High-efficiency composting microbial inoculum based on gradient conversion of human excrement PPLC from organic matters as well as preparation method and application of high-efficiency composting microbial inoculum
An organic matter and composting technology, applied in the field of high-efficiency composting inoculants, can solve the problems of slow speed of human excrement, large nitrogen loss, complex composition, etc., and achieve the effects of saving labor, reducing nitrogen loss, and shortening heating time.
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Embodiment 1
[0032] Embodiment 1: the preparation method of high-efficiency composting bacterial agent
[0033] Step 1. Preparation of mixed microbial bacterial liquid
[0034] (1) Slope culture of strains
[0035] Bacillus highlandi, Bacillus halotolerant, Bacillus methylotrophic, Nitrogenobacter phaeotrophus, Bacillus cereus, Candida utilis, and Bacillus amyloliquefaciens in the seed preservation tube were respectively activated by slant, and then inoculated in Purification operation is carried out in a petri dish to obtain Bacillus highlandi, Bacillus halotolerant, Bacillus methylotrophic, Nitrogenococcus sphaeroides, Bacillus cereus, Candida utilis, and Bacillus amyloliquefaciens pure breeds, respectively, for use.
[0036] In step (1), the step of activating Bacillus highlandi on the slant is as follows: Bacillus highlandi is inoculated on the sterilized slant medium I through an inoculation loop, and cultured at 30° C. for 1 day.
[0037] In step (1), the step of activating the hal...
Embodiment 2
[0065] Embodiment 2: the preparation method of high-efficiency composting bacterial agent
[0066] Step 1. Preparation of mixed microbial bacterial liquid
[0067] (1) Slope culture of strains
[0068] Bacillus highlandi, Bacillus halotolerant, Bacillus methylotrophic, Nitrogenobacter phaeotrophus, Bacillus cereus, Candida utilis, and Bacillus amyloliquefaciens in the seed preservation tube were respectively activated by slant, and then inoculated in Purification operation is carried out in a petri dish to obtain Bacillus highlandi, Bacillus halotolerant, Bacillus methylotrophic, Nitrogenococcus sphaeroides, Bacillus cereus, Candida utilis, and Bacillus amyloliquefaciens pure breeds, respectively, for use.
[0069] In step (1), the step of activating Bacillus highlandi on the slant is as follows: Bacillus highlandi is inoculated on the sterilized slant medium I through an inoculation loop, and cultured at 30° C. for 2 days.
[0070] In step (1), the step of activating the ha...
Embodiment 3
[0098] Embodiment 3: the method that utilizes high-efficiency composting inoculum to carry out human excrement composting treatment
[0099]The feces were loaded into the fermentation device, mixed with straw powder to adjust the carbon-nitrogen ratio, and then the high-efficiency composting bacterial agent obtained in Example 2 of the present invention was inoculated into the composting system with 20% of the inoculum, and was periodically turned over to give oxygen.
[0100] In order to confirm the technical effect of the high-efficiency composting inoculum of the present invention in the composting of human feces, the effect obtained in Example 3 was compared with that of the control group (the control group was not inoculated with the inoculum, the same composting system was used, and the , oxygen supply) for comparison, and protein degradation effects after composting (see figure 1 ), polysaccharide degradation effect (see figure 2 ), cellulose degradation effect (see ...
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