Method for simultaneously accumulating, enriching and separating multiple arsenic forms in natural medicine on line based on micelle-solvent accumulation

A technology of natural medicine and separation method, which is applied in the preparation of test samples, etc., can solve the problem of reduced sensitivity of capillary short light path injection volume, achieve rapid detection, efficient detection and separation, and improve sensitivity

Pending Publication Date: 2022-07-22
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Capillary electrophoresis (CE) has strong separation performance, but the short path length and small injection volume of the capillary reduce its sensitivity

Method used

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  • Method for simultaneously accumulating, enriching and separating multiple arsenic forms in natural medicine on line based on micelle-solvent accumulation
  • Method for simultaneously accumulating, enriching and separating multiple arsenic forms in natural medicine on line based on micelle-solvent accumulation
  • Method for simultaneously accumulating, enriching and separating multiple arsenic forms in natural medicine on line based on micelle-solvent accumulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1. Investigate the influence of micelle species on detection effect

[0075] Wash and dry the kelp, crush and pass through a 100-mesh sieve, then accurately weigh 200 mg of each sample and place it in a 15 mL centrifuge tube, add 5 mL of 2% HNO 3 , sonicated for 1h (40°C), then shaken at room temperature for 2h, and finally centrifuged at 4000r / min for 15min. Extracts were neutralized with NaOH prior to analysis.

[0076] Fused silica capillaries were coated with 1% hemexonium bromide (HDMB) for 20 min to satisfy the condition of the same EOF orientation between anions and capillaries. The activated capillary column was rinsed with 100 mM borax buffer pH 9.2 for 5 minutes before injection. In the capillary column, 10mM micelles and 10mg / L target analyte were mixed and injected into the stack as a sample solution at -10kV for 60s, and then 100% MeOH5s was injected at -10kV at 50mbar, the scanned analytes were brought from the micelles to the MSS boundary, I...

Embodiment 2

[0079] Embodiment 2. investigate the influence of PAPS concentration on detection effect

[0080]On the basis of Example 1, PAPS was selected as the micelle, and the PAPS concentration was changed to 0, 5, 10, 20, 30, and 40 mM, and other conditions remained unchanged.

[0081] The concentration of micelles is a key parameter affecting the formation of micelles to the solvent packing boundary (MSSB). Therefore, the analysis of different PAPS concentrations in the sample solution, the results are as follows image 3 shown. When the surfactant concentration was varied from 0 to 20 mM, the peak areas of As(V), MMA and DMA gradually increased, while the peak area of ​​As(III) decreased sharply when the micelle concentration was varied from 5 to 10 mM. While the PAPS concentration was further increased from 20 mM to 40 mM, the peak heights for all four analytes decreased slightly. It was demonstrated that an increase in PAPS concentration negatively affects the formation of mice...

Embodiment 3

[0082] Embodiment 3. investigate the influence of methanol content on detection effect

[0083] On the basis of Example 1, PAPS was selected as the micelle, and the concentration of MeOH was changed to 40, 60, 80, 100% (v / v), and other conditions remained unchanged.

[0084] Methanol is usually an organic solvent in MSS, which affects the interaction between analyte and micelles, reversing the effective electrophoretic mobility for enrichment. This example superimposes two steps of MeOH injection alone, and optimizes the percentage of MeOH in the range of 40-100% (v / v) to obtain the best resolution and enrichment of analytes under other conditions.

[0085] The result is as Figure 4 shown. Except As(III), when the methanol concentration was 40% (v / v), the peak areas of the other three compounds were all smaller than those when 60% was used. However, when the MeOH content was 60%-100% (v / v), the peak intensities of the three compounds [As(V), MMA, DMA] decreased, and the mi...

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Abstract

The invention discloses a method for simultaneously accumulating, enriching and separating multiple arsenic forms in a natural medicine on line based on micelle-solvent accumulation. A zwitterionic surfactant PAPS is used for carrying out effective and sensitive two-step accumulation capillary zone electrophoresis CZE on As (V), As (III), MMA and DMA through electric injection and micellar-to-solvent accumulation MSS in co-electroosmotic flow co-EOF. The HDMB coating fuses the quartz capillary to satisfy the condition of the same EOF direction between the anion and the capillary. The background solution is a borax buffer solution. The micelle and the target analyte are injected and stacked through a field enhanced sample under negative pressure; methanol is injected, wherein the scanned analyte is brought by the micelles to the MSS boundary. According to the invention, arsenic in a natural drug is detected by using a micelle-to-solvent accumulation method through coating capillary electrophoresis, rapid detection of arsenic form is realized, and the sensitivity of on-line enrichment of a capillary electrophoresis sample is improved.

Description

technical field [0001] The invention belongs to the field of separation and enrichment of arsenic forms in natural medicines, and relates to a method for on-line enrichment of arsenic by capillary electrophoresis technology, in particular to a simultaneous online-on-line analysis of multiple arsenic forms in natural medicines based on micelle-solvent accumulation Stacking enrichment and separation methods. Background technique [0002] Arsenic (As) is a natural element in the earth's crust that contains more than 50 compounds of varying toxicity and is widely distributed in the atmosphere, water and land. As industrial pollution permeates every aspect of life, people are exposed to high concentrations of inorganic arsenic, the most toxic species. Chronic arsenic poisoning leading to cardiovascular disease, neurological disease, diabetes or skin, bladder and lung cancer can be caused by prolonged exposure to inorganic arsenic, mainly through drinking contaminated water, eati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/40G01N1/34
CPCG01N1/40G01N1/34G01N2001/4038
Inventor 曹君余亚玲
Owner HANGZHOU NORMAL UNIVERSITY
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