High-flux liquid-phase suspension type biochip for virus detection and preparation method of high-flux liquid-phase suspension type biochip

A biochip and virus detection technology, applied in the biological field, can solve the problems of low test sensitivity and poor dispersion of quantum dots, and achieve the effects of good mechanical flexibility, high sensitivity and large specific surface area

Pending Publication Date: 2022-04-26
苏州育德扬生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this technology, the problem of poor dispersion of qua

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024] Example 1

[0025] The 30ml concentration of 0.01g / ml graphene quantum dot solution and 30ml of graphene nanosheet dispersion with a concentration of 0.01g / ml were mixed with 500W under sonication for 100min to obtain a composite dispersion solution; after mixing 4g of microcrystalline cellulose with 100ml of sulfuric acid solution with a concentration of 65wt%, it was warmed up at a rate of 1 °C / min to 40 °C for 1h, and after the end of the reaction, 1000ml of deionized water was added to terminate the reaction, and the resulting reaction liquid was centrifuged. The dispersion liquid prepared after centrifugation was re-dispersed by centrifugation was freeze-dried at -20 °C for 20h to obtain nanocellulose crystals; the nanocellulose crystals prepared by 1g were dispersed in 100ml deionized water, and then 0.07g TEMPO, 0.17g NaBr were added, and after stirring and dissolving, 40ml of sodium hypochlorite solution with a concentration of 10wt% was added, and 1mol / L sodium hydro...

Example Embodiment

[0027] Example 2

[0028] The 30 ml of graphene quantum dot solution with a concentration of 0.05 g / ml and the graphene nanosheet dispersion of 0.05 g / ml of 30 ml were mixed with 1000 W under sonication for 100 min to obtain a composite dispersion solution; after mixing 5 g of microcrystalline cellulose with a sulfuric acid solution of 100 ml of 65 wt%, it was warmed up at a rate of 1 °C / min to 40 °C for 2 h, and after the end of the reaction, 1000 ml of deionized water was added to terminate the reaction, and the resulting reaction liquid was centrifuged The dispersion liquid prepared after centrifugation of the precipitate was freeze-dried at -20 °C for 20 h to obtain nanocellulose crystals; the nanocellulose crystals prepared by 1g were dispersed in 100ml of deionized water, and then 0.07g TEMPO, 0.18g NaBr were added, 50ml of sodium hypochlorite solution with a concentration of 10wt% was added after stirring and dissolving, and 1mol / L sodium hydroxide solution was added to adj...

Example Embodiment

[0030] Example 3

[0031] The 30 ml of graphene quantum dot solution with a concentration of 0.02 g / ml and the graphene nanosheet dispersion of 0.02 g / ml of 30 ml were mixed with 800 W for 100 min to obtain a composite dispersion; after mixing 4.5 g of microcrystalline cellulose with a sulfuric acid solution of 100 ml of 65 wt%, it was warmed up at a rate of 1 °C / min to 40 °C for 2 h, and after the end of the reaction, 1000 ml of deionized water was added to terminate the reaction. The resulting reaction liquid was centrifuged, and the dispersion prepared after centrifugation of the precipitate was freeze-dried at ~-20 °C for 20 h to obtain nanocellulose crystals; the nanocellulose crystals prepared of 1g were dispersed in 100ml deionized water, and then 0.07g TEMPO, 0.18g NaBr were added, 50ml of sodium hypochlorite solution with a concentration of 10wt% was added after stirring and dissolving, and 1mol / L sodium hydroxide solution was added to adjust the pH to 11, After the react...

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Abstract

The invention discloses a high-flux liquid-phase suspension type biological chip for virus detection. The high-flux liquid-phase suspension type biological chip comprises a suspension medium and multifunctional microspheres uniformly dispersed in the suspension medium, the multifunctional microsphere comprises an inorganic/polymer composite microsphere and a biological probe coated on the surface of the inorganic/polymer composite microsphere, the inorganic/polymer microsphere comprises a graphene nanosheet, a graphene quantum dot shell layer dispersed on the surface of the graphene nanosheet and a polymer layer modified on the surface of the graphene quantum dot shell layer; the polymer layer is a carboxylated nano cellulose crystal/polyacrylamide binary network gel layer. The invention also discloses a preparation method of the liquid-phase suspension type biological chip. The liquid-phase suspension type biological chip prepared by the invention is good in monodispersity and high in sensitivity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a multifunctional inorganic / polymer composite microsphere, its preparation method and its application in virus detection. Background technique [0002] In the past decade, semiconductor quantum dots, as a nano-sized crystalline material, have attracted great attention from many researchers in the field of biomedicine. Compared with traditional dyes and fluorescent proteins, quantum dots have unique optical properties, such as emission spectra that vary with size, high brightness, super photostability against photobleaching, and simultaneous excitation of multiple fluorescences. These characteristics make quantum dots and their conjugates a reliable optical marker, which has a wide range of applications in biological fields such as virus detection. [0003] Patent CN201210073965.4 provides a method for homogeneous immunodetection of various viruses based on quantum dots and graphene ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/576G01N33/551G01N33/548G01N33/545G01N33/58C08F251/02C08F220/56C08F222/38G01N21/64
CPCG01N33/56983G01N33/5761G01N33/54313G01N33/588C08B15/04C08F251/02G01N21/6428G01N2469/10C08F220/56C08F222/385
Inventor 李育修
Owner 苏州育德扬生物技术有限公司
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