Enzyme complex hydrogel prepared based on catalysis of glucose oxidase/amino acid metal complex and preparation method of enzyme complex hydrogel

A technology of glucose oxidase and metal complexes, applied in prosthesis, medical science and other directions, can solve problems such as biological toxicity, and achieve the effect of promoting cartilage regeneration, easy operation and good biological safety.

Pending Publication Date: 2022-07-22
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional photoinitiation and thermal initiation, due to the presence of initiators, will inevitably lead to certain biological toxicity, and require the assistance of external photothermal equipment

Method used

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  • Enzyme complex hydrogel prepared based on catalysis of glucose oxidase/amino acid metal complex and preparation method of enzyme complex hydrogel
  • Enzyme complex hydrogel prepared based on catalysis of glucose oxidase/amino acid metal complex and preparation method of enzyme complex hydrogel
  • Enzyme complex hydrogel prepared based on catalysis of glucose oxidase/amino acid metal complex and preparation method of enzyme complex hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Polysaccharide-grafted double bond: 2.08 g of chondroitin sulfate was added to 50 mL of deionized water, magnetically stirred for 2 hours to completely dissolve, and HCl (0.1 M) was slowly added dropwise to adjust the pH to 3.5. Then, 675 μL of glycidyl methacrylate (GMA) was added to the solution, and the solution was stirred for 24 hours in a water bath at 50°C. 100 mL of absolute ethanol was added to the reaction system, and a white crude product was precipitated. The crude product was dialyzed against deionized water for three days, and then freeze-dried (-65°C, 1 Pa) to obtain a white flocculent product.

[0035] 2) Prepare the precursor solution: mix 80 mg of polysaccharide grafted with double bonds, 30 mg of N,N-dimethylacrylamide, and then add 100 μL of ferrous glycinate (10 mM) and 50 μL of glucose oxidase (1000 U / mL) after mixing, deionized 800 μL of water was added to the sample bottle, shaken to dissolve it completely and mixed evenly to obtain the precu...

Embodiment 2

[0039] 1) Step is the same as step 1) in Example 1.

[0040] 2) Prepare the precursor solution: 80 mg of grafted double bond polysaccharide, 30 mg of N,N-dimethylacrylamide, and 100 μL of ferrous histidine (10 mM) and 50 μL of glucose oxidase (1000 U / mL) were added in sequence after mixing. 800 μL of deionized water was added to the sample bottle, shaken to dissolve it completely and mixed evenly to obtain the precursor liquid of the hydrogel.

[0041] 3) Preparation of hydrogel: Add 100 μL of glucose (100 mM) solution to the above precursor solution, shake immediately, and mix quickly to obtain a transparent self-supporting hydrogel. The gel formation time About 1s, the compressive modulus is 48.5kPa.

Embodiment 3

[0043] 1) Step is the same as step 1) in Example 1.

[0044] 2) Prepare the precursor solution: mix 80 mg of polysaccharide grafted with double bonds, 30 mg of N,N-dimethylacrylamide, and then add 100 μL of lysine molybdenum (10 mM) and 50 μL of glucose oxidase (1000 U / mL) in sequence to remove 800 μL of ionized water was added to the sample bottle, shaken to dissolve it completely and mixed evenly to obtain the precursor liquid of the hydrogel.

[0045] 3) Preparation of hydrogel: Add 100 μL of glucose (100 mM) solution to the above precursor solution, shake immediately, and mix quickly to obtain a transparent self-supporting hydrogel. The gel formation time About 3s, the compressive modulus is 65.3kPa.

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Abstract

The invention relates to tandem enzyme complex hydrogel, in particular to enzyme complex hydrogel rapidly prepared based on glucose oxidase / amino acid metal complex catalysis and a preparation method of the enzyme complex hydrogel, and the preparation method comprises the following steps: S1, fully dissolving a monomer polymerization system and tandem enzyme in deionized water to obtain a hydrogel precursor solution; and S2, adding a glucose solution into the hydrogel precursor solution obtained in the step S1 to obtain the enzyme complex hydrogel. Compared with the prior art, the enzyme complex hydrogel has the advantages that the natural enzyme and the mimic enzyme are combined for use, so that the second-level gelation of the hydrogel at a wound is realized, the enzyme complex hydrogel has good biological safety, can be injected in situ and is used for promoting cartilage regeneration, the second-level polymerization can be realized even under the physiological blood sugar concentration, and the cartilage regeneration can be promoted. The enzymatic hydrogel can be used for in-situ repair of irregular wounds, war wounds and other difficult-to-treat wounds, and has important significance and popularization value.

Description

technical field [0001] The invention relates to an enzyme complex hydrogel, in particular to an enzyme complex hydrogel rapidly prepared based on the catalysis of glucose oxidase / amino acid metal complex and a preparation method thereof. Background technique [0002] Because cartilage tissue does not contain or contains few blood vessels and lymphatics, it does not have the ability to regenerate. When cartilage tissue is damaged or missing, its self-repairing ability is very limited. As we all know, cartilage tissue damage is mostly irregular defect surface, and traditional filling materials are difficult to achieve a good fit with the defect site. [0003] Widely used hydrogels are potential filler materials due to their ease of shaping, three-dimensional hydrophilic networks, and desirable biocompatibility. Among them, injectable hydrogels have strong advantages in tissue engineering due to their unique sol-gel transition properties and tunable mechanical properties. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/50A61L27/22A61L27/16
CPCA61L27/52A61L27/50A61L27/227A61L27/16A61L2430/06A61L2400/06C08L89/00C08L51/02
Inventor 丁玲王启刚王霞张琦
Owner TONGJI UNIV
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