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Lactobacillus paracasei and application of lactobacillus paracasei in preparation of product for regulating skin microecosystem

A technology of micro-ecosystem, Lactobacillus, applied in the field of microbiology

Inactive Publication Date: 2022-06-21
梁天晓
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lactobacillus paracasei has great benefits in human nutrition and gastrointestinal health, but there are few reports on the application of Lactobacillus paracasei in regulating skin microecology

Method used

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  • Lactobacillus paracasei and application of lactobacillus paracasei in preparation of product for regulating skin microecosystem
  • Lactobacillus paracasei and application of lactobacillus paracasei in preparation of product for regulating skin microecosystem
  • Lactobacillus paracasei and application of lactobacillus paracasei in preparation of product for regulating skin microecosystem

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Separation of GforU-7

[0035] Sampling from feces of a 3-year-old healthy girl. After the samples were properly treated, they were shaken and mixed in normal saline. The supernatant was taken and streaked on the MRS solid plate. After 48 hours of constant temperature incubation at 37°C, white colonies were picked and repeatedly inoculated and screened until a uniform single colony was obtained, which was named GforU-7. .

[0036] Gram stain microscopy: Strain GforU-7 is Gram stain positive and is rod-shaped under the microscope; it grows on MRS plates and can form small white, round and opaque small colonies with a smooth surface and neat edges; cultured in MRS liquid In the base, the growth is uniform and turbid, and the bacteria are white precipitated after being left for a long time.

Embodiment 2

[0037] Nucleic acid identification of embodiment 2GforU-7

[0038] 1. 16s rRNA gene sequence analysis:

[0039] A single colony was picked and placed in MRS liquid medium, and after culturing overnight at 37°C, the cells were collected by centrifugation, and the sequences of the cells were amplified according to the steps of the DNA extraction kit. The primers were bacterial universal primers 27F and 1492R; the PCR amplification system was a 50 μL system, and the PCR amplification program was: 95°C pre-denaturation for 5 min; 94°C for 15s, 57°C for 15s, 72°C for 40s, 35 cycles; 72°C for 10min extension. After amplification, the base sequence of the nucleic acid is determined.

[0040] 2. Results

[0041] The homology comparison between the sequencing results of PCR products and the published standard sequences in GenBank (BLASTN) concluded that the GforU-7 strain was Lactobacillus paracasei.

Embodiment 3

[0042]Example 3: GforU-7 reduces NO production in Raw264.7 cells

[0043] 1. Preparation of GforU-7 bacterial solution

[0044] GforU-7 was cultured in MRS medium overnight, OD600 was detected, and the concentration of bacterial liquid was adjusted to OD600=0.2. After centrifugation, the cells were autoclaved at 121 °C for 30 min to obtain GforU-7 inactivated cells, and the centrifugal fermentation broth was filtered with 0.22 μm. The GforU-7 supernatant was obtained by membrane filtration.

[0045] 2. Preparation of Raw264.7 cells

[0046] Raw264.7 cells were digested at 2 × 10 5 The cells / well were inoculated into a 24-well plate, and cultured overnight at 37°C in a 5% carbon dioxide incubator.

[0047] 3. GforU-7 addition and LPS stimulation

[0048] The supernatant was added to the Raw264.7 cells cultured overnight in different groups at a ratio of 5% (V / V) and 10% (V / V) of inactivated bacteria, and 0.5ml was added after 2h at a concentration of 0.2μg / ml LPS solution...

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Abstract

The invention relates to the technical field of microorganisms, in particular to lactobacillus paracasei and application of the lactobacillus paracasei in preparation of a product for regulating a skin microecosystem. According to the invention, the lactobacillus paracasei GforU-7 strain is separated from feces of 3-year-old healthy girls, and has the effect of regulating the skin micro-ecology; comprising the following steps: reducing the relative proportion of propionibacterium acnes to staphylococcus epidermidis, reducing the relative proportion of staphylococcus aureus to staphylococcus epidermidis, inhibiting the growth of bromhidrosis pathogenic bacteria (including staphylococcus aureus, staphylococcus epidermidis, staphylococcus hominis, staphylococcus haemolyticus and corynebacterium drying), and inhibiting the bromhidrosis pathogenic bacteria from producing isovaleric acid; the NO release amount is reduced, and / or the level of inflammatory factors IL-8 and / or TRPV1 is reduced, and the expression of barrier repair related genes FLG and / or LOR is up-regulated. The lactobacillus paracasei GforU-7 strain provided by the invention has great market potential in the fields of food, medicines, skin care products, cosmetics and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Lactobacillus paracasei and its application in preparing a product for regulating skin micro-ecosystem. Background technique [0002] As the largest organ of the human body, the skin is in direct contact with the external environment and is the first barrier to protect the human body. At the same time, there are a large number of bacteria accumulated on the skin surface, there are about 1000 species, which can be mainly divided into four categories, including Actinomycetes (51.8%), Firmicutes (24.4%), Proteobacteria (16.5%) ) and Bacteroidetes (6.3%), while the established genera were Corynebacterium, Propionibacterium and Staphylococcus. The skin microbiota can resist the adhesion and development of skin pathogens, and can also talk to the immune system, so the imbalance of the skin microecological system can cause various skin diseases, and conversely, skin ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61K8/99A61P17/10A61P17/00A61P31/04A61Q15/00A61Q17/00C12R1/225
CPCA61K35/747A61P17/00A61P17/10A61P31/04A61K8/99A61Q17/005A61Q15/00
Inventor 梁天晓
Owner 梁天晓
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