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Molecular marker of rice heterologous cytoplasm fertility recovery QTL qRf5.1 and application of molecular marker

A fertility recovery and molecular marker technology, applied in DNA/RNA fragmentation, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of limiting high-yield and high-quality hybrid combination cultivation, single genetic background, etc., and achieve accelerated breeding. Work process, good stability, improve breeding efficiency and process effect

Pending Publication Date: 2022-06-28
江西省农业科学院水稻研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since the successful matching of three-line hybrid rice in my country, a number of ace backbone indica restorer lines such as IR24, IR26, IR30, Minghui 63, Yangdao 6 (9311) and Huazhan have emerged. However, the genetic background of these backbone restorer lines Single, most of its pedigree contains the genetic components of the farm variety Peta in Southeast Asia, which belongs to the medium indica ecotype of South Asia and Southeast Asia, which greatly limits the cultivation of high-yield and high-quality hybrid combinations

Method used

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  • Molecular marker of rice heterologous cytoplasm fertility recovery QTL qRf5.1 and application of molecular marker
  • Molecular marker of rice heterologous cytoplasm fertility recovery QTL qRf5.1 and application of molecular marker
  • Molecular marker of rice heterologous cytoplasm fertility recovery QTL qRf5.1 and application of molecular marker

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Experimental program
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Effect test

Embodiment 1

[0033] Example 1. Construction and development of Xieqingzao B / Dongxiang wild rice introgression line

[0034]Dongxiang wild rice has the recovery characteristics of various cytoplasmic male sterility. In the present invention, Dongxiang wild rice is used as the donor parent, and the dwarf-ruined maintainer line Xieqingzao B is further used as the recurrent parent to carry out hybridization, backcrossing and self-crossing to obtain one set of BC 1 F 10 Xieqingzao B / / Dongxiang wild rice / Xieqingzao B backcross recombined inbred population. The fertility restoration QTL qRf5.1 was detected on chromosome 5 of rice using this population, and the line carrying the target QTL qRf5.1 (line number 5339) was backcrossed with Xieqingzao B, and the prospect selection was carried out with the aid of molecular markers. and background selection, two sets of BCs were constructed by single-grain propagation method. 4 F 5 and BC 4 F 6 Daixie Qingzao B / Dongxiang wild rice introgression pop...

Embodiment 2

[0036] Embodiment 2, BC 4 F 5 and BC 4 F 6 Investigation on Seed Setting Rate of Daixie Qingzao B / Dongxiang Wild Rice Test Cross Population

[0037] In 2020 and 2021, test cross populations were planted in Nanchang Experimental Base of Rice Research Institute, Jiangxi Academy of Agricultural Sciences, with random block design, 3 replicates, 32 plants were planted in each block, and the transplanting size was 16.5cm×20cm. The water and fertilizer management was carried out as usual. At the maturity stage, 5 plants in the middle of each test cross material were selected for each repetition, and after they were naturally air-dried indoors, indoor artificial seed testing was carried out to investigate the related characters such as rice seed setting rate.

Embodiment 3

[0038] Example 3. Localization and verification of QTL qRf5.1 for fertility restoration in heterologous CMS lines

[0039] Using Mapmaker 3.0 software for 192 BCs 4 F 5 The genetic distance of the Xieqingzao B / Dongxiang wild rice population, the recombination rate was converted into the genetic distance using the Kosambi function, and the BC was constructed. 4 F 5 Linkage genetic map of populations. Using the software WindowsQTL Cartographer 2.5 on BC 4 F 5 QTL analysis was carried out on the genotype data of Xieqingzao B / Dongxiang wild rice population and the phenotype data of test cross population. Composite interval mapping (CIM) was used to determine whether there was a QTL with an LOD value of 2.5 as the detection threshold. Finally, a major QTL for fertility restoration was located between RF01 and RM188 on chromosome 5. The distance is 104.8cM~110.6cM, the physical distance is 21,378,343~22,671,210bp, and it is named qRf5.1.

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Abstract

The invention belongs to the technical field of rice breeding and molecular biology, and particularly relates to an SSR molecular marker of a major QTLs site for regulating and controlling rice heterologous cytoplasm sterility restoration and application of the SSR molecular marker (as shown in Figure 1). A forward primer sequence and a reverse primer sequence of the marker are respectively 5 '-GAGTGGAAATGGCACATTA-3' and 5 '-ATTTCACCTCCCTAT-3', PCR (Polymerase Chain Reaction) amplification is carried out on genome DNA (Deoxyribonucleic Acid) of a rice sample by utilizing the primers, and electrophoresis detection is carried out on an amplification product, so that a rice variety with the amplification band size of 202bp has a fertility recovery function. The method has important application value for screening and breeding the restoring line of the rice heterologous cytoplasmic sterile line.

Description

technical field [0001] The invention relates to the technical field of waterway breeding, in particular to a molecular marker of the heterologous cytoplasmic fertility restoration QTL qRf5.1 of rice and its application. Background technique [0002] Rice is one of the three most important food crops in the world. More than 50% of the world's population eat rice as the staple food. Therefore, the high and stable yield of rice is closely related to global food security and social stability. Heterosis utilization can effectively improve crop yield, quality and resistance. The successful matching of the three lines of rice has greatly promoted the utilization of rice heterosis and the large-scale promotion and application of hybrid rice in China, and its planting area accounts for 1 / 2 of the rice planting area (Yuan Longping, 2010). [0003] The discovery and utilization of rice cytoplasmic male sterility and restoration genes has greatly improved the research on hybrid rice in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125Y02A40/10
Inventor 胡标林吴婷李霞严松
Owner 江西省农业科学院水稻研究所