongs to the field of biological medicine, and particularly relates to novel application of antibacterial peptide and hydrogel prepared from the antibacterial peptide and oxidized polysaccharide. The invention aims to solve the technical problems that at present, external antibiotics are easy to cause drug resistance of pathogenic bacteria and common antibiotics are poor in treatment effect on multidrug-resistant bacteria. According to the technical scheme for solving the technical problem, the gel material prepared from the antibacterial peptide and the oxidized polysaccharide is provided, and meanwhile, antibiotics or other biological treatment active ingredients can be loaded. The hydrogel provided by the invention has good capability of inhibiting common pathogenic bacteria or multidrug resistance bacteria, and can promote migration of fibroblasts at the wound; after the antibiotics are added, a good combined effect can be achieved, the dosage of the antibiotics can be greatly reduced when multidrug-resistant bacteria are treated, meanwhile, the drug-resistant process of the bacteria is delayed, and good clinical application prospects are achieved.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a new application of polypeptides, an antibacterial hydrogel material prepared from antibacterial peptides and polysaccharides, and a local drug delivery system. Background technique [0002] Acute skin trauma usually heals within 8 to 12 weeks, but healing and treatment times can be prolonged if the wound site becomes infected, especially with multidrug-resistant (MDR) bacteria for which there are no effective treatments . Because multidrug-resistant bacteria can form biofilms composed of extracellular polymers on the surface of wounds, they are resistant to antibiotics, and are difficult to be eliminated by conventional means, and are prone to repeated infections. In theory, the most ideal way to treat wound infection is to give antibiotics locally, because this can increase the local drug concentration and avoid systemic drug allergic reactions and other toxic side effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K9/06A61K38/10A61K45/06A61K47/61A61P17/02A61P31/04
Inventor 杨莉
Owner SICHUAN UNIV
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