Supercharge Your Innovation With Domain-Expert AI Agents!

Application of BcSpd1 gene in prevention and treatment of plant gray mold and improvement of disease resistance

A technology of plant ash and cinerea, applied in the field of microbial genetic engineering, can solve problems such as unclear functions of melanin

Pending Publication Date: 2022-08-05
JILIN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Melanin is a polyphenolic polymer that widely exists in various fungi. It is found in many pathogenic fungi that melanin plays an important role in the interaction between pathogenic bacteria and host plants. Botrytis cinerea can also produce melanin. However, melanin in this The function in bacteria is not yet clear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of BcSpd1 gene in prevention and treatment of plant gray mold and improvement of disease resistance
  • Application of BcSpd1 gene in prevention and treatment of plant gray mold and improvement of disease resistance
  • Application of BcSpd1 gene in prevention and treatment of plant gray mold and improvement of disease resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1. Correlation analysis of BcSpd1 gene

[0052] The open reading frame of the BcSpd1 gene of Botrytis cinerea consists of 1504 nucleotides, including 3 exons, and the total length of the coding region is 1398 nucleotides. The encoded protein product consists of 465 amino acids. Prediction analysis of BcSpd1 gene in Botrytis cinerea genome website (http: / / fungi.ensembl.org / Botrytis_cinerea / Location / View?r=6:1774070-1777326; g=BCIN06G05230; db=core), BcSpd1 gene number BCIN06G05230, this gene has a conserved Zn 2 C 6 domain, which belongs to typical zinc cluster proteins (such as figure 1 shown).

Embodiment 2

[0053] Example 2. Knockout and genetic complementation of BcSpd1 gene

[0054] 1) Knockout vector construction

[0055] Primers were designed by DNAMAN and synthesized by Shanghai Sangon Biotechnology Co., Ltd., using primers BcSpd1-UP-F (5'-GGCGGCCTCGAGAGTCTAACCCTCAAGAGCCAG-3') and BcSpd1-UP-R (5'-ATGAGCTCGAATTGGAGTTCCAAGTTGAGTGAT-3'), The upstream 998 fragment of BcSpd1 gene was amplified using the genomic DNA of Botrytis cinerea strain B05.10; primers BcSpd1-DN-F (5'-GCTCTAGAGCCTCTTTTCAAGGCAGAACGGT-3') and BcSpd1-DN-R (5'-AACTGCAGTATCAACGCGAGCGCGAGCACT-3 '), using the genomic DNA of Botrytis cinerea strain B05.10 as a template to amplify the downstream 995bp fragment of the BcSpd1 gene. Reaction system (25 μL): Takara Primer STARMax DNA Polymerase (2×) 12.5 μL; upstream and downstream primers (10 μM) 1 μL each; template DNA 1 μL; ddH 2 O 9.5 μL. Amplification procedure: (1) 98°C for 10s; (2) 53°C for 15s; (3) 72°C for 10s to cycle 1-3 steps 35 times. The PCR products we...

Embodiment 3

[0065] Example 3. The role of BcSpd1 gene in the growth and development of Botrytis cinerea

[0066] B05.10, △BcSpd1, and △BcSpd1-C were stored at -80°C for activation on PDA medium, and after culturing upside down in a 25°C incubator for 2 days, a 6 mm uniform bacterial cake was punched out using a hole punch and inoculated. On a new PDA plate, the incubator was inverted at 25°C, and the colony diameter was measured every 12h and recorded by photographing. The results showed that the mycelial growth rate of △BcSpd1 was lower than that of the wild-type and apoplexy, and the difference became more and more obvious with the prolongation of time. At the 72nd hour, the average diameters of the wild-type and apoplexy colonies were 7.21 cm and 7.26 cm, respectively. The knockout body is only 5.94 cm, indicating that the growth rate of △BcSpd1 is slower than that of B05.10 (eg Figure 4 shown). It was shown that in wild-type strains, BcSpd1 positively regulates hyphal growth, and t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a BcSpd1 gene related to pathogenicity, growth and development of botrytis cinerea and application of the BcSpd1 gene in botrytis cinerea treatment. The deletion of the BcSpd1 gene causes the formation rate and pathogenicity of a botrytis cinerea infection structure to be remarkably reduced, meanwhile, sclerotium formation is blocked, oxalic acid secretion is reduced, melanin formation is increased, and the BcSpd1 gene is a gene necessary for botrytis cinerea to cause plant botrytis cinerea and plays an important role in growth and development and metabolite generation of the BcSpd1 gene. A compound capable of preventing the expression of the gene and the expression, modification and positioning of the protein of the gene is screened, so that the gray mold can be effectively controlled, and the development of a novel bactericide is facilitated. One important application of the BcSpd1 gene is that the expression of the gene and a protein product coded by the gene can be used as important candidate target sites and are used for designing and screening botrytis cinerea resisting medicaments.

Description

technical field [0001] The invention belongs to the technical field of microbial genetic engineering, and particularly relates to the application of the botrytis cinerea BcSpd1 gene in the control and disease resistance improvement of botrytis cinerea in plants. Background technique [0002] Botrytis cinerea, commonly known as Botrytis cinerea, is the causative agent of Botrytis cinerea, and has a phenotype of Botryotinia fuckeliana (de Bary) Whetzal, which is Botrytis ascomycota A member of the genus Dictyostelium; the asexual form is Botrytis cinerea Pers, a member of the genus Botrytis asexual fungi. Botrytis cinerea has a wide range of hosts and can infect more than 1,400 species of plants including Solanaceae, Cucurbitaceae, Rosaceae, Leguminosae, Vitaceae, etc., and damage the flowers, fruits, leaves and stems of the host plants. Botrytis cinerea can cause $10 billion to $100 billion in economic losses worldwide. Due to the wide range of hosts, serious harm in produc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C07K14/37C12N15/80C12N1/15C12N15/113A01G13/00C12R1/645
CPCC07K14/37C12N15/80C12N1/14C12N15/113A01G13/00C12N2310/141C12N2310/11
Inventor 刘守安陈虎臣张舒涵李雯玲
Owner JILIN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com