Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method and application of manganese nanoflower-aptamer probe

A technology of nanoflowers and aptamers, which is applied in the direction of analyzing materials by chemical reactions, material analysis by observing the influence of chemical indicators, color/spectral characteristic measurement, etc., which can solve the problems of expensive, time-consuming, cumbersome operation and other problems, to achieve the effect of improving detection accuracy and detection efficiency

Pending Publication Date: 2022-07-01
WUHAN POLYTECHNIC UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these methods have problems such as cumbersome operation, time-consuming and expensive, and cannot be widely used in OTA rapid on-site detection. Therefore, the development of fast, simple, sensitive and low-cost detection technology has attracted more and more attention from researchers.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of manganese nanoflower-aptamer probe
  • Preparation method and application of manganese nanoflower-aptamer probe
  • Preparation method and application of manganese nanoflower-aptamer probe

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] In view of this, in order to achieve the above object, the present invention proposes a preparation method of a manganese nanoflower-aptamer probe, comprising the following steps:

[0035] In step S10, oleic acid is added to the potassium permanganate solution, and a redox reaction is carried out to purify to obtain manganese nanoflowers.

[0036] The present invention uses potassium permanganate (KMnO 4 ) as a manganese precursor to prepare Manganese Nano Flowers (Mn-NFs). , which can rapidly catalyze the oxidation of chromogenic substrates and produce color changes. Taking the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) as an example, the Mn-NFs prepared by the present invention can catalyze the color reaction of TMB, turn blue, and the Mn-NFs after the color develops. The solution has an obvious ultraviolet absorption peak in the 600-700 nm band. Based on this, the absorbance of this band can be detected by ultraviolet absorption spectrophotometry, a...

Embodiment 1

[0053] Weigh 0.5g KMnO 4 Dissolve in 250 mL of distilled water, and rapidly stir the mixture for about 0.5 h to prepare potassium permanganate solution. Then 5.0 mL of OA was added, and the reaction was continued for 48 h. The crude brown-black product was collected by centrifugation, washed with ultrapure water and ethanol several times to remove residual reactants, and the honeycomb-shaped manganese nanoflower material (Mn-NFs) was obtained. see figure 1 , the electron microscope observation shows that Mn-NFs have uniform size and high specific surface area, indicating that they have high nanoenzyme activity and high specific surface area.

[0054] The purified Mn-NFs were redispersed in 250 mL of ultrapure water to obtain the manganese nanoflower dispersion.

[0055] The ochratoxin A aptamer (OTA-apt) was centrifuged, added with ultrapure water and vortexed for about 1 min to prepare a 1 μmol / L ochratoxin A aptamer solution for use.

[0056] Take 2 μL of the manganese n...

Embodiment 2

[0071] Weigh 0.5g KMnO 4 Dissolve in 250 mL of distilled water, and rapidly stir the mixture for about 0.5 h to prepare potassium permanganate solution. Then 4.0 mL of OA was added, and the reaction was continued for 40 h. The crude brown-black product was collected by centrifugation, washed with ultrapure water and ethanol several times to remove residual reactants, and the honeycomb-shaped manganese nanoflower material (Mn-NFs) was obtained. The purified Mn-NFs were redispersed in 250 mL of ultrapure water to obtain the manganese nanoflower dispersion.

[0072] The ochratoxin A aptamer (OTA-apt) was centrifuged, added with ultrapure water and vortexed for about 1 min to prepare a 1 μmol / L ochratoxin A aptamer solution for use.

[0073] Take 2 μL of the manganese nanoflower dispersion prepared in the above steps into a centrifuge tube, add 10 μL of ochratoxin A aptamer solution, and then add 50 μL of 1mol / L NaCl solution, 10 μL of PBS solution (pH7.5) and 28 μL of ultrapure ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method and application of a manganese nanoflower-aptamer probe, and the preparation method comprises the following steps: adding oleic acid into a potassium permanganate solution, carrying out an oxidation-reduction reaction, and purifying to obtain manganese nanoflowers; and dispersing the manganese nanoflower in water, adding an ochratoxin A aptamer solution, a sodium chloride solution, a phosphate buffer solution and water, uniformly mixing, and incubating to obtain the manganese nanoflower-aptamer probe. The probe provided by the invention has relatively strong catalytic activity on a chromogenic substrate 3, 3 ', 5, 5'-tetramethyl benzidine (TMB), and can quickly catalyze the chromogenic substrate to oxidize and generate color change, so that the chromogenic substrate can be used for detecting ochratoxin A through colorimetric or ultraviolet light absorption spectrophotometry, and the detection sensitivity of ochratoxin A is improved. By using the method, the detection accuracy and the detection efficiency of the ochratoxin A can be further improved.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a preparation method and application of a manganese nanoflower-aptamer probe. Background technique [0002] Ochratoxin A (OTA) is a mycotoxin that mainly exists in foods such as grains, nuts, soybeans, milk, fruit juices, wine, beer and edible oils, with a wide range of contamination. Due to the carcinogenic, teratogenic, mutagenic and immunosuppressive properties of OTA, excessive intake of OTA may lead to acute or chronic disease onset and even death. Therefore, it is necessary to strictly control and detect the content of ochratoxin A in agricultural products and food. [0003] At present, common OTA detection techniques include high-performance liquid chromatography, mass spectrometry, fluorescence spectroscopy, electrochemical methods, and Raman-enhanced spectroscopy, among others. Most of these methods have the problems of cumbersome operation, time-consumin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 蔡杰周娇娇吕旭琴
Owner WUHAN POLYTECHNIC UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com