Method for biomineralization treatment of chemical nickel plating wastewater

A technology of electroless nickel plating and biomineralization, applied in biochemical equipment and methods, methods based on microorganisms, sustainable biological treatment, etc., can solve the problems of ineffective recovery of sedimentation products, rational use of unfavorable resources, etc., and achieve high removal The effect of high rate, fast removal rate and high removal efficiency

Pending Publication Date: 2022-06-07
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the method of treating heavy metals in sewage through microbial mineralization mostly adopts the method of solidification a

Method used

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  • Method for biomineralization treatment of chemical nickel plating wastewater
  • Method for biomineralization treatment of chemical nickel plating wastewater
  • Method for biomineralization treatment of chemical nickel plating wastewater

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for biomineralization treatment of chemical nickel plating wastewater, the following steps are followed in turn:

[0029] 1. In a sterile environment, firstly inoculate Bacillus Pasteurella into LB solid medium supplemented with urea by streaking on a plate, and culture at 37°C for 36 hours to obtain plate colonies, and then pick out the surrounding color that turns dark red. A single colony was inoculated into 3ml of LB liquid medium supplemented with urea, incubated at 30°C and 220rpm for 24h on a shaker, then the bacterial liquid was inoculated into LB liquid medium at a volume ratio of 1:100, and again at 30°C Shaker was incubated for 24h to obtain a bacterial liquid with an OD value of 3.2, wherein the formula of the LB solid medium added with urea was: sodium chloride 10g / L, tryptone 10g / L, yeast powder 5g / L, urea 10g / L, agar 20g / L, phenol red 0.05g / L, the formula of the LB liquid medium added with urea is: sodium chloride 10g / L, tryptone 10g / L, yeast pow...

Embodiment 2

[0032] The difference from Example 1 is:

[0033] In step 2, before adding the bacterial solution to the mixed solution of urea and nickel chloride, resuspend it, specifically:

[0034] The bacterial solution was first centrifuged at 4°C and the precipitate was taken, then washed once with sterile physiological saline and twice with sterile water, and finally added sterile water to resuspend, so that the volume of bacterial solution before and after resuspension was the same , wherein the OD value of the resuspended bacterial solution was 3.0.

Embodiment 3

[0036] The difference from Example 1 is:

[0037] In step 2, the present embodiment replaces the mixed solution of urea and nickel chloride in embodiment 1 with the mixed solution of urea and electroless nickel plating wastewater, and the concentration of urea in the mixed solution is set with embodiment 1, and the concentration of nickel ion is 28.93mg / L.

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Abstract

The invention relates to a method for biomineralization treatment of chemical nickel plating wastewater, which comprises the following steps: culturing sporosarcina pasteurii to obtain a bacterial solution, adding the bacterial solution into a mixed solution of urea and chemical nickel plating wastewater according to a specific ratio, carrying out a biomineralization reaction at room temperature, collecting mineralized substances after the reaction is completed, and sequentially washing and drying to obtain nickel carbonate precipitate. According to the design, the nickel ion removal efficiency is high, the by-product nickel carbonate can be used as a ceramic coloring agent to prepare nickel oxide or organic nickel salt, and living bacteria can be recycled.

Description

technical field [0001] The invention belongs to the technical field of microbial induced mineralization, and in particular relates to a method for biomineralization treatment of chemical nickel plating wastewater. Background technique [0002] Industrial wastewater is the wastewater and waste liquid discharged from the process of production, which contains a variety of inorganic and organic toxic substances related to raw materials, among which the money printing wastewater is representative. Its chromaticity is large, the pollutant concentration is high, and the composition is complex, including plating waste water, cleaning waste liquid and cleaning waste water. Among them, chemical nickel plating waste water contains a large amount of nickel ions, ranging from tens to hundreds of milligrams per liter. [0003] The treatment methods of electroless nickel plating wastewater can usually be divided into three categories: physical method, chemical method and bioremediation met...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F3/00C01G53/06C12N1/20C02F103/16C02F101/20C02F101/10C12R1/07
CPCC02F3/34C02F3/348C02F3/342C02F3/005C01G53/06C12N1/20C02F2103/16C02F2209/08C02F2209/06C02F2101/20C02F2101/10C02F1/286C02F2305/06Y02W10/10
Inventor 李其昌段锋谢浩郭君慧徐立贾明宇王霄晗冯小粟
Owner WUHAN UNIV OF TECH
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