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Multi-walled carbon nanotube compound as well as preparation method and application thereof

A technology of multi-walled carbon nanotubes and composites, applied in biological testing, biological material analysis, material analysis using wave/particle radiation, etc., can solve problems such as personal injury, restrictions on non-professional workers, time-consuming and laborious problems, and achieve The effect of simple method

Active Publication Date: 2022-05-20
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that after the multi-walled carbon nanotubes are endowed with magnetism, more application functions have been added. However, these two traditional methods for preparing magnetic multi-walled carbon nanotubes are time-consuming and laborious, and often require high temperature conditions or stimulation. Corrosion and corrosive reagents, etc., corresponding protective measures should be taken during the test, otherwise misuse may cause personal injury
This is obviously not suitable for the development trend of green chemistry, and has certain restrictions on non-professional workers. Therefore, a simple, safe, convenient and adaptable effective compound method is of great significance

Method used

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  • Multi-walled carbon nanotube compound as well as preparation method and application thereof
  • Multi-walled carbon nanotube compound as well as preparation method and application thereof
  • Multi-walled carbon nanotube compound as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] The preparation of embodiment 1 multi-walled carbon nanotube composite

[0045] (1) Weigh an appropriate amount of carboxylated multi-walled carbon nanotubes and add them into a 0.1 mol / L MES (pH 4.0) glass bottle, prepare the multi-walled carbon nanotubes to a concentration of 1 mg / mL, shake and mix well.

[0046] (2) Add 200 μL each of 40 mmol / L EDC and NHS, mix well, place in a shaker at 25°C and 180 rpm, and react for 120 min.

[0047] (3) Add carbonyl iron powder at a ratio of 1:3 between multi-walled carbon nanotubes and carbonyl iron powder, mix well, and react in a shaker at 25°C and 180 rpm for 60 minutes to obtain the product.

Embodiment 2

[0048] The characterization of embodiment 2 multi-walled carbon nanotube composite

[0049] 1. Zeta potential analysis

[0050] The surface charges of multi-walled carbon nanotubes, carbonyl iron powder, and composites of multi-walled carbon nanotubes and carbonyl iron powder were analyzed by Malvern particle size analyzer. The detailed measurement steps are as follows: Clean the special zeta potential measurement cuvette of the Malvern particle size analyzer for 3 times; select the function of measuring zeta potential in the test software; add the sample to the specified scale of the cuvette, follow In the correct direction, put it in the corresponding place; the sample is a black solution, so the parameter is adjusted to the absorption rate for measurement; repeat the measurement 3 times, record the data and draw a graph for analysis.

[0051] Zeta potential is often used to achieve the characterization of the surface charge of nanoparticles. Analysis results such as ima...

Embodiment 3

[0058] Example 3 Construction of fluorescent aptasensor

[0059] (1) Weigh an appropriate amount of the multi-walled carbon nanotube composite prepared in Example 1, add it into a 2mL centrifuge tube filled with deionized water, prepare to a concentration of 1mg / mL, and disperse it ultrasonically for 60min.

[0060] (2) The multi-walled carbon nanotube complex solution after sonication was mixed with the Escherichia coli O157:H7 aptamer at a concentration of 100 nmol / L, and the ratio of the two was 1:1.

[0061] (3) The above mixture was ultrasonically dispersed for 60 min and then placed in a refrigerator at 4°C overnight.

[0062] (4) After separation by magnetic separation, wash with deionized water, and collect the resuspended solution to obtain the final product.

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Abstract

The invention discloses a multi-walled carbon nanotube compound as well as a preparation method and application thereof. The multi-walled carbon nanotube compound is mainly prepared from a multi-walled carbon nanotube and carbonyl iron powder. The invention also discloses a fluorescent aptamer sensor prepared from the multi-walled carbon nanotube compound and a preparation method of the fluorescent aptamer sensor. Compared with the prior art, the multi-walled carbon nanotube compound is synthesized by adopting a one-step method, the method is simple, safe and effective, and the obtained multi-walled carbon nanotube compound has magnetism, can be used for adsorbing a fluorescence-labeled nucleic acid aptamer and preparing a fluorescent aptamer sensor, and can be used for rapidly detecting escherichia coli, escherichia coli and the like. The kit is especially used for rapid detection of Escherichia coli O157: H7.

Description

technical field [0001] The invention belongs to the technical field of Escherichia coli detection, and in particular relates to a multi-walled carbon nanotube compound and its preparation method and application. Background technique [0002] Due to the special structure, surface functional group modification and π-bond stacking, multi-walled carbon nanotubes can link biomolecules such as nucleic acid aptamers with different targeting functions under covalent or non-covalent interactions. Gu et al. used polyethylene glycol as an adhesive to covalently link hydroxylated multi-walled carbon nanotubes to amino-modified nucleic acid aptamers for the diagnosis of prostate cancer, which was verified in both cell and animal experiments. . Barbosa et al. used ultrasound as the main treatment method to non-covalently link multi-walled carbon nanotubes and nucleic acid aptamers for the detection of human colon cancer cells, and obtained good results in cytotoxicity tests. Although a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/533G01N33/58G01N21/64G01N21/3563G01N23/2273
CPCG01N33/56916G01N33/533G01N33/582G01N21/6428G01N21/3563G01N23/2273G01N2333/245G01N2021/6432G01N2021/6439G01N2021/3595Y02A50/30
Inventor 庞立冬姜毓君满朝新杨鑫焱
Owner NORTHEAST AGRICULTURAL UNIVERSITY