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Biological material evidence collection and preservation reagent

A technology for preserving reagents and physical evidence, applied in the field of biological evidence collection and preservation reagents, can solve the problems of non-shedding cells and test materials peeling, corruption, etc., achieve high detection rate, improve detection rate, and inhibit nuclease to DNA the effect of destruction

Pending Publication Date: 2022-05-17
清远市公安局
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most commonly used reagent for moistening cotton swabs is deionized water, but studies have shown that biological samples extracted by wiping with deionized water are easily corrupted by microorganisms, resulting in the degradation of trace amounts of DNA
At present, experts and scholars in the market have added antibiotics to deionized water as exfoliated cell collection reagents. This method can effectively inhibit the damage of microorganisms to DNA, but it also has limitations, such as not being able to separate exfoliated cells from specimens and many more

Method used

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  • Biological material evidence collection and preservation reagent
  • Biological material evidence collection and preservation reagent
  • Biological material evidence collection and preservation reagent

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A configuration of biological evidence collection and preservation reagents, the reagents include sodium dodecylsulfonate (SDS), ethylenediaminetetraacetic acid (EDTA), trishydroxymethylaminomethane (Tris) and uric acid (Uric acid). In this reagent: the mass concentration of sodium dodecylsulfonate (SDS) is 10%, the molar concentration of ethylenediaminetetraacetic acid (EDTA) is 0.5mol / L, the molar concentration of trishydroxymethylaminomethane (Tris) The molar concentration of uric acid is 2mol / L.

[0024] The reagent of the present invention is (SDS-EDTA-Tris-uric acid) quadruple reagent, wherein, sodium dodecylsulfonate (SDS) belongs to anionic surfactant, has excellent penetration, washing, wetting, decontamination and emulsification , Soluble in water and ethanol, the special chemical structure can be similarly compatible with the cell membrane phospholipid bilayer, so it can destroy the cell membrane and release the DNA in it. Ethylenediaminetetraacetic acid (ED...

Embodiment 2

[0026] A kind of subpackaging, sealing, sterilization and nucleic acid-free treatment of biological evidence collection and preservation reagents (as described in Example 1), specifically as follows: the reagents with a pH value adjusted to 8.0 are subpackaged into nucleic acid-free reagent bottles, sealed , after high-pressure steam sterilization (temperature 121.3°C, pressure 103.4kPa, time 20min), take 100μl reagent to extract DNA by magnetic bead method, and use the kit to amplify, electrophoresis to detect STR typing, if not detected, it is qualified .

Embodiment 3

[0028] Fingerprint imprints were used to simulate the exfoliated cells at the crime scene, and the qualified reagent after treatment in Example 2 was compared with deionized water. After collecting 90 fingerprint exfoliated cells, the DNA was extracted by the magnetic bead method, amplified by a kit, and detected by electrophoresis for STR typing. In this experiment, more than 100 fluorescence units were used for interpretation. Statistics were not performed below this standard, and the calculation of two Detection rate of DNA loci in group experiments:

[0029] 90 fingerprints were collected from qualified reagents after treatment in Example 2, and the detection rate of DNA sites was 76.9%.

[0030] Deionized water was used to collect 90 fingerprints, and the detection rate of DNA sites was 42.9%.

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Abstract

The invention relates to the technical field of forensic physical evidence DNA extraction, and discloses a biological physical evidence collection and preservation reagent which comprises sodium dodecyl sulfate (SDS), ethylenediamine tetraacetic acid (EDTA), trihydroxymethyl aminomethane (Tris) and uric acid (Uric acid). In the reagent, the mass concentration of the sodium dodecyl sulfate (SDS) is 10%, the molar concentration of the ethylenediamine tetraacetic acid (EDTA) is 0.5 mol / L, the molar concentration of the trihydroxymethyl aminomethane (Tris) is 1 mol / L, and the molar concentration of the uric acid (Uric acid) is 2 mol / L. According to the reagent, four components (SDS-EDTA-Tris-uric acid) are combined and matched with one another, so that the reagent can be beneficial to stripping a biological material evidence from a detected material, damage of nuclease to DNA can be effectively inhibited, and a microenvironment for avoiding DNA degradation can be provided.

Description

technical field [0001] The invention relates to the technical field of forensic evidence DNA extraction, in particular to a biological evidence collection and preservation reagent. Background technique [0002] Forensic DNA technology has played an important role in the detection of many criminal cases, the resolution of civil cases, the identification of the source of corpses in major accidents, and the paternity testing of trafficked women and children. With the continuous improvement of the legal system and the continuous development of DNA technology, more and more cases and physical evidence require DNA testing. Forensic DNA analysis has become a routine technology for biological evidence testing. [0003] DNA is a macromolecule that exists in living organisms. In an in vitro environment, it is easily damaged, broken, and turned into small molecules, that is, DNA degradation. Successful DNA analysis begins with the correct extraction, storage and submission of biologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2527/125Y02A50/30
Inventor 吴雅兰陈灿球曹丹贾霄孙华明
Owner 清远市公安局