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Complex microbial inoculant and remediation of petroleum-chlorophenol combined pollution environment by using complex microbial inoculant

A compound bacterial agent and compound pollution technology, applied in water pollutants, bacteria, biological water/sewage treatment, etc., can solve the problems of inability to degrade pollutants and weak degradation ability, and achieve obvious advantages in biodegradation and comprehensive functions. , easy to prepare

Pending Publication Date: 2022-07-15
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because these substances have strong toxicity or inhibition to functional microorganisms, the degradation ability of a single degrading bacteria is often weaker than that of a microbial combination, and the purpose of completely degrading pollutants cannot be achieved.

Method used

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  • Complex microbial inoculant and remediation of petroleum-chlorophenol combined pollution environment by using complex microbial inoculant
  • Complex microbial inoculant and remediation of petroleum-chlorophenol combined pollution environment by using complex microbial inoculant
  • Complex microbial inoculant and remediation of petroleum-chlorophenol combined pollution environment by using complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Strain screening

[0030] Materials and methods

[0031] Soil samples: Dongying Dongxin Oil Production Plant has long been contaminated by oil.

[0032] Sludge sample: activated sludge in the secondary sedimentation tank of Qingdao Sewage Treatment Plant.

[0033] Inorganic salt medium: (NH 4 ) 2 SO 4 1.0 g, K 2 HPO 4 1.0 g, KH 2 PO 4 1.0 g, anhydrous CaCl 2 0.01 g, MgSO 4 ·7H 2 O 0.2g, NaNO 3 5.0 g, FeCl 3 Trace amount, distilled water 1000mL, adjust pH to 7.2~7.5, sterilize at 121℃ for 20min before use.

[0034] Petroleum hydrocarbon degrading bacteria screening medium: add a certain amount of petroleum on the basis of inorganic salt medium.

[0035] Chlorophenol-degrading bacteria screening medium: add a certain amount of chlorophenol on the basis of inorganic salt medium.

[0036] Screening of Petroleum Hydrocarbon Degrading Strain

[0037] Weigh 10g of petroleum-contaminated soil and add it to a sterilized Erlenmeyer flask containing 90mL...

Embodiment 2

[0050] Example 2. Strain compounding

[0051] Materials and Methods

[0052] Strains: After screening, two petroleum hydrocarbon-degrading bacteria C4 and C5, and two chlorophenol-degrading bacteria L3 and L4 were obtained.

[0053] culture medium

[0054] Beef extract liquid medium, petroleum-chlorophenol inorganic salt medium

[0055] Assay method: prepare petroleum-chlorophenol inorganic salt medium (the concentration of chlorophenol in the medium is 50mg / L; the content of petroleum is 2g / L), sterilized for later use;

[0056] Two petroleum hydrocarbon-degrading bacteria Alcaligenes faecails Y5 C4 and Bacillus sp.7614 C5; two chlorophenol-degrading bacteria Bacillus marisflavi strain BCB4-1 L3, Bacillus sp. aquimaris T 172) L4, according to the inoculation amount of compound bacteria is 10%, the bacterial liquid is added in the inorganic salt liquid medium with chlorophenol concentration of 50mg / L, oil concentration of 2g / L, pH=7.5, and different V C4 :V C5 :V L3 :V ...

Embodiment 3

[0064] Example 3. Remediation of petroleum-chlorophenol compound polluted environment by compound bacterial agent

[0065] Experimental setup:

[0066] The petroleum inorganic salt medium was prepared, and after sterilization, an appropriate amount of chlorophenol compound was added for use.

[0067] The mixed fermentation broth of 10% (V:V) compound bacteria was added to the prepared medium containing petroleum-chlorophenol inorganic salt.

[0068] The inorganic salt medium without the inoculated strain was used as the blank control group.

[0069] All treatments were incubated at 35°C for 7 days at 160 r / min.

[0070] The organic phase of the cultured medium was extracted with n-hexane, dried over anhydrous sodium sulfate, the operation was repeated three times, and concentrated to 2 mL with a nitrogen blower, and relevant assays were carried out.

[0071] The infrared analysis of the remaining oil was determined by Fourier transform infrared spectroscopy (FTIR), and the ...

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Abstract

The invention belongs to the technical field of petroleum-chlorophenol combined pollution environment bioremediation in the environment, and particularly relates to a complex microbial inoculant, a preparation method of the complex microbial inoculant and application of the complex microbial inoculant in the field of petroleum-chlorophenol combined pollution environment. The invention provides two petroleum hydrocarbon degrading bacteria, which are respectively alcaligenes faecalis Y5 C4 and bacillus (Bacillus sp.7614) C5, and the two petroleum hydrocarbon degrading bacteria are screened from petroleum-polluted soil of Dongying Dongsheng oil extraction plants, and the two petroleum hydrocarbon degrading bacteria can be used for degrading the petroleum hydrocarbon in the petroleum-polluted soil of the Dongsheng Dongsheng oil extraction plants, and can be used for degrading the petroleum hydrocarbon in the petroleum-polluted soil of the Dongsheng Dongsheng oil extraction plants. The invention relates to two chlorophenol degrading bacteria which are respectively bacillus martensii BCB4-1 L3 and bacillus aquaticus T172 L4, and the two chlorophenol degrading bacteria are obtained by screening activated sludge in a secondary sedimentation tank of a sewage treatment plant in Qingdao city, and the two chlorophenol degrading bacteria are respectively bacillus martensii BCB4-1 L3 and bacillus aquaticus T172 L4 and are obtained by screening the activated sludge in the secondary sedimentation tank of the sewage treatment plant in Qingdao city. The synergistic effect of the complex microbial inoculants improves the degradation of petroleum and chlorophenol, and the complex microbial inoculants prepared from the four strains have good application prospects in the aspect of bioremediation of water and soil petroleum-chlorophenol combined pollution environments.

Description

technical field [0001] The invention belongs to the technical field of environmental bioremediation of petroleum-chlorophenol compound pollution in the environment, and relates to a kind of bioremediation of Alcaligenes faecails Y5 C4, Bacillus sp. 7614 C5, Bacillus marisflavi strain BCB4 -1) A compound bacterial agent composed of L3 and Bacillus aquimaris T 172 (Bacillus aquimaris T 172) L4, which specifically includes a preparation method of the compound bacterial agent and its application in the field of petroleum-chlorophenol complex polluted environment bioremediation. Background technique [0002] Oilfield well sites or petrochemical pollution sites with long operating years are not only seriously polluted by petroleum hydrocarbons, but also have a lot of organic chlorine compounds. Although the environment has a certain "self-purification" effect, it can degrade and transform some toxic organic pollutants, but in most cases, the natural decay process is very long and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/05C12R1/07C02F101/32C02F101/36C02F101/34
CPCC12N1/20C02F3/34C02F2101/32C02F2101/36C02F2101/345
Inventor 刘其友李婧孙烁张秀霞徐晨菲乔晨露郭龙飞唐明璐赵朝成
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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