Method for synthesizing basic nickel carbonate based on biomineralization reaction
A technology of biomineralization and nickel carbonate, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high temperature and time requirements, difficult removal of harmful impurities, complicated post-processing steps, etc. Achieve the effect of simple steps, mild conditions and mild reaction conditions
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Embodiment 1
[0033] A method for synthesizing basic nickel carbonate based on biomineralization reaction is carried out according to the following steps successively:
[0034] 1. In a sterile environment, firstly inoculate Bacillus Pasteurella into LB solid medium supplemented with urea by streaking on a plate, culture at 37°C for 36h to obtain a plate colony, and then pick a single colony and inoculate it to In 3ml of LB liquid medium supplemented with urea, incubate at 30°C for 24h on a shaker, then inoculate the bacterial liquid into the LB liquid medium at a volume ratio of 1:100, and incubate again at 30°C for 24h on a shaker. , the formula of the LB solid medium added with urea is: sodium chloride 10g / L, tryptone 10g / L, yeast powder 5g / L, urea 10g / L, agar 20g / L, the described urea added The formula of LB liquid medium is: sodium chloride 10g / L, tryptone 10g / L, yeast powder 5g / L, urea 10g / L;
[0035] 2. Centrifuge the incubated bacterial solution at 4°C and take the precipitate, then...
Embodiment 2
[0039] The difference from Example 1 is:
[0040] In step 1, the formula of the LB solid medium added with urea is: sodium chloride 9g / L, tryptone 9g / L, yeast powder 4.5g / L, urea 9g / L, agar 18g / L, the The formula of the LB liquid medium supplemented with urea is: sodium chloride 9g / L, tryptone 9g / L, yeast powder 4.5g / L, urea 9g / L;
[0041] In step 2, the OD value of the resuspended bacterial solution is 3.2;
[0042] In step 3, the volume ratio of the resuspended bacterial solution to the mixed solution of urea and nickel chloride is 1:1.2, and the time for standing is 20min.
Embodiment 3
[0044] The difference from Example 1 is:
[0045] In step 3, the resuspended bacterial solution and the mixed solution of urea and nickel chloride are mixed in equal volumes at room temperature, and sodium hydroxide solution is rapidly added dropwise, and the pH of the system is adjusted to 8.0, so that the biologically induced mineralization reaction is carried out in an alkaline environment, The precipitate obtained by centrifugation is washed, dried in a hot blast vacuum drying oven at 80°C, and then ground into fine particles with a mortar, and the product is green.
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