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Manufacturing method of animal tiny tissue paraffin section

A paraffin section and production method technology, applied in the biological field, can solve the problems of lack of standardized and reliable technical guidance, difficulty in the production of microscopic tissue pathological sections, etc., and achieve the effects of preventing floating, not easy to lose, and high definition.

Active Publication Date: 2022-07-01
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for making animal micro-tissue pathological slices in view of the difficulties in making micro-tissue pathological slices in the prior art and the lack of standardized and reliable technical guidance

Method used

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  • Manufacturing method of animal tiny tissue paraffin section
  • Manufacturing method of animal tiny tissue paraffin section
  • Manufacturing method of animal tiny tissue paraffin section

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1 Making mouse ovary paraffin sections

[0087] like Figure 1-2 , 5, 8-9, 13, 16-17, 20-21, kill the mice and dissect the mice, correctly identify the mouse ovarian tissue and quickly and completely collect the material, obtain 2 mouse ovarian tissues, and put them into 10 % neutral formaldehyde fixative for 48h. After the fixation is completed, remove the mouse ovarian tissue from the fixative and place it in a wide-mouthed bottle, tie the bottle mask with gauze and fasten it with a thread, and use a rubber tube to drain the tap water into the wide-mouthed bottle to allow the water to overflow from bottom to top. Achieve overflow flushing of tiny tissues.

[0088] After 2 hours of overflow rinsing, the mouse ovarian tissue was taken out, marked with eosin staining solution on the surface of the mouse ovary, and then wrapped with lens tissue to obtain tiny tissue wraps. The wrapping method is as follows: Wet the lens tissue with AF solution or water, and p...

Embodiment 2

[0099] Example 2 Preparation of mouse adrenal paraffin sections

[0100] like image 3 , 6 , 8, 10, 14, 16, 18, 22-23, mouse adrenal paraffin sections were prepared according to the slicing method of the present invention.

[0101] S1. Sampling: Correctly identify the adrenal glands of mice and take samples quickly and completely. One mouse has two intact adrenal tissues.

[0102] S2. Fixation: Put the mouse ovarian tissue into 10% neutral formaldehyde fixative solution for 48 hours; after fixation, remove the mouse ovarian tissue and place it in a wide-mouth bottle, tie the bottle mask with gauze and fasten it with a thread, and use a rubber The tube drains tap water into the jar, allowing the water to overflow from bottom to top to flush the mouse ovarian tissue.

[0103] S3. Marking and wrapping: After rinsing, the surface of mouse ovarian tissue was marked with eosin staining solution; after marking, it was wrapped with lens tissue to obtain micro-tissue wrapping.

...

Embodiment 3

[0111] Example 3 Making mouse ovary paraffin sections

[0112] The mice were sacrificed and dissected. The mouse ovarian tissue was correctly identified and obtained quickly and completely. Two mouse ovarian tissues were obtained, which were placed in a 10% neutral formaldehyde fixative solution for 48 hours. After the fixation is completed, remove the mouse ovarian tissue from the fixative and place it in a wide-mouthed bottle, tie the bottle mask with gauze and fasten it with a thread, and use a rubber tube to drain the tap water into the wide-mouthed bottle to allow the water to overflow from bottom to top. Achieve overflow flushing effect on tiny tissues. After overflow washing for 10 minutes, the mouse ovarian tissue was taken out, and the surface of the mouse ovary was marked with eosin staining solution, and then wrapped with lens tissue to obtain tiny tissue wraps.

[0113] Put the micro-tissues into the embedding box, cover the cover of the embedding box, and carry...

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Abstract

The invention relates to an animal tiny tissue paraffin section manufacturing method which comprises the following steps: correctly identifying animal tiny tissues, quickly and completely taking materials, putting the materials into neutral formaldehyde stationary liquid for fixation, and performing overflow flushing; marking with eosin dye liquor, and wrapping with lens wiping paper to obtain a tiny tissue package; putting into a container with holes, and carrying out ethanol gradient dehydration treatment; then putting into xylene for transparent treatment; molten wax liquid is injected into the embedding mold, the tiny tissue is put into the embedding mold, and the molten wax liquid continues to be injected; after the wax liquid is completely solidified, a wax block is trimmed and cut into a quadrangular frustum pyramid shape; freezing, and continuously slicing by using a passivated blade; spreading in pure water, adhering the section to a glass slide, baking the section, and dyeing to obtain the animal tiny tissue paraffin section. According to the method, eosin is used for marking in advance, wrapping protection is adopted, loss is not prone to occurring, the blade is treated through passivation, the slice quality is good, and the tiny tissue structure is clear and complete.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for making animal pathological slices, and more particularly, to a method for making animal micro-tissue paraffin slices. Background technique [0002] Generally speaking, the size of small mammalian tissues is only the size of rice grains, millet grains or sesame seeds, or even broken small tissues. As long as the tissues are visible to the naked eye and have a small long diameter, they are called "micro-tissues". Common tiny tissues in basic experiments such as: pituitary gland, ovary, adrenal gland, lymph nodes and other tissues of animals; common tiny tissues in clinical practice such as fiberoptic bronchoscopy (bronchoscopy), electronic fiberoptic gastroscope, pharyngoscope, colonoscopy, etc. Tissue, as well as breast, lung, liver, mediastinum, prostate, testis and other puncture and aspirated tissues. [0003] Because these tiny tissues are small in size, broken in qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/36G01N1/30
CPCG01N1/286G01N1/36G01N1/30G01N2001/2873
Inventor 王竹陈洁韩佳杞胡靖睿李稳王程仕贺涛黄芹杨清郑鸿王艳萍范羽王宇锁娇娇
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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