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Preparation method of medical fiber with functions of reducing scar hyperplasia and easing pain

A scar hyperplasia and fiber technology, applied in the field of biomedical materials, to achieve the effects of increased stability, sustained and stable analgesic effect, and reduced dosage

Active Publication Date: 2022-08-05
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no literature report on the biomedical materials for trauma analgesia and scar repair at the same time in the early postoperative period

Method used

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  • Preparation method of medical fiber with functions of reducing scar hyperplasia and easing pain
  • Preparation method of medical fiber with functions of reducing scar hyperplasia and easing pain
  • Preparation method of medical fiber with functions of reducing scar hyperplasia and easing pain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] like figure 1 As shown, a medical fiber preparation method with functions of reducing scar hyperplasia and analgesia, comprising the following steps:

[0046] Step S1: Weigh 10 mg (1.60 nmol) of recombinant human epidermal growth factor (rhEGF for short), dissolve it in 2.0 ml pH 6.00 phosphate buffer solution, and add 15 mg (0.08 μmol) 1-(3-dimethylaminopropyl) -3-Ethylcarbodiimide hydrochloride (hereinafter referred to as EDC), and 9.2 mg (0.08 μmol) of N-hydroxysuccinimide (hereinafter referred to as NHS) in the above solution, ultrasonicated for 10 minutes, and then added 40 mg (0.8 nmol) of HA-NH2, placed in a shaker for 24 hours at room temperature, dialyzed with a 3Kd dialysis bag for 12 hours, and freeze-dried for later use.

[0047] Step S2: Dissolve 0.40g of gelatin and 0.10g of hyaluronic acid in a mixed solvent consisting of 2.25g of water and 2.25g of trifluoroethanol, sonicate until completely dissolved, and add 25 mg of the recombinant human epidermal gr...

Embodiment 2

[0053] The medical fiber prepared in Example 1 was investigated in vitro drug release behavior:

[0054] Accurately weigh 50 mg of the medical fiber prepared in Example 1, put it into a centrifuge tube, add a PBS buffer solution (0.1 mol / L) with pH=7.40 as a release medium, shake to balance, place the centrifuge tube at 37 °C, shake Three replicates were performed in parallel on a shaking shaker with a frequency of 100 rpm. Draw the dissolution medium at a preset time point, add the same volume of release medium, measure the drug release concentration of ropivacaine with UV-Vis spectrophotometer, and calculate the cumulative release rate, the results are as follows figure 2 . Depend on figure 2 It can be seen that ropivacaine exhibits stable and continuous drug release, and does not reach the peak drug release until 72 hours, showing good sustained-release performance.

Embodiment 3

[0056] Anesthesia and analgesia experiment was performed on the medical fiber prepared in Example 1:

[0057] Select adult male rats weighing 300-350 g, make an incision of about 1 cm on the right back after anesthesia, implant spinning fibers and suture the wound. A clamping test was performed on the rats implanted with the medical fiber prepared in Example 1 to determine the analgesic effect of the drug. Specifically, brief squeezes were applied at six different locations within a 1 cm radius around the entire incision, and the animal's response to each stimulus was recorded. The normal response to stimulation is a withdrawal response or reflex contraction of the muscle tissue at the point of stimulation, while a negative response is seen as no withdrawal or muscle contraction. The response score is the percentage of times the animal responded to the stimulus as a percentage of the total number of stimuli. They were performed once a day for one week after the operation. Th...

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Abstract

The invention relates to a preparation method of a medical fiber with functions of reducing scar hyperplasia and easing pain, which comprises the following steps: covalently binding a recombinant human epidermal growth factor to hyaluronic acid molecules to obtain a compound rhEGF-HA, preparing gel from the compound rhEGF-HA to serve as an electrostatic spinning external solution, and taking a solution in which polylactic acid wraps ropivacaine as an electrostatic spinning internal solution to prepare the medical fiber with the functions of reducing scar hyperplasia and easing pain. The core-shell type electrostatic spinning fiber with the effects of easing pain and repairing scars is prepared by means of coaxial electrostatic spinning. Wherein the electrostatic spinning fiber comprises the following components: coaxial electrostatic spinning external liquid, 5%-10% of gelatin, 0.5%-3.5% of hyaluronic acid, 0.01%-0.2% of rhEGF-HA and the balance of a mixed solvent of trifluoroethanol and water. According to the invention, the slow-release analgesic function of the local anesthetic is realized, meanwhile, the wound of a patient is subjected to early repair, the pain of the patient is reduced to the maximum extent, the postoperative pain is improved, the wound healing is accelerated, the hyperplasia of skin scars is relieved, and the slow-release analgesic local anesthetic can be used for manufacturing medical products such as medical sutures, surgical dressings, medical bandages, filling fibers and the like.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a method for preparing medical fibers with functions of reducing scar hyperplasia and analgesia. Background technique [0002] Postoperative pain is acute pain that occurs immediately after surgery and usually lasts from 3 days to a week. Inadequate postoperative pain management may increase the patient's oxygen consumption, increase the heart load, reduce postoperative pulmonary function, reduce gastrointestinal motility, delay the recovery of gastrointestinal function, interfere with hormone levels and lead to a series of psychological problems. Therefore, good analgesia plays a crucial role in the postoperative management of surgical patients. [0003] The catabolic, anabolic, and healing aberrations of surgical wounds can lead to the formation of skin scarring during wound healing. Scar not only affects the appearance of the skin of the patient's wound, but mor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/26A61L15/32A61L15/44A61L15/46A61L15/62D01D5/00D01D5/34D01F8/18D01F8/02D01F8/14D01F1/10
CPCA61L15/44A61L15/46A61L15/28A61L15/32A61L15/26A61L15/62D01F8/18D01F8/02D01F8/14D01F1/10D01D5/34D01D5/003D01D5/0092A61L2300/414A61L2300/402A61L2300/236A61L2300/412
Inventor 刘文韬许贯虹
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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