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Hydrophilic negatively-charged porous nano-film for repairing chronic kidney disease as well as preparation method and application of hydrophilic negatively-charged porous nano-film

A nano-membrane and chronic kidney disease technology, applied in prosthetics, tissue regeneration, medical science, etc., can solve the problems of complex preparation methods, easy to cause thrombosis, etc., achieve simple and clear routes, improve hydrophilicity, and enhance biocompatibility Effect

Pending Publication Date: 2022-06-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN103933615A discloses a self-assembled porous polymer membrane for glomerular filtration membrane repair, but its preparation method is complicated, and the surface is hydrophobic, which is easy to cause thrombus

Method used

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  • Hydrophilic negatively-charged porous nano-film for repairing chronic kidney disease as well as preparation method and application of hydrophilic negatively-charged porous nano-film
  • Hydrophilic negatively-charged porous nano-film for repairing chronic kidney disease as well as preparation method and application of hydrophilic negatively-charged porous nano-film
  • Hydrophilic negatively-charged porous nano-film for repairing chronic kidney disease as well as preparation method and application of hydrophilic negatively-charged porous nano-film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) 0.125 mmol of sucrose and 0.1 mol of L-lactide were added to the round-bottomed flask, and 1 mmol of the catalyst stannous isooctanoate was added, and nitrogen gas was passed for 40 minutes to remove the air in the reaction flask, and then sealed, at 100 The reaction was stirred at ℃ for 24 hours to obtain 8-arm star-shaped polylactic acid (G 1- (OH) 8 );

[0042] (2) G is obtained by applying the above 1- (OH) 8 Dissolve with dichloromethane and precipitate with methanol, respectively, and collect the product by suction filtration. After repeating 3 times, vacuum drying at 45 °C for 8 hours to obtain G 1- (OH) 8 White powder;

[0043] (3) 1 mmol of G 1- (OH) 8 The white powder was dissolved in anhydrous 1,4-dioxane, and 2 mmol of methacrylic anhydride, 2 mmol of 4-dimethylaminopyridine and 2 mmol of 3-ethylamine were added, and the reaction was vigorously stirred at room temperature for 24 hours. , to obtain an 8-arm star-shaped polylactic acid (G 2 -(meth...

Embodiment 2

[0052] Podocytes (purchased from ATCC, USA) were grown to at least 80% confluent, digested with trypsin, and made into 5*10 4 100ul / ml of cell suspension, planted in a 96-well plate for 24 hours, replaced with serum-free basal medium for starvation for 6 hours to synchronize the cell state, and used the basal medium to target the podocytes prepared in Example 1. The hydrophilic and negatively charged porous nano-films are configured into different concentrations (0.1mg / ml, 1mg / ml, 2mg / ml, 5mg / ml, 10mg / ml), drop 10ul into a 96-well plate, and set 3 duplicate wells for each concentration . At the same time, a normal control group without material intervention and a cell-free basal medium blank group were added for comparison. After culturing cells in each well for 24 hours, 10ul of cck-8 reagent was added dropwise, and after 1 hour, the absorbance at 450 nm wavelength was measured with a microplate reader, and the experiment was repeated three times. see the results image 3 ...

Embodiment 3

[0054] The BALB / c mice were accurately weighed and randomly divided into 3 groups, namely the control group, the unmodified PEG negatively charged porous nanofilm group (prepared according to the step (7) of Example 1) and the modified PEG micelles of Example 1. Cell-targeted hydrophilic negatively charged porous nanofilm group, 5 mice in each group, mice were injected with saline, unmodified PEG negatively charged porous nanofilm (30 mg / kg) and modified PEG negatively charged porous nanofilm through tail vein respectively Nanofilm (30mg / kg). All the mice in the unmodified PEG group had various degrees of wheezing and suffocation within one minute after injection, indicating pulmonary thrombosis; the vital signs of the mice in the control group and the modified PEG group were normal, and all the mice were sacrificed immediately. The lungs and livers were collected, and H&E sections of the organs were prepared. Finally, the pathological changes of the organs were observed under...

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Abstract

The invention discloses a hydrophilic negatively-charged porous nano-film for repairing chronic kidney diseases as well as a preparation method and application of the hydrophilic negatively-charged porous nano-film. According to the method, cane sugar and L-lactide are taken as reactants, a terminal-carboxylated 8-arm star-shaped polylactic acid element is synthesized, and the negative-electricity porous nano-film with the pore diameter of 23.5 + / -4.5 nm is prepared from the element through self-assembly; the surface of the film is successfully modified with polyethylene glycol (PEG) and a glomerular specific antibody (Ab) in sequence by utilizing an amide reaction between carboxyl and amino, so that the film has good hydrophilicity and kidney targeting property; the pore diameter of the hydrophilic negative-electricity porous nano-film for repairing the chronic kidney disease is 19-28 nm. The hydrophilic negatively-charged porous nano-film prepared by the invention can simulate a filtration barrier of glomerulus and directly repair a damaged glomerulus filtration film of a rat, so that the effect of treating chronic kidney disease is achieved.

Description

technical field [0001] The invention belongs to the field of biological functional material preparation technology, and in particular relates to a hydrophilic negatively charged porous nano film used for the repair of chronic kidney disease, and a preparation method and application thereof. Background technique [0002] With the increase of people's work and life pressure and the increase of bad living habits, the incidence of chronic kidney disease in the world is increasing year by year, and it has become one of the "global public health problems". When chronic kidney disease progresses to the end-stage renal failure, patients can only rely on hemodialysis or even kidney transplantation to maintain their lives, which also means that patients need to pay expensive treatment costs. Studies have shown that the underlying cause of chronic kidney disease is a decrease in the glomerular filtration rate caused by glomerular damage. Among them, the glomerular filtration barrier u...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/54A61L27/22A61L27/18C08G63/08C08G63/91
CPCA61L27/56A61L27/54A61L27/227A61L27/18C08G63/08C08G63/912A61L2430/26C08L89/00C08L71/02C08L67/04
Inventor 胡建强伍倩清曾涛
Owner SOUTH CHINA UNIV OF TECH
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