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Mycoplasma pneumoniae antibody detection kit and application thereof

A detection technology for mycoplasma pneumoniae and antibodies, applied in the biological field, can solve the problems of difficult storage and poor thermal stability, and achieve the effects of wide application, improved stability and high precision

Pending Publication Date: 2022-07-26
ZHUHAI LIVZON DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a Mycoplasma pneumoniae antibody detection kit and its application, to solve the problem that the Mycoplasma pneumoniae in vitro detection reagent in the prior art has poor thermal stability and difficult preservation

Method used

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  • Mycoplasma pneumoniae antibody detection kit and application thereof
  • Mycoplasma pneumoniae antibody detection kit and application thereof
  • Mycoplasma pneumoniae antibody detection kit and application thereof

Examples

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Effect test

preparation example Construction

[0067] 3. Preparation of streptavidin-coated magnetic microspheres:

[0068] (1) Preparation of acetic acid buffer with pH 3.6: Weigh 2.55 g of sodium acetate trihydrate and dissolve it in 4500 mL of purified water, then add 14 mL of acetic acid to mix, and make up to 5000 mL to obtain acetate buffer with pH of 3.6.

[0069] (2) Magnetic microsphere connection (magnetic microsphere connection CMC method):

[0070]In the magnetic microsphere, add the above-mentioned pH 3.6 acetate buffer suspension of the coating volume equivalent, wherein the magnetic microsphere concentration is 20mg / mL, and then add the 1-cyclohexyl-2-morpholinoethyl that the concentration is 10mg / mL Carbodiimide p-toluenesulfonate (CMC) was added with 12 μg streptavidin (SA) according to 1 mg of magnetic microspheres to form a reaction system. The above reaction system was put into a constant temperature shaking water bath box at 37°C for reaction for 24 hours.

[0071] (3) Cleaning of magnetic microspher...

Embodiment 1

[0096] In this example, a reaction system of streptavidin-coated magnetic microspheres and biotin-labeled P1M / P30A antigen was used.

[0097] Biotin-labeled P1M / P30A antigen: The working concentration of biotin is 100ng / mL, and the working concentration of P1M / P30A antigen is 500ng / mL. During the process of labeling the antigen, take 1.5mg of P1M / P30A antigen and adjust it to 1.5mL with dialysate, and stir at room temperature After dialysis for 3 hours, the molar ratio of biotin to P1M / P30A antigen was 10:1, and the biotin-labeled P1M / P30A antigen was labeled according to the aforementioned preparation process of biotin-labeled P1M / P30A antigen.

[0098] Streptavidin-coated magnetic microspheres: the diameter of the magnetic microspheres is 1 μm, the working concentration is 0.5 mg / mL, and the working concentration of streptavidin is 10 μg / mL. The process of making the balls is coated.

[0099] In this example, alkaline phosphatase (ALP)-labeled anti-human IgM antibody, NaOH ...

Embodiment 2

[0108] The AES concentration in the working solution of antigen and antibody in Example 1 was changed to 0.02 wt %, and other conditions were the same as those in Example 1.

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Abstract

The invention provides a mycoplasma pneumoniae antibody detection kit and application thereof, and relates to the technical field of biology. The kit comprises a mycoplasma pneumoniae fusion antigen working solution, wherein the mycoplasma pneumoniae fusion antigen working solution contains a mycoplasma pneumoniae fusion antigen and a diluent; the tracer-labeled antibody working solution contains an antibody IgG, IgM or IgA and a diluent; wherein the mycoplasma pneumoniae fusion antigen has an amino acid sequence as shown in SEQ ID No.3; the diluent is a phosphate buffer solution containing 0.02 to 1 weight percent of fatty alcohol polyoxyethylene ether sulfate salt. The kit disclosed by the invention adopts the mycoplasma pneumoniae fusion antigen, so that the specificity is stronger, the sensitivity is higher, and the thermal stability is better; besides, a sulfate salt type anionic surfactant fatty alcohol polyoxyethylene ether sulfate salt is added into the diluent, so that a stable and good microenvironment is provided for the fusion antigen, and the thermal stability of the kit antigen is further improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit for Mycoplasma pneumoniae antibodies and applications thereof. Background technique [0002] Mycoplasma pneumoniae (MP) is a prokaryotic cell without a cell wall, which can bind to the mannosamine pyruvate receptor of the host respiratory mucosa epithelial cells and produce toxins, resulting in pneumonia. The seroepidemiological study showed that 20.7% to 38.9% of patients with community-acquired pneumonia in China were caused by MP, ranking first among the pathogens of adult community-acquired pneumonia. In addition to the respiratory symptoms caused by MP, extrapulmonary manifestations such as meningitis, myocarditis, hemolytic anemia, and thrombocytopenic purpura can occur in 20% to 25% of cases. Timely and effective diagnosis can effectively control the aggravation of MP infection and reduce mortality. Therefore, the accurate diagnosis of MP has important clinic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/532G01N33/535G01N33/536G01N33/569G01N33/58G01N21/76
CPCG01N33/532G01N33/535G01N33/536G01N33/581G01N33/56933G01N21/76
Inventor 管亚楚杨晓光曾敏霞马玲
Owner ZHUHAI LIVZON DIAGNOSTICS
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