Compound microbial agent for promoting nitrogen utilization of crops as well as preparation method and application of compound microbial agent

A compound microbial agent and crop technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the problems of lack of diversity of functions, single strain, low transformation efficiency, etc.

Pending Publication Date: 2022-07-22
CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Based on this, it is necessary to provide a compound microbial agent that promotes nitrogen utilization in crops, a preparation method and an

Method used

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  • Compound microbial agent for promoting nitrogen utilization of crops as well as preparation method and application of compound microbial agent

Examples

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preparation example Construction

[0035] In a second aspect of the present application, a method for preparing a composite microbial inoculum is provided. The above-mentioned composite microbial inoculum can be prepared by fermenting each bacterium and each fungus separately, and then mixing the fermented liquid. In some of these embodiments, see figure 1 , the preparation method comprises the following steps:

[0036] S100: the seeds of each activated bacteria and the seeds of each activated fungi are respectively inoculated into corresponding fermenters at an inoculation concentration of 10% (V / V), and fermented; the bacteria include N. eutropha and Lactobacillus, The fungi include yeast, Aspergillus niger, and Trichoderma reesei.

[0037] S200: Mix the fermentation broths according to the weight percentages of the fermentation products to obtain the composite microbial inoculum.

[0038] In this case, the corresponding fermentation broths are obtained by culturing N. eutropha and Lactobacillus, yeast, As...

Embodiment 1

[0063] Nitrosospira, Lactobacillus, yeast, Aspergillus niger, and Trichoderma reesei were inoculated into the first medium, respectively, and cultured in a shaker (120 rpm, 35° C.) for activation. The first medium includes: glucose 0.3g / L, yeast extract powder 0.2g / L, KH 2 PO 4 0.01g / L, MgSO 4 0.01 g / L, the pH of the first culture medium is 5.5-6.0.

[0064] The seeds of the activated bacteria and the activated fungi were respectively inoculated into the corresponding fermenters for pure culture in the first medium, and the inoculation amount was 10% (V / V). Under the condition of 35°C, the aeration rate was adjusted to 1.0vvm (gas volume / liquid volume / min), the stirring rate was 50-150rpm, and the dissolved oxygen (DO) concentration was maintained at about 60-100% for fermentation.

[0065] After 24h of fermentation, the high-concentration sterile glucose solution (50%) is sent into the fermenter through the peristaltic pump for feeding, and the glucose concentration in the...

Embodiment 2

[0069] The difference from Example 1 is that the composite microbial inoculum comprises the following components by mass percentage: 30% of Nitrospirulina fermented product, 20% of Lactobacillus fermented product, 20% of yeast fermented product, Aspergillus niger Fermentation 20% and Trichoderma reesei Fermentation 20%. The rest are the same as in Example 1.

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Abstract

The invention discloses a compound microbial agent for promoting nitrogen utilization of crops, which is prepared from the following components in percentage by mass: 20 to 30 percent of nitrosospirillum leavening, 20 to 30 percent of lactobacillus leavening, 20 to 30 percent of saccharomycetes leavening, 10 to 20 percent of aspergillus niger leavening and 10 to 20 percent of trichoderma reesei leavening, each fermentation product comprises a corresponding bacterium and a metabolite produced by the corresponding bacterium. The compound microbial agent contains a plurality of microorganisms including bacteria, fungi and the like, and metabolites and enzymes thereof. Each strain has respective emphasis and can perform advantage complementation, so that the strain has the advantages of various functions and metabolites, no antagonism, difficulty in inhibition and the like; in addition, ammonium nitrogen can be promoted to be converted into nitrite nitrogen, insoluble phosphorus and calcium in soil can be activated to be in effective states, the nitrogen absorption and nitrogen metabolism level of hosts can be improved, and the growth of crops and the absorption of nutrient substances can be promoted.

Description

technical field [0001] The invention relates to the technical field of microbial inoculants, in particular to a composite microbial inoculum for promoting nitrogen utilization in crops, a preparation method and applications. Background technique [0002] In the process of crop cultivation, nitrogen is the most important element affecting growth and development, yield and quality. In the current production process, a large amount of nitrogen fertilizer is often applied to meet the demand for nitrogen in the process of crop growth. However, the utilization efficiency of nitrogen fertilizer by crops is only 10%-15%, and the remaining large amount of nitrogen is lost with groundwater or converted into nitrogen and returned to the atmosphere. [0003] Nitrogen reuse is particularly important for the development of new plant tissues. During the reproductive growth period, more than 50% of nitrogen comes from nitrogen reuse. Improving the nitrogen use efficiency of crops to ensur...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N1/16A01N63/20A01N63/32A01N63/34A01N63/38A01P21/00C12R1/225C12R1/645C12R1/685C12R1/885C12R1/01
CPCC12N1/20C12N1/14C12N1/16A01N63/20A01N63/32A01N63/34A01N63/38
Inventor 户正荣尹华群蔡海林方明曹明锋刘天波孔午圆刘勇军靳志丽周志成黎亮志
Owner CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION
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