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Method for detecting adsorption efficiency of medicine on nucleic acid metabolite and application of method

A technology of adsorption efficiency and metabolites, which is applied in the field of biomedicine, can solve the problems of low accuracy and lack of adsorption capacity methods, and achieve the effects of high accuracy, saving detection costs, and improving detection efficiency

Pending Publication Date: 2022-06-17
WATERSTONE PHARMA WUHAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The market for polymer drugs that adsorb nucleotide metabolites is lacking, and the methods to detect the adsorption capacity of these drugs to nucleotide metabolites are also lacking, and the accuracy is not high

Method used

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  • Method for detecting adsorption efficiency of medicine on nucleic acid metabolite and application of method
  • Method for detecting adsorption efficiency of medicine on nucleic acid metabolite and application of method
  • Method for detecting adsorption efficiency of medicine on nucleic acid metabolite and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0098] Example 2 Column temperature 25°C

[0099] Instrument: Agilent 1260 high performance liquid chromatograph, 1260 Infinity diode array detector

[0100] Chromatographic column: YMC-Pack ODS-AQ C18 (150×4.6mm, 5μm)

[0101] Mobile phase A: 0.05mol / L potassium dihydrogen phosphate buffer (adjust pH to 4.0 with phosphoric acid or sodium hydroxide test solution)

[0102] Mobile Phase B: Acetonitrile

[0103] Gradient elution is the same as in Example 1

[0104] Flow rate: 0.6mL / min

[0105] Detection wavelength: 260nm

[0106] Column temperature: 25℃

[0107] Injection volume: 10 μL

[0108] Diluent: potassium dihydrogen phosphate buffer (pH 6.8)

[0109] Run time: 60 minutes

[0110] Experimental steps:

[0111] Potassium dihydrogen phosphate buffer (pH 6.8): Weigh 13.658 g of potassium dihydrogen phosphate, put it in a 1000 mL volumetric flask, add water to dissolve and dilute to the mark, and adjust the pH to 6.8 with phosphoric acid or sodium hydroxide test solut...

Embodiment 3

[0116] Example 3 Column temperature 35°C

[0117] Instrument: Agilent 1260 high performance liquid chromatograph, 1260 Infinity diode array detector

[0118] Chromatographic column: YMC-Pack ODS-AQ C18 (150×4.6mm, 5μm)

[0119] Mobile phase A: 0.05mol / L potassium dihydrogen phosphate buffer (adjust pH to 4.0 with phosphoric acid or sodium hydroxide test solution)

[0120] Mobile Phase B: Acetonitrile

[0121] Gradient elution is the same as in Example 1

[0122] Flow rate: 0.6mL / min

[0123] Detection wavelength: 260nm

[0124] Column temperature: 35℃

[0125] Injection volume: 10 μL

[0126] Diluent: potassium dihydrogen phosphate buffer (pH 6.8)

[0127] Run time: 60 minutes

[0128] Experimental steps:

[0129] Potassium dihydrogen phosphate buffer (pH 6.8): Weigh 13.658 g of potassium dihydrogen phosphate, put it in a 1000 mL volumetric flask, add water to dissolve and dilute to the mark, and adjust the pH to 6.8 with phosphoric acid or sodium hydroxide test solut...

Embodiment 4

[0134] Embodiment 4 buffer reagent is BES

[0135] Instrument: Agilent 1260 high performance liquid chromatograph, 1260 Infinity diode array detector

[0136] Chromatographic column: YMC-Pack ODS-AQ C18 (150×4.6mm, 5μm)

[0137] Mobile phase A: 0.05mol / L potassium dihydrogen phosphate buffer (adjust pH to 4.0 with phosphoric acid or sodium hydroxide test solution)

[0138] Mobile Phase B: Acetonitrile

[0139] Gradient elution is the same as in Example 1

[0140] Flow rate: 0.6mL / min

[0141] Detection wavelength: 260nm

[0142] Column temperature: 30℃

[0143] Injection volume: 10 μL

[0144] Diluent: BES buffer (pH 7.0)

[0145] Run time: 60 minutes

[0146] Experimental steps:

[0147] BES buffer (pH7.0): Weigh 21.32g of BES (N,N-(2-hydroxyethyl)-2-aminoethanesulfonic acid) and 4.67g of sodium chloride, add them to 1000ml of purified water, dissolve, mix Evenly, adjust the pH value to 7.0 with saturated sodium hydroxide solution (about 3.5ml).

[0148] Preparatio...

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Abstract

The invention provides a method for detecting the adsorption efficiency of a drug on nucleic acid metabolites. The method comprises the following steps: mixing a buffer solution containing nucleic acid metabolites with a medicine to obtain a test solution; the test solution is subjected to high performance liquid chromatography detection, the adsorption efficiency of the medicine to nucleic acid metabolites is determined based on the detection result, and the nucleic acid metabolites comprise at least one selected from uric acid, adenine, guanine, hypoxanthine, adenine nucleoside, guanine nucleoside, inosine and 5-adenosine monophosphate; a mobile phase adopted by the high performance liquid chromatography detection comprises a buffer solution and / or an organic solvent, and the pH value of the buffer solution is 3.6-4.0.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular, to a method for detecting the adsorption efficiency of drugs to nucleic acid metabolites and applications thereof, and more particularly, to a method for detecting the adsorption efficiency of drugs to nucleic acid metabolites and a method for screening drugs. Background technique [0002] "2019 Guidelines for Diagnosis and Treatment of Hyperuricemia and Gout in China" pointed out that hyperuricemia is a metabolic abnormal syndrome caused by purine metabolism disorder. Hyperuricemia (HUA) is defined as hyperuricemia when fasting blood uric acid levels are higher than 420 μmol / L in men and 360 μmol / L in women on two different days under a normal purine diet. When blood uric acid exceeds its saturation in blood or tissue fluid, uric acid crystals can be formed and deposited locally in the joints, causing local inflammatory response and tissue destruction, namely gout; deposition in...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/74G01N30/86G01N30/88
CPCG01N30/02G01N30/34G01N30/74G01N30/8634G01N30/8679G01N30/88G01N2030/8827G01N2030/884
Inventor 李彤彤梁樱郭丽娟刘燕鸣
Owner WATERSTONE PHARMA WUHAN
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