DPO RT-PCR primer group, detection method and kit for synchronously detecting five citrus viruses and application of DPO RT-PCR primer group, detection method and kit

Abstract

The invention discloses a DPO RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer group for synchronously detecting five citrus viruses, a detection method, a kit and application of the DPO RT-PCR primer group. The sequences of the primer group are shown as SEQ ID NO.1-SEQ ID NO.10. The invention further discloses a kit for synchronously detecting the five citrus viruses. Specific DPORT-PCR primers are designed for the citrus tristeza virus, the citrus shatter leaf virus, the citrus shatter peel virus, the citrus yellow vein clearing virus and the citrus leaf mottle virus, the detection primers can effectively remove primer dimer interference, the five viruses can be detected from a sample at the same time, the detection method is simple, convenient and efficient, and the detection time is short. The method can be used for detection only by using a common PCR instrument and an electrophoresis apparatus, has the characteristics of rapidness, economy, high accuracy and high sensitivity, can be used for large-scale screening of field citrus plants and epidemic prevention detection before seedling transportation, and provides technical support for healthy seedling detection and field screening of diseases.

Description

technical field [0001] The invention belongs to the technical field of plant virus detection, and in particular relates to a DPORT-PCR primer set, a detection method, a kit and an application for synchronous detection of five citrus viruses. Background technique [0002] With the continuous expansion of citrus planting area and the introduction and cultivation of new varieties, the transfer of seedlings between regions is frequent; due to the difficulty of supervision, various diseases and insect pests are carried and spread through seedlings and cause serious harm, among which viral diseases are particularly prominent. [0003] Citrus tristezavirus (CTV) causes citrus tree vigor decline, shoots and stems sink, fruit becomes smaller and other symptoms; The diseased trees of Citrus exocortis viroid (CEVd) are dwarfed, the new shoots are few and weak, the leaves are small and most of them are zinc-deficient, the diseased trees have many flowers, but the flowers and fruits are ...

AI Technical Summary

Problems solved by technology

[0002] With the continuous expansion of citrus planting area and the introduction of new varieties, the transfer of seedlings between regions is frequent; due to the difficulty of supervision, various diseases and insect pests are spread through seedlings and cause serious damage, among which viral diseases are particularly prominent

[0003] Citrus tristezavirus (CTV) causes symptoms such as citrus tree vigor decline, branch stem depression, and fruit shrinkage; Citrus tatter leafvirus (CTLV) causes plant yellowing and weakness, and in severe cases, the whole plant dies; Citrus exocortis viroid (CEVd) diseased trees are dwarfed, with few and weak new shoots, small leaves and most of them show symptoms of zinc deficiency, and the diseased trees have many flowers, but severe flower and fruit drop, and low yield; citrus yellow vein bright virus (Citrus yellow vein clearing virus, CYVCV) can infect most citrus species such as lemons, limes, and sweet oranges, and can cause varying degrees of vein brightening, chlorosis, and mosaic symptoms, and can cause severe leaf rolling in sugar oranges; After Citrus leaf blotchvirus (CLBV) infects citrus plants, it can cause citrange or pomelo as the scion on the rootstock to combine abnormally. The incompatibility between the scion of citrus and the rootstock will directly endanger the growth of citrus trees. This in turn causes significant economic losses to the citrus industry.

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