High-flux liquid-phase suspension type biochip for virus detection and preparation method of high-flux liquid-phase suspension type biochip
A biochip and virus detection technology, applied in the biological field, can solve the problems of low test sensitivity and poor dispersion of quantum dots, and achieve the effects of good mechanical flexibility, high sensitivity and large specific surface area
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[0024] Example 1
[0025] The 30ml concentration of 0.01g / ml graphene quantum dot solution and 30ml of graphene nanosheet dispersion with a concentration of 0.01g / ml were mixed with 500W under sonication for 100min to obtain a composite dispersion solution; after mixing 4g of microcrystalline cellulose with 100ml of sulfuric acid solution with a concentration of 65wt%, it was warmed up at a rate of 1 °C / min to 40 °C for 1h, and after the end of the reaction, 1000ml of deionized water was added to terminate the reaction, and the resulting reaction liquid was centrifuged. The dispersion liquid prepared after centrifugation was re-dispersed by centrifugation was freeze-dried at -20 °C for 20h to obtain nanocellulose crystals; the nanocellulose crystals prepared by 1g were dispersed in 100ml deionized water, and then 0.07g TEMPO, 0.17g NaBr were added, and after stirring and dissolving, 40ml of sodium hypochlorite solution with a concentration of 10wt% was added, and 1mol / L sodium hydro...
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[0027] Example 2
[0028] The 30 ml of graphene quantum dot solution with a concentration of 0.05 g / ml and the graphene nanosheet dispersion of 0.05 g / ml of 30 ml were mixed with 1000 W under sonication for 100 min to obtain a composite dispersion solution; after mixing 5 g of microcrystalline cellulose with a sulfuric acid solution of 100 ml of 65 wt%, it was warmed up at a rate of 1 °C / min to 40 °C for 2 h, and after the end of the reaction, 1000 ml of deionized water was added to terminate the reaction, and the resulting reaction liquid was centrifuged The dispersion liquid prepared after centrifugation of the precipitate was freeze-dried at -20 °C for 20 h to obtain nanocellulose crystals; the nanocellulose crystals prepared by 1g were dispersed in 100ml of deionized water, and then 0.07g TEMPO, 0.18g NaBr were added, 50ml of sodium hypochlorite solution with a concentration of 10wt% was added after stirring and dissolving, and 1mol / L sodium hydroxide solution was added to adj...
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[0030] Example 3
[0031] The 30 ml of graphene quantum dot solution with a concentration of 0.02 g / ml and the graphene nanosheet dispersion of 0.02 g / ml of 30 ml were mixed with 800 W for 100 min to obtain a composite dispersion; after mixing 4.5 g of microcrystalline cellulose with a sulfuric acid solution of 100 ml of 65 wt%, it was warmed up at a rate of 1 °C / min to 40 °C for 2 h, and after the end of the reaction, 1000 ml of deionized water was added to terminate the reaction. The resulting reaction liquid was centrifuged, and the dispersion prepared after centrifugation of the precipitate was freeze-dried at ~-20 °C for 20 h to obtain nanocellulose crystals; the nanocellulose crystals prepared of 1g were dispersed in 100ml deionized water, and then 0.07g TEMPO, 0.18g NaBr were added, 50ml of sodium hypochlorite solution with a concentration of 10wt% was added after stirring and dissolving, and 1mol / L sodium hydroxide solution was added to adjust the pH to 11, After the react...
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