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High-flux liquid-phase suspension type biochip for virus detection and preparation method of high-flux liquid-phase suspension type biochip

A biochip and virus detection technology, applied in the biological field, can solve the problems of low test sensitivity and poor dispersion of quantum dots, and achieve the effects of good mechanical flexibility, high sensitivity and large specific surface area

Pending Publication Date: 2022-04-26
苏州育德扬生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this technology, the problem of poor dispersion of quantum dots often occurs, resulting in low test sensitivity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 30ml concentration is 0.01g / ml graphene quantum dot solution and 30ml concentration is 0.01g / ml graphene nano sheet dispersion liquid mixing 500W and ultrasonic treatment 100min, makes composite dispersion liquid; Mix 4g microcrystalline cellulose and 100ml After the sulfuric acid solution with a concentration of 65wt% was mixed and stirred evenly, the temperature was raised to 40°C at a rate of 1°C / min and reacted for 1 hour. After the reaction was completed, 1000ml of deionized water was added to terminate the reaction. The obtained reaction solution was centrifuged, and the precipitate obtained by centrifugation was redispersed. The resulting dispersion was freeze-dried at -20°C for 20 hours to obtain nanocellulose crystals; disperse 1 g of the above-prepared nanocellulose crystals in 100 ml of deionized water, then add 0.07 g of TEMPO and 0.17 g of NaBr, and stir After dissolving, add 40ml of sodium hypochlorite solution with a concentration of 10wt%, and add 1mol / L ...

Embodiment 2

[0028] 30ml concentration is 0.05g / ml graphene quantum dot solution and 30ml concentration is 0.05g / ml graphene nano sheet dispersion liquid mixing 1000W and ultrasonic treatment 100min, makes composite dispersion liquid; 5g microcrystalline cellulose and 100ml After the sulfuric acid solution with a concentration of 65wt% was mixed and stirred evenly, the temperature was raised to 40°C at a rate of 1°C / min and reacted for 2 hours. After the reaction was completed, 1000ml of deionized water was added to terminate the reaction. The obtained reaction solution was centrifuged, and the precipitate obtained by centrifugation was redispersed. The resulting dispersion was freeze-dried at -20°C for 20 hours to obtain nanocellulose crystals; disperse 1 g of the above-prepared nanocellulose crystals in 100 ml of deionized water, then add 0.07 g TEMPO and 0.18 g NaBr, and stir After dissolving, add 50ml of sodium hypochlorite solution with a concentration of 10wt%, and add 1mol / L sodium h...

Embodiment 3

[0031] 30ml concentration is the graphene quantum dot solution of 0.02g / ml and 30ml concentration is the graphene nanosheet dispersion liquid of 0.02g / ml mixing 800W and supersonic treatment 100min, makes composite dispersion liquid; 4.5g microcrystalline cellulose and After mixing and stirring 100ml of sulfuric acid solution with a concentration of 65wt%, the temperature was raised to 40°C at a rate of 1°C / min and reacted for 2 hours. After the reaction was completed, 1000ml of deionized water was added to terminate the reaction. The dispersion liquid obtained after dispersion was freeze-dried at ~-20°C for 20 hours to obtain nanocellulose crystals; disperse 1g of the above-prepared nanocellulose crystals in 100ml deionized water, and then add 0.07g TEMPO, 0.18g NaBr After stirring and dissolving, add 50ml of sodium hypochlorite solution with a concentration of 10wt%, and add 1mol / L of sodium hydroxide solution to adjust the pH to 11, add 50ml of ethanol dropwise to terminate ...

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PUM

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Abstract

The invention discloses a high-flux liquid-phase suspension type biological chip for virus detection. The high-flux liquid-phase suspension type biological chip comprises a suspension medium and multifunctional microspheres uniformly dispersed in the suspension medium, the multifunctional microsphere comprises an inorganic / polymer composite microsphere and a biological probe coated on the surface of the inorganic / polymer composite microsphere, the inorganic / polymer microsphere comprises a graphene nanosheet, a graphene quantum dot shell layer dispersed on the surface of the graphene nanosheet and a polymer layer modified on the surface of the graphene quantum dot shell layer; the polymer layer is a carboxylated nano cellulose crystal / polyacrylamide binary network gel layer. The invention also discloses a preparation method of the liquid-phase suspension type biological chip. The liquid-phase suspension type biological chip prepared by the invention is good in monodispersity and high in sensitivity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a multifunctional inorganic / polymer composite microsphere, its preparation method and its application in virus detection. Background technique [0002] In the past decade, semiconductor quantum dots, as a nano-sized crystalline material, have attracted great attention from many researchers in the field of biomedicine. Compared with traditional dyes and fluorescent proteins, quantum dots have unique optical properties, such as emission spectra that vary with size, high brightness, super photostability against photobleaching, and simultaneous excitation of multiple fluorescences. These characteristics make quantum dots and their conjugates a reliable optical marker, which has a wide range of applications in biological fields such as virus detection. [0003] Patent CN201210073965.4 provides a method for homogeneous immunodetection of various viruses based on quantum dots and graphene ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/576G01N33/551G01N33/548G01N33/545G01N33/58C08F251/02C08F220/56C08F222/38G01N21/64
CPCG01N33/56983G01N33/5761G01N33/54313G01N33/588C08B15/04C08F251/02G01N21/6428G01N2469/10C08F220/56C08F222/385
Inventor 李育修
Owner 苏州育德扬生物技术有限公司
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