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Porous three-dimensional gelatin microsphere and preparation method thereof

A technology of three-dimensional gelatin and microspheres, applied in the field of gelatin, can solve the problems of reducing drug-carrying effect, poor swelling degree of microspheres, easy occurrence of tailing of microspheres, etc.

Pending Publication Date: 2022-06-24
罗赛洛(温州)明胶有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, not only the pore size is not easy to control, but also microspheres are prone to tailing during the stirring process, resulting in small roundness of the microspheres, large particle size distribution, poor swelling of the microspheres, and reduced drug loading effect.

Method used

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  • Porous three-dimensional gelatin microsphere and preparation method thereof
  • Porous three-dimensional gelatin microsphere and preparation method thereof
  • Porous three-dimensional gelatin microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of porous three-dimensional gelatin microspheres, comprising the following steps:

[0030] S1. Add 1kg of chitosan with a degree of deacetylation of 55% into 5kg of 0.8% acetic acid aqueous solution and stir evenly, add 1kg of polyvinyl alcohol under stirring, and continue to stir for 1 h to obtain a polyvinyl alcohol / chitosan mixed solution 1kg nano calcium carbonate is added in 5kg ethanol and stir to obtain pretreated nano calcium carbonate;

[0031] Add 10kg of gelatin to 50kg of water, dissolve completely at 40°C, add 1kg of pretreated nano-calcium carbonate and 8kg of polyvinyl alcohol / chitosan mixed solution under stirring to obtain pretreated gelatin;

[0032] S2, add 1kg of Span-80 and 1kg of Tween-60 to 200kg of liquid paraffin and stir evenly, add 20kg of pretreated gelatin under stirring at 40°C, continue stirring for 10min, with a stirring speed of 3000r / min, cool down to room temperature, add 1kg The glutaraldehyde solution with a mas...

Embodiment 2

[0035] A preparation method of porous three-dimensional gelatin microspheres, comprising the following steps:

[0036] S1, add 5kg of chitosan with a degree of deacetylation of 70% into 10kg of 1.2% acetic acid aqueous solution and stir evenly, add 3kg of polyvinyl alcohol under stirring, and continue to stir for 2h to obtain a polyvinyl alcohol / chitosan mixed solution 5kg nano calcium carbonate is added in 10kg ethanol and stir to obtain pretreated nano calcium carbonate;

[0037] Add 20kg of gelatin into 100kg of water, dissolve completely at 60°C, add 5kg of pretreated nano-calcium carbonate and 18kg of polyvinyl alcohol / chitosan mixed solution under stirring to obtain pretreated gelatin;

[0038] S2, add 2kg Span-80 and 2kg Tween-60 into 400kg liquid paraffin and stir evenly, add 40kg pre-treated gelatin under stirring at 50°C, continue stirring for 20min, the stirring speed is 5000r / min, cool down to room temperature, add 2kg The glutaraldehyde solution with a mass fract...

Embodiment 3

[0041] A preparation method of porous three-dimensional gelatin microspheres, comprising the following steps:

[0042] S1. Add 2kg of chitosan with a degree of deacetylation of 66% into 7kg of 1.1% acetic acid aqueous solution and stir evenly, add 1.5kg of polyvinyl alcohol under stirring, and continue to stir for 1.7h to obtain polyvinyl alcohol / chitosan Mixed solution; 2kg nano calcium carbonate is added in 9kg ethanol and stirred to obtain pretreated nano calcium carbonate;

[0043] Add 12kg of gelatin to 90kg of water, dissolve completely at 45°C, add 4kg of pretreated nano-calcium carbonate and 10kg of polyvinyl alcohol / chitosan mixed solution under stirring to obtain pretreated gelatin;

[0044] S2, add 1.7kg Span-80 and 1.4kg Tween-60 to 340kg liquid paraffin and stir evenly, add 35kg pretreated gelatin under stirring at 42°C, continue stirring for 12min, the stirring speed is 4500r / min, and the temperature is lowered to room temperature, Add 1.3kg of glutaraldehyde so...

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Abstract

The invention discloses a porous three-dimensional gelatin microsphere preparation method, which comprises: adding chitosan into an acetic acid aqueous solution, uniformly stirring, adding polyvinyl alcohol under the stirring state, and continuously stirring for 1-2 h to obtain a polyvinyl alcohol / chitosan mixed solution; adding nano calcium carbonate into ethanol, and uniformly stirring to obtain pretreated nano calcium carbonate; adding gelatin into water, completely dissolving at 40-60 DEG C, adding the pretreated nano calcium carbonate and the polyvinyl alcohol / chitosan mixed solution in a stirring state, and uniformly mixing to obtain pretreated gelatin; adding span-80 and tween-60 into liquid paraffin, uniformly stirring, adding the pretreated gelatin at 40-50 DEG C in a stirring state, continuously stirring for 10-20 minutes, cooling to room temperature, adding a glutaraldehyde solution, and continuously stirring to obtain cross-linked gelatin; and standing the cross-linked gelatin, pouring out the supernate, removing the liquid paraffin, washing, soaking in a citric acid solution, stirring, and carrying out low-temperature vacuum drying to obtain the porous three-dimensional gelatin microspheres.

Description

technical field [0001] The invention relates to the technical field of gelatin, in particular to a porous three-dimensional gelatin microsphere and a preparation method thereof. Background technique [0002] Gelatin is the main product of animal collagen after mild and irreversible cleavage. It is a functional biopolymer material widely used in the fields of medicine, food, tissue engineering and agriculture. It has good biodegradability and biocompatibility. Its main amino acids are glycine, alanine and proline, which account for 67% of the total mass of gelatin amino acids. [0003] The characteristic of gelatin is that it can be reversibly transformed between gel and sol. The carboxyl and amino groups of gelatin molecules are polar groups with high affinity for water, while the affinity between linear long-chain molecules is large, so gelatin is linear in aqueous solution. The macromolecule hosts are closely attached to each other. Gelatin and dialdehydes are prone to c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/14
CPCB01J13/14Y02A50/30
Inventor 苏铭晖李传算蔡礼昊袁佳郑远领
Owner 罗赛洛(温州)明胶有限公司