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Nanoparticle composite hydrogel nerve conduit and preparation method thereof

A composite hydrogel and nanoparticle technology, applied in pharmaceutical formulations, prostheses, drug delivery, etc., can solve the problems of fast degradation rate, poor mechanical properties, etc., to promote elongation, meet mechanical requirements, and good biocompatibility sexual effect

Active Publication Date: 2022-05-27
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult for a single material to meet all the above characteristics at the same time. It is the current development trend to use multiple materials with different advantages to construct composite artificial nerve guides.
[0004] Gelatin is a further hydrolysis product of collagen, which retains some collagen signal sequences such as RGD, which can better support cell adhesion and growth, but due to its degradation rate Fast and poor mechanical properties are difficult to apply to neural tissue engineering

Method used

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  • Nanoparticle composite hydrogel nerve conduit and preparation method thereof
  • Nanoparticle composite hydrogel nerve conduit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Dissolve miRNA-29a in DEPC water at a concentration of 110 μg / ml, then add ZIF-8 suspension to ZIF-8 at a concentration of 1 mg / ml, with a mass ratio of miRNA-29a to ZIF-8 of 2:100. The mixture is then incubated on a shaker at 500 rpm / min for 30 min. Finally, the solution was centrifuged at 12,000 rpm for 10 min to collect the pellet to obtain miRNA-29a@ZIF-8 nanoparticles.

[0029] 2) Gelatin dissolved in a buffered aqueous solution of 2-ethionisulfonic acid at a concentration of 0.02g / mL, followed by 0.01g / ml of tyramine hydrochloride, fully dissolved at 50 °C, after the solution was cooled to room temperature, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinateimide (0.37g / 0.11 g) were added, stirring reaction for 12h, after dialysis under deionized water environment for 4 days, lyophilization was given to obtain sponge gelatin modified products, It is tyramine-modified gelatin.

[0030] 3) Weigh 4g of secondary degummed silk and fully di...

Embodiment 2

[0033] 1) Dissolve miRNA-29a in DEPC water at a concentration of 110 μg / ml, then add ZIF-8 suspension to ZIF-8 at a concentration of 3 mg / ml, with a mass ratio of miRNA-29a to ZIF-8 of 6:100. Subsequently incubate the mixture on a shaker at 500 rpm for 30 min. Finally, the solution was centrifuged at 12,000 rpm for 10 min to collect the pellet to obtain miRNA-29a@ZIF-8 nanoparticles.

[0034]2) Gelatin dissolved in a 50mM 2-equinethanesulfonic acid buffered aqueous solution at a concentration of 0.02g / mL, followed by 0.01g / mL of tyramine hydrochloride, fully dissolved at 50 °C, after the solution was cooled to room temperature, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinateimide (0.37g / 0.11 g) were added, stirring reaction for 12h, dialysis in deionized water environment for 4 days, lyophilization was given to obtain sponge gelatin modified products, It is tyramine-modified gelatin.

[0035] 3) Weigh 4g of secondary degummed silk and fully disso...

Embodiment 3

[0038] 1) Dissolve miRNA-29a in DEPC water at a concentration of 110 μg / ml, then add ZIF-8 suspension to ZIF-8 at a concentration of 1.5 mg / ml, with a mass ratio of miRNA-29a to ZIF-8 of 4:100. Subsequently incubate the mixture on a shaker at 500 rpm for 30 min. Finally, the solution was centrifuged at 12,000 rpm for 10 min to collect the pellet to obtain miRNA-29a@ZIF-8 nanoparticles. The stability of miRNA-29a@ZIF-8 is judged by the change of its electrophoretic bands with the incubation time in serum, such as Figure 2 as shown.

[0039]2) Gelatin dissolved in a buffered aqueous solution of 2-malineacesulfonic acid at a concentration of 0.02g / ml, followed by 0.01g / ml of tyramine hydrochloride, dissolved sufficiently at 50 °C, after the solution was cooled to room temperature, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (0.37g / 0.11 g) were added, stirred reaction for 12h, after dialysis under deionized water environment for 4 days, lyophil...

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Abstract

The invention discloses a nanoparticle composite hydrogel nerve conduit and a preparation method thereof. The preparation method comprises the following steps: 1) preparing miRNA-29a ZIF-8 nanoparticles, a silk fibroin solution and tyramine modified gelatin; 2) dispersing miRNA-29a ZIF-8 nanoparticles in a silk fibroin solution, dissolving tyramine modified gelatin in the silk fibroin solution, uniformly mixing and stirring the two solutions, and adding horse radish peroxidase to obtain a mixed solution; and injecting the mixed solution into a tubing mold, placing the tubing mold in a refrigerator at a specific temperature, after forming thermally reversible hydrogel, pushing out the thermally reversible hydrogel, soaking the thermally reversible hydrogel in hydrogen peroxide, crosslinking for a period of time, taking out the thermally reversible hydrogel, cutting off two ends of the conduit, and washing off non-crosslinked parts to obtain the nano-particle composite hydrogel nerve conduit. The nerve conduit prepared by the method has excellent biocompatibility and meets the mechanical requirements of the nerve conduit, and the released miRNA-29a and Zn < 2 + > can accelerate the nerve repair process, so that the peripheral nerve injury can be better treated.

Description

Technical field [0001] The present invention relates to the technical field of biomedical materials, in particular refers to a nanoparticle composite hydrogel neural catheter and preparation method thereof. Background [0002] Due to the wide distribution of peripheral nerves in the human body, tissue damage caused by accidents and the like is often accompanied by peripheral nerve defects. Once a peripheral nerve defect occurs, it is difficult to achieve self-repair, and commonly used clinical treatment methods such as suture of the broken end, autologous or allogeneic nerve transplantation are currently facing various problems, such as difficulty in repairing long-distance nerve defects and insufficient donor sources, as well as ethical restrictions. In recent years, the research and development of artificial neural catheters has provided new ideas for the repair of peripheral nerve defects. The biocompatible, bioactive, and completely degradable artificial neural catheter can p...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/02A61L27/50A61L27/52A61L27/54A61L27/58
CPCA61L27/222A61L27/227A61L27/025A61L27/50A61L27/52A61L27/54A61L27/58A61L2400/12A61L2430/32A61L2300/258A61L2300/412C08L89/00
Inventor 高会场曹晓东王浩
Owner SOUTH CHINA UNIV OF TECH
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